Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005179                                                              20     509
NP_000426                                                              17    3301
XP_005259981                                                           17    3301
NP_722575                                                              16    7355
NP_001548                                                              16    7355
NP_001161770                                                           16    7355
NP_004454                                                              16    7355
NP_066983                                                              16    7355
XP_005247180                                                           16    7355
XP_005246588                                                           16    7355
XP_005246587                                                           16    7355
NP_001170986                                                           16    7355
NP_542189                                                              16    7355
NP_859052                                                              15    9605
NP_055529                                                              15    9605
NP_006827                                                              15    9605
NP_060417                                                              15    9605
NP_057419                                                              15    9605
XP_005276193                                                           15    9605
XP_006724998                                                           15    9605
XP_006721055                                                           15    9605
XP_006721054                                                           15    9605
XP_006721053                                                           15    9605
XP_006721052                                                           15    9605
XP_006716107                                                           15    9605
XP_006716106                                                           15    9605
XP_005271430                                                           15    9605
NP_061116                                                              15    9605
>NP_005179
          Length = 906

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VPALLARRT 9
           VPA+LARRT
Sbjct: 781 VPAVLARRT 789
>NP_000426
          Length = 2321

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    VPALLAR 7
            VPALLAR
Sbjct: 1504 VPALLAR 1510
>XP_005259981
          Length = 2269

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    VPALLAR 7
            VPALLAR
Sbjct: 1452 VPALLAR 1458
>NP_722575
          Length = 346

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VPALLARRT 9
           VPA L+R+T
Sbjct: 311 VPAHLSRKT 319
>NP_001548
          Length = 360

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VPALLARRT 9
           +P LL RRT
Sbjct: 178 LPVLLFRRT 186
>NP_001161770
          Length = 360

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VPALLARRT 9
           +P LL RRT
Sbjct: 178 LPVLLFRRT 186
>NP_004454
          Length = 961

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PALLARR 8
          P LLARR
Sbjct: 40 PGLLARR 46
>NP_066983
          Length = 558

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   PALLARRT 9
           P LL RRT
Sbjct: 421 PVLLIRRT 428
>XP_005247180
          Length = 207

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VPALLARRT 9
           VPA L+R+T
Sbjct: 172 VPAHLSRKT 180
>XP_005246588
          Length = 360

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VPALLARRT 9
           +P LL RRT
Sbjct: 178 LPVLLFRRT 186
>XP_005246587
          Length = 360

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VPALLARRT 9
           +P LL RRT
Sbjct: 178 LPVLLFRRT 186
>NP_001170986
          Length = 525

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   PALLARRT 9
           P LL RRT
Sbjct: 388 PVLLIRRT 395
>NP_542189
          Length = 469

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   PALLARRT 9
           P LL RRT
Sbjct: 313 PVLLLRRT 320
>NP_859052
          Length = 698

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  PALLARRT 9
          PAL ARR+
Sbjct: 18 PALRARRS 25
>NP_055529
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    PALLAR 7
            PALLAR
Sbjct: 1123 PALLAR 1128
>NP_006827
          Length = 2671

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   PALLAR 7
           PALLAR
Sbjct: 688 PALLAR 693
>NP_060417
          Length = 888

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VPALLARRT 9
           +PA++ RR+
Sbjct: 520 IPAVMGRRS 528
>NP_057419
          Length = 600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  PALLAR 7
          PALLAR
Sbjct: 83 PALLAR 88
>XP_005276193
          Length = 144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PALLARR 8
          PAL ARR
Sbjct: 76 PALAARR 82
>XP_006724998
          Length = 600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  PALLAR 7
          PALLAR
Sbjct: 83 PALLAR 88
>XP_006721055
          Length = 673

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   PALLAR 7
           PALLAR
Sbjct: 334 PALLAR 339
>XP_006721054
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    PALLAR 7
            PALLAR
Sbjct: 1123 PALLAR 1128
>XP_006721053
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    PALLAR 7
            PALLAR
Sbjct: 1123 PALLAR 1128
>XP_006721052
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    PALLAR 7
            PALLAR
Sbjct: 1123 PALLAR 1128
>XP_006716107
          Length = 579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VPALLARR 8
           V ALLARR
Sbjct: 141 VRALLARR 148
>XP_006716106
          Length = 654

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VPALLARR 8
           V ALLARR
Sbjct: 141 VRALLARR 148
>XP_005271430
          Length = 144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PALLARR 8
          PAL ARR
Sbjct: 76 PALAARR 82
>NP_061116
          Length = 725

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VPALLARR 8
           V ALLARR
Sbjct: 133 VRALLARR 140
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.332    0.138    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 478,572
Number of extensions: 868
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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