Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004351                                                              20     390
NP_003791                                                              17    2528
NP_001273357                                                           17    2528
NP_001273355                                                           17    2528
XP_006715652                                                           17    2528
NP_001010909                                                           17    3301
XP_005275464                                                           17    3301
XP_005275463                                                           17    3301
XP_005275181                                                           17    3301
XP_005275180                                                           17    3301
NP_002194                                                              16    7355
NP_001186930                                                           15    9605
NP_077719                                                              15    9605
NP_060087                                                              15    9605
NP_004955                                                              15    9605
NP_000426                                                              15    9605
NP_001518                                                              15    9605
NP_001257293                                                           15    9605
NP_036322                                                              15    9605
NP_001257294                                                           15    9605
NP_000683                                                              15    9605
XP_005259981                                                           15    9605
XP_006713545                                                           15    9605
XP_006713544                                                           15    9605
XP_006711659                                                           15    9605
XP_005277493                                                           15    9605
XP_005277492                                                           15    9605
XP_005277490                                                           15    9605
XP_005277491                                                           15    9605
XP_005277489                                                           15    9605
XP_005270959                                                           15    9605
XP_005270958                                                           15    9605
XP_006710730                                                           15    9605
NP_003628                                                              15    9605
>NP_004351
          Length = 882

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VNTYNTAIA 9
           VNTYN AIA
Sbjct: 293 VNTYNAAIA 301
>NP_003791
          Length = 597

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NTYNTAIA 9
           N YNTA+A
Sbjct: 537 NAYNTAMA 544
>NP_001273357
          Length = 514

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NTYNTAIA 9
           N YNTA+A
Sbjct: 454 NAYNTAMA 461
>NP_001273355
          Length = 574

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NTYNTAIA 9
           N YNTA+A
Sbjct: 514 NAYNTAMA 521
>XP_006715652
          Length = 574

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NTYNTAIA 9
           N YNTA+A
Sbjct: 514 NAYNTAMA 521
>NP_001010909
          Length = 566

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NTYNTAI 8
           NT+NTA+
Sbjct: 510 NTFNTAV 516
>XP_005275464
          Length = 572

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NTYNTAI 8
           NT+NTA+
Sbjct: 516 NTFNTAV 522
>XP_005275463
          Length = 626

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NTYNTAI 8
           NT+NTA+
Sbjct: 570 NTFNTAV 576
>XP_005275181
          Length = 572

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NTYNTAI 8
           NT+NTA+
Sbjct: 516 NTFNTAV 522
>XP_005275180
          Length = 596

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NTYNTAI 8
           NT+NTA+
Sbjct: 540 NTFNTAV 546
>NP_002194
          Length = 1181

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    VNTYNTAI 8
            +NTYN  I
Sbjct: 1100 INTYNPEI 1107
>NP_001186930
          Length = 1235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   VNTYN 5
           VNTYN
Sbjct: 279 VNTYN 283
>NP_077719
          Length = 2471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   VNTYN 5
           VNTYN
Sbjct: 279 VNTYN 283
>NP_060087
          Length = 2555

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   VNTYN 5
           VNTYN
Sbjct: 276 VNTYN 280
>NP_004955
          Length = 482

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TYNTAIA 9
           TY TA+A
Sbjct: 313 TYETAVA 319
>NP_000426
          Length = 2321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   VNTYN 5
           VNTYN
Sbjct: 255 VNTYN 259
>NP_001518
          Length = 488

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TYNTAIA 9
           TY TA+A
Sbjct: 314 TYETAVA 320
>NP_001257293
          Length = 912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   VNTYN 5
           VNTYN
Sbjct: 870 VNTYN 874
>NP_036322
          Length = 902

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   VNTYN 5
           VNTYN
Sbjct: 860 VNTYN 864
>NP_001257294
          Length = 801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   VNTYN 5
           VNTYN
Sbjct: 759 VNTYN 763
>NP_000683
          Length = 517

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   VNTYN 5
           VNTYN
Sbjct: 470 VNTYN 474
>XP_005259981
          Length = 2269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   VNTYN 5
           VNTYN
Sbjct: 255 VNTYN 259
>XP_006713545
          Length = 900

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   VNTYN 5
           VNTYN
Sbjct: 858 VNTYN 862
>XP_006713544
          Length = 902

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   VNTYN 5
           VNTYN
Sbjct: 860 VNTYN 864
>XP_006711659
          Length = 1046

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   NTYNTAIA 9
           N YNT+++
Sbjct: 715 NAYNTSLS 722
>XP_005277493
          Length = 1105

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   NTYNTAIA 9
           N YNT+++
Sbjct: 774 NAYNTSLS 781
>XP_005277492
          Length = 1119

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   NTYNTAIA 9
           N YNT+++
Sbjct: 788 NAYNTSLS 795
>XP_005277490
          Length = 1143

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   NTYNTAIA 9
           N YNT+++
Sbjct: 850 NAYNTSLS 857
>XP_005277491
          Length = 1143

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   NTYNTAIA 9
           N YNT+++
Sbjct: 850 NAYNTSLS 857
>XP_005277489
          Length = 1181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   NTYNTAIA 9
           N YNT+++
Sbjct: 850 NAYNTSLS 857
>XP_005270959
          Length = 2432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   VNTYN 5
           VNTYN
Sbjct: 240 VNTYN 244
>XP_005270958
          Length = 2432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   VNTYN 5
           VNTYN
Sbjct: 240 VNTYN 244
>XP_006710730
          Length = 2432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   VNTYN 5
           VNTYN
Sbjct: 240 VNTYN 244
>NP_003628
          Length = 1167

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   NTYNTAIA 9
           N YNT+++
Sbjct: 836 NAYNTSLS 843
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.117    0.309 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 404,600
Number of extensions: 377
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package