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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              20     509
NP_001263689                                                           20     509
NP_001119584                                                           20     509
NP_001119586                                                           20     509
NP_001263625                                                           20     509
NP_001119590                                                           20     509
NP_001263690                                                           20     509
NP_001119585                                                           20     509
NP_001263624                                                           20     509
NP_001119588                                                           20     509
NP_001119587                                                           20     509
NP_001119589                                                           20     509
NP_001263627                                                           20     509
NP_001263628                                                           20     509
NP_001263626                                                           20     509
XP_005259653                                                           17    4312
NP_064555                                                              17    4312
NP_064506                                                              16    5631
NP_001243621                                                           15    9605
NP_001243618                                                           15    9605
NP_001243614                                                           15    9605
NP_001243620                                                           15    9605
NP_001243617                                                           15    9605
NP_055606                                                              15    9605
NP_001243619                                                           15    9605
NP_001243622                                                           15    9605
NP_001243616                                                           15    9605
NP_038464                                                              15    9605
NP_995314                                                              15    9605
XP_005250100                                                           15    9605
XP_006712264                                                           15    9605
XP_006712263                                                           15    9605

>NP_000537
          Length = 393

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   MAIYKQSQ 9
           MAIYKQSQ
Sbjct: 160 MAIYKQSQ 167



>NP_001263689
          Length = 354

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   MAIYKQSQ 9
           MAIYKQSQ
Sbjct: 121 MAIYKQSQ 128



>NP_001119584
          Length = 393

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   MAIYKQSQ 9
           MAIYKQSQ
Sbjct: 160 MAIYKQSQ 167



>NP_001119586
          Length = 341

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   MAIYKQSQ 9
           MAIYKQSQ
Sbjct: 160 MAIYKQSQ 167



>NP_001263625
          Length = 302

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   MAIYKQSQ 9
           MAIYKQSQ
Sbjct: 121 MAIYKQSQ 128



>NP_001119590
          Length = 354

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   MAIYKQSQ 9
           MAIYKQSQ
Sbjct: 121 MAIYKQSQ 128



>NP_001263690
          Length = 354

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   MAIYKQSQ 9
           MAIYKQSQ
Sbjct: 121 MAIYKQSQ 128



>NP_001119585
          Length = 346

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   MAIYKQSQ 9
           MAIYKQSQ
Sbjct: 160 MAIYKQSQ 167



>NP_001263624
          Length = 307

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   MAIYKQSQ 9
           MAIYKQSQ
Sbjct: 121 MAIYKQSQ 128



>NP_001119588
          Length = 209

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  MAIYKQSQ 9
          MAIYKQSQ
Sbjct: 28 MAIYKQSQ 35



>NP_001119587
          Length = 261

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  MAIYKQSQ 9
          MAIYKQSQ
Sbjct: 28 MAIYKQSQ 35



>NP_001119589
          Length = 214

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  MAIYKQSQ 9
          MAIYKQSQ
Sbjct: 28 MAIYKQSQ 35



>NP_001263627
          Length = 182

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2 MAIYKQSQ 9
         MAIYKQSQ
Sbjct: 1 MAIYKQSQ 8



>NP_001263628
          Length = 187

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2 MAIYKQSQ 9
         MAIYKQSQ
Sbjct: 1 MAIYKQSQ 8



>NP_001263626
          Length = 234

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2 MAIYKQSQ 9
         MAIYKQSQ
Sbjct: 1 MAIYKQSQ 8



>XP_005259653
          Length = 562

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   VMAIYKQSQ 9
           +++IYKQ+Q
Sbjct: 152 LLSIYKQTQ 160



>NP_064555
          Length = 563

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   VMAIYKQSQ 9
           +++IYKQ+Q
Sbjct: 152 LLSIYKQTQ 160



>NP_064506
          Length = 1516

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  MAIYKQSQ 9
          +AIYKQ++
Sbjct: 74 LAIYKQTE 81



>NP_001243621
          Length = 520

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  VMAIYKQ 7
          VMA+YK+
Sbjct: 83 VMALYKK 89



>NP_001243618
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VMAIYKQ 7
           VMA+YK+
Sbjct: 182 VMALYKK 188



>NP_001243614
          Length = 610

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VMAIYKQ 7
           VMA+YK+
Sbjct: 214 VMALYKK 220



>NP_001243620
          Length = 571

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VMAIYKQ 7
           VMA+YK+
Sbjct: 134 VMALYKK 140



>NP_001243617
          Length = 580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VMAIYKQ 7
           VMA+YK+
Sbjct: 143 VMALYKK 149



>NP_055606
          Length = 651

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VMAIYKQ 7
           VMA+YK+
Sbjct: 214 VMALYKK 220



>NP_001243619
          Length = 580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VMAIYKQ 7
           VMA+YK+
Sbjct: 143 VMALYKK 149



>NP_001243622
          Length = 457

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  VMAIYKQ 7
          VMA+YK+
Sbjct: 20 VMALYKK 26



>NP_001243616
          Length = 603

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VMAIYKQ 7
           VMA+YK+
Sbjct: 166 VMALYKK 172



>NP_038464
          Length = 1128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    AIYKQS 8
            A+YKQS
Sbjct: 1118 AVYKQS 1123



>NP_995314
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    AIYKQS 8
            A+YKQS
Sbjct: 1124 AVYKQS 1129



>XP_005250100
          Length = 308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   IYKQSQ 9
           +YKQSQ
Sbjct: 285 LYKQSQ 290



>XP_006712264
          Length = 1126

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    AIYKQS 8
            A+YKQS
Sbjct: 1116 AVYKQS 1121



>XP_006712263
          Length = 1132

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    AIYKQS 8
            A+YKQS
Sbjct: 1122 AVYKQS 1127



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.121    0.298 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 338,910
Number of extensions: 204
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)