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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              19     665
XP_005263735                                                           18    1482
NP_775790                                                              17    3301
XP_005251234                                                           17    3301
XP_005251233                                                           17    3301
NP_002431                                                              17    4312
NP_001010852                                                           17    4312
NP_085126                                                              16    5631
NP_001136116                                                           16    5631
XP_005246927                                                           16    5631
XP_005246925                                                           16    5631
XP_006712841                                                           16    5631
XP_005246924                                                           16    5631
XP_006712840                                                           16    5631
NP_001245210                                                           16    7355
NP_000242                                                              16    7355
NP_056197                                                              16    7355
XP_005267560                                                           16    7355
XP_005267559                                                           16    7355
XP_005264389                                                           16    7355
NP_002174                                                              15    9605
NP_001254642                                                           15    9605
NP_001252541                                                           15    9605
NP_057368                                                              15    9605
NP_996882                                                              15    9605
NP_653318                                                              15    9605
NP_002214                                                              15    9605
XP_005274838                                                           15    9605
XP_005274837                                                           15    9605
XP_005274489                                                           15    9605
XP_005274488                                                           15    9605
XP_006719127                                                           15    9605
XP_006713728                                                           15    9605

>NP_002430
          Length = 1137

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VMIITRPNM 9
           VMIIT PNM
Sbjct: 891 VMIITGPNM 899



>XP_005263735
          Length = 686

 Score = 18.1 bits (35), Expect =  1482
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VMIITRPNM 9
           +M+IT PN+
Sbjct: 677 IMVITNPNL 685



>NP_775790
          Length = 354

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  MIITRPNM 9
          MIITRP++
Sbjct: 59 MIITRPDI 66



>XP_005251234
          Length = 317

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  MIITRPNM 9
          MIITRP++
Sbjct: 59 MIITRPDI 66



>XP_005251233
          Length = 354

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  MIITRPNM 9
          MIITRP++
Sbjct: 59 MIITRPDI 66



>NP_002431
          Length = 936

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MIITRPNM 9
           +IIT PNM
Sbjct: 676 LIITGPNM 683



>NP_001010852
          Length = 327

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  MIITRPNM 9
          M+ITRP++
Sbjct: 37 MVITRPDI 44



>NP_085126
          Length = 1671

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   MIITRPN 8
           +++TRPN
Sbjct: 625 LVLTRPN 631



>NP_001136116
          Length = 1700

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   MIITRPN 8
           +++TRPN
Sbjct: 625 LVLTRPN 631



>XP_005246927
          Length = 1683

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   MIITRPN 8
           +++TRPN
Sbjct: 608 LVLTRPN 614



>XP_005246925
          Length = 1696

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   MIITRPN 8
           +++TRPN
Sbjct: 621 LVLTRPN 627



>XP_006712841
          Length = 1696

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   MIITRPN 8
           +++TRPN
Sbjct: 650 LVLTRPN 656



>XP_005246924
          Length = 1713

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   MIITRPN 8
           +++TRPN
Sbjct: 638 LVLTRPN 644



>XP_006712840
          Length = 1725

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   MIITRPN 8
           +++TRPN
Sbjct: 650 LVLTRPN 656



>NP_001245210
          Length = 868

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   IITRPNM 9
           IIT PNM
Sbjct: 600 IITGPNM 606



>NP_000242
          Length = 934

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   IITRPNM 9
           IIT PNM
Sbjct: 666 IITGPNM 672



>NP_056197
          Length = 2610

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    VMIITRPNM 9
            VMI+ RP++
Sbjct: 1704 VMILRRPSL 1712



>XP_005267560
          Length = 2563

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    VMIITRPNM 9
            VMI+ RP++
Sbjct: 1657 VMILRRPSL 1665



>XP_005267559
          Length = 2610

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    VMIITRPNM 9
            VMI+ RP++
Sbjct: 1704 VMILRRPSL 1712



>XP_005264389
          Length = 921

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   IITRPNM 9
           IIT PNM
Sbjct: 666 IITGPNM 672



>NP_002174
          Length = 378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VMIITRPNM 9
           + I+T PNM
Sbjct: 205 IEILTPPNM 213



>NP_001254642
          Length = 300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VMIITRPNM 9
           + I+T PNM
Sbjct: 127 IEILTPPNM 135



>NP_001252541
          Length = 2371

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  MIITRPN 8
          ++IT+PN
Sbjct: 80 LLITKPN 86



>NP_057368
          Length = 2376

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  MIITRPN 8
          ++IT+PN
Sbjct: 80 LLITKPN 86



>NP_996882
          Length = 1551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  MIITRPN 8
          ++IT+PN
Sbjct: 80 LLITKPN 86



>NP_653318
          Length = 311

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   IITRPNM 9
           I+TRP M
Sbjct: 137 ILTRPGM 143



>NP_002214
          Length = 2701

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   MIITRPN 8
           ++IT+PN
Sbjct: 496 VVITKPN 502



>XP_005274838
          Length = 377

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VMIITRPNM 9
           + I+T PNM
Sbjct: 204 IEILTPPNM 212



>XP_005274837
          Length = 378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VMIITRPNM 9
           + I+T PNM
Sbjct: 205 IEILTPPNM 213



>XP_005274489
          Length = 377

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VMIITRPNM 9
           + I+T PNM
Sbjct: 204 IEILTPPNM 212



>XP_005274488
          Length = 378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VMIITRPNM 9
           + I+T PNM
Sbjct: 205 IEILTPPNM 213



>XP_006719127
          Length = 1865

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   MIITRPN 8
           ++IT+PN
Sbjct: 496 VVITKPN 502



>XP_006713728
          Length = 264

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  IITRPNM 9
          I+TRP M
Sbjct: 90 ILTRPGM 96



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.338    0.143    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 564,153
Number of extensions: 408
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)