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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              19     665
NP_005877                                                              17    3301
XP_006721186                                                           17    3301
XP_005255829                                                           17    3301
XP_006721185                                                           17    3301
XP_006721184                                                           17    3301
NP_065433                                                              17    4312
NP_055571                                                              16    7355
NP_001008938                                                           16    7355
NP_149038                                                              16    7355
XP_005274533                                                           16    7355
NP_001258779                                                           15    9605
NP_689594                                                              15    9605
NP_954863                                                              15    9605
XP_006718009                                                           15    9605
XP_006712319                                                           15    9605
NP_001186073                                                           15    9605
NP_570141                                                              15    9605
NP_001135440                                                           15    9605

>NP_004295
          Length = 1620

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VNTAFSQSN 9
            VN AFSQSN
Sbjct: 1476 VNMAFSQSN 1484



>NP_005877
          Length = 655

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VNTAFSQSN 9
           +  AFSQSN
Sbjct: 286 IGVAFSQSN 294



>XP_006721186
          Length = 658

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VNTAFSQSN 9
           +  AFSQSN
Sbjct: 286 IGVAFSQSN 294



>XP_005255829
          Length = 672

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VNTAFSQSN 9
           +  AFSQSN
Sbjct: 286 IGVAFSQSN 294



>XP_006721185
          Length = 675

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VNTAFSQSN 9
           +  AFSQSN
Sbjct: 286 IGVAFSQSN 294



>XP_006721184
          Length = 675

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VNTAFSQSN 9
           +  AFSQSN
Sbjct: 286 IGVAFSQSN 294



>NP_065433
          Length = 1091

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NTAFSQSN 9
           NT+FS SN
Sbjct: 716 NTSFSASN 723



>NP_055571
          Length = 1972

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VNTAFSQSN 9
           V+ AFSQ N
Sbjct: 720 VSMAFSQKN 728



>NP_001008938
          Length = 2032

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VNTAFSQSN 9
           V+ AFSQ N
Sbjct: 720 VSMAFSQKN 728



>NP_149038
          Length = 512

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VNTAFSQSN 9
          VNTA +Q N
Sbjct: 15 VNTATNQGN 23



>XP_005274533
          Length = 652

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   NTAFSQSN 9
           N++FS+SN
Sbjct: 586 NSSFSKSN 593



>NP_001258779
          Length = 193

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NTAFSQSN 9
           NT+F Q+N
Sbjct: 152 NTSFIQNN 159



>NP_689594
          Length = 3549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   TAFSQS 8
           TAFSQS
Sbjct: 467 TAFSQS 472



>NP_954863
          Length = 522

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NTAFSQSN 9
           NT+F Q+N
Sbjct: 481 NTSFIQNN 488



>XP_006718009
          Length = 249

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VNTAFSQ 7
           +NTAF+Q
Sbjct: 234 MNTAFNQ 240



>XP_006712319
          Length = 3308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   TAFSQS 8
           TAFSQS
Sbjct: 226 TAFSQS 231



>NP_001186073
          Length = 3327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   TAFSQS 8
           TAFSQS
Sbjct: 245 TAFSQS 250



>NP_570141
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NTAFSQSN 9
           NT+F Q+N
Sbjct: 404 NTSFIQNN 411



>NP_001135440
          Length = 411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NTAFSQSN 9
           NT+F Q+N
Sbjct: 370 NTSFIQNN 377



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.105    0.258 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 325,143
Number of extensions: 410
Number of successful extensions: 19
Number of sequences better than 10000.0: 19
Number of HSP's gapped: 19
Number of HSP's successfully gapped: 19
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)