Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              19     869
NP_006022                                                              17    4312
NP_056270                                                              17    4312
XP_005261187                                                           17    4312
XP_005261186                                                           17    4312
XP_005261185                                                           17    4312
XP_005261184                                                           17    4312
XP_005261183                                                           17    4312
XP_005261182                                                           17    4312
XP_005261181                                                           17    4312
XP_006714963                                                           17    4312
XP_006714962                                                           17    4312
XP_005266010                                                           17    4312
NP_714921                                                              16    5631
NP_115543                                                              16    7355
XP_006721041                                                           16    7355
XP_006721040                                                           16    7355
XP_005255739                                                           16    7355
NP_115758                                                              15    9605
NP_001137282                                                           15    9605
NP_001268451                                                           15    9605
NP_001268449                                                           15    9605
NP_877954                                                              15    9605
NP_001268450                                                           15    9605
NP_115874                                                              15    9605
NP_542376                                                              15    9605
NP_004361                                                              15    9605
XP_005260916                                                           15    9605
XP_006715397                                                           15    9605
>NP_004251
          Length = 1208

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    VLVEFNELA 9
            VLVEF+ELA
Sbjct: 1026 VLVEFSELA 1034
>NP_006022
          Length = 3336

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    LVEFNELA 9
            L EFNELA
Sbjct: 1540 LDEFNELA 1547
>NP_056270
          Length = 557

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VEFNELA 9
           VEFN+LA
Sbjct: 300 VEFNQLA 306
>XP_005261187
          Length = 2706

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    LVEFNELA 9
            L EFNELA
Sbjct: 1551 LDEFNELA 1558
>XP_005261186
          Length = 3139

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    LVEFNELA 9
            L EFNELA
Sbjct: 1422 LDEFNELA 1429
>XP_005261185
          Length = 3229

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    LVEFNELA 9
            L EFNELA
Sbjct: 1433 LDEFNELA 1440
>XP_005261184
          Length = 3258

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    LVEFNELA 9
            L EFNELA
Sbjct: 1551 LDEFNELA 1558
>XP_005261183
          Length = 3268

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    LVEFNELA 9
            L EFNELA
Sbjct: 1551 LDEFNELA 1558
>XP_005261182
          Length = 3346

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    LVEFNELA 9
            L EFNELA
Sbjct: 1550 LDEFNELA 1557
>XP_005261181
          Length = 3347

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    LVEFNELA 9
            L EFNELA
Sbjct: 1551 LDEFNELA 1558
>XP_006714963
          Length = 552

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VEFNELA 9
           VEFN+LA
Sbjct: 295 VEFNQLA 301
>XP_006714962
          Length = 557

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VEFNELA 9
           VEFN+LA
Sbjct: 300 VEFNQLA 306
>XP_005266010
          Length = 557

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VEFNELA 9
           VEFN+LA
Sbjct: 300 VEFNQLA 306
>NP_714921
          Length = 680

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   VLVEFNELA 9
           +++EFNEL+
Sbjct: 106 LVMEFNELS 114
>NP_115543
          Length = 813

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   LVEFNE 7
           L+EFNE
Sbjct: 602 LIEFNE 607
>XP_006721041
          Length = 680

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   LVEFNE 7
           L+EFNE
Sbjct: 469 LIEFNE 474
>XP_006721040
          Length = 661

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   LVEFNE 7
           L+EFNE
Sbjct: 450 LIEFNE 455
>XP_005255739
          Length = 823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   LVEFNE 7
           L+EFNE
Sbjct: 612 LIEFNE 617
>NP_115758
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VLVEFNEL 8
           +LV FN+L
Sbjct: 442 ILVAFNDL 449
>NP_001137282
          Length = 329

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VLVEFNEL 8
          V VEFN+L
Sbjct: 60 VSVEFNKL 67
>NP_001268451
          Length = 793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VLVEFNEL 8
           +LV+F EL
Sbjct: 121 ILVDFKEL 128
>NP_001268449
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VLVEFNEL 8
           +LV+F EL
Sbjct: 121 ILVDFKEL 128
>NP_877954
          Length = 824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VLVEFNEL 8
           +LV+F EL
Sbjct: 121 ILVDFKEL 128
>NP_001268450
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VLVEFNEL 8
           +LV+F EL
Sbjct: 121 ILVDFKEL 128
>NP_115874
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VLVEFNEL 8
           +LV+F EL
Sbjct: 121 ILVDFKEL 128
>NP_542376
          Length = 1899

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    VEFNELA 9
            V++NELA
Sbjct: 1299 VDYNELA 1305
>NP_004361
          Length = 3063

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    VEFNELA 9
            V++NELA
Sbjct: 2463 VDYNELA 2469
>XP_005260916
          Length = 581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VLVEFNEL 8
           +LV+F EL
Sbjct: 121 ILVDFKEL 128
>XP_006715397
          Length = 2972

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    VEFNELA 9
            V++NELA
Sbjct: 2372 VDYNELA 2378
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.142    0.355 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 442,840
Number of extensions: 538
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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