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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001157394                                                           17    2528
NP_001189451                                                           17    4312
NP_001007026                                                           16    5631
NP_004862                                                              16    5631
XP_006726836                                                           16    5631
XP_006725868                                                           16    5631
XP_006725749                                                           16    5631
XP_006723018                                                           16    5631
XP_005258128                                                           16    5631
XP_005258127                                                           16    5631
XP_006710104                                                           16    5631
XP_005275994                                                           16    5631
XP_005276302                                                           16    7355
XP_005276301                                                           16    7355
XP_005272367                                                           16    7355
XP_005272366                                                           16    7355
XP_005276014                                                           16    7355
XP_005276013                                                           16    7355
NP_612201                                                              15    9605
NP_114147                                                              15    9605
NP_060242                                                              15    9605
XP_005260150                                                           15    9605
XP_006721797                                                           15    9605
XP_006721796                                                           15    9605
XP_006718461                                                           15    9605
XP_006718460                                                           15    9605
NP_705834                                                              15    9605

>NP_001157394
          Length = 867

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MESHSSTR 9
           +E HSSTR
Sbjct: 848 LEDHSSTR 855



>NP_001189451
          Length = 767

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VMESHSSTR 9
           V+ESH  TR
Sbjct: 450 VLESHPRTR 458



>NP_001007026
          Length = 248

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SHSSTR 9
          SHSSTR
Sbjct: 38 SHSSTR 43



>NP_004862
          Length = 250

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SHSSTR 9
          SHSSTR
Sbjct: 38 SHSSTR 43



>XP_006726836
          Length = 372

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   ESHSSTR 9
           ESH STR
Sbjct: 343 ESHPSTR 349



>XP_006725868
          Length = 315

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  VMESHSS 7
          +M+SHSS
Sbjct: 23 IMQSHSS 29



>XP_006725749
          Length = 315

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  VMESHSS 7
          +M+SHSS
Sbjct: 23 IMQSHSS 29



>XP_006723018
          Length = 372

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   ESHSSTR 9
           ESH STR
Sbjct: 343 ESHPSTR 349



>XP_005258128
          Length = 298

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SHSSTR 9
          SHSSTR
Sbjct: 88 SHSSTR 93



>XP_005258127
          Length = 300

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SHSSTR 9
          SHSSTR
Sbjct: 88 SHSSTR 93



>XP_006710104
          Length = 372

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   ESHSSTR 9
           ESH STR
Sbjct: 343 ESHPSTR 349



>XP_005275994
          Length = 318

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SHSSTR 9
           SHSSTR
Sbjct: 165 SHSSTR 170



>XP_005276302
          Length = 344

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ESHSSTR 9
           ++HSSTR
Sbjct: 145 QTHSSTR 151



>XP_005276301
          Length = 368

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ESHSSTR 9
           ++HSSTR
Sbjct: 176 QTHSSTR 182



>XP_005272367
          Length = 344

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ESHSSTR 9
           ++HSSTR
Sbjct: 145 QTHSSTR 151



>XP_005272366
          Length = 368

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ESHSSTR 9
           ++HSSTR
Sbjct: 176 QTHSSTR 182



>XP_005276014
          Length = 344

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ESHSSTR 9
           ++HSSTR
Sbjct: 145 QTHSSTR 151



>XP_005276013
          Length = 368

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ESHSSTR 9
           ++HSSTR
Sbjct: 176 QTHSSTR 182



>NP_612201
          Length = 404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  VMESHSS 7
          ++ESHSS
Sbjct: 62 LLESHSS 68



>NP_114147
          Length = 703

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   MESHSSTR 9
           + SHSST+
Sbjct: 269 LRSHSSTQ 276



>NP_060242
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VMESHSSTR 9
           V+E+HS  R
Sbjct: 100 VLENHSKLR 108



>XP_005260150
          Length = 703

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   MESHSSTR 9
           + SHSST+
Sbjct: 269 LRSHSSTQ 276



>XP_006721797
          Length = 362

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  VMESHSS 7
          ++ESHSS
Sbjct: 20 LLESHSS 26



>XP_006721796
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  VMESHSS 7
          ++ESHSS
Sbjct: 54 LLESHSS 60



>XP_006718461
          Length = 1247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   MESHSSTR 9
           MES  STR
Sbjct: 798 MESEDSTR 805



>XP_006718460
          Length = 1392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   MESHSSTR 9
           MES  STR
Sbjct: 943 MESEDSTR 950



>NP_705834
          Length = 594

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MESHSST 8
           MES+SST
Sbjct: 178 MESNSST 184



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.301    0.102    0.248 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 584,087
Number of extensions: 798
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)