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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000360                                                              17    3301
XP_005268094                                                           17    3301
XP_005270532                                                           17    4312
NP_777556                                                              17    4312
NP_689418                                                              16    7355
NP_003809                                                              16    7355
XP_006723723                                                           16    7355
NP_001026851                                                           15    9605
NP_004928                                                              15    9605
XP_006721527                                                           15    9605
XP_006721526                                                           15    9605
XP_005256542                                                           15    9605

>NP_000360
          Length = 764

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VLTLIIVAF 9
           VLTL IVAF
Sbjct: 586 VLTLNIVAF 594



>XP_005268094
          Length = 764

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VLTLIIVAF 9
           VLTL IVAF
Sbjct: 586 VLTLNIVAF 594



>XP_005270532
          Length = 130

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  VLTLIIVAF 9
          +LTL+++AF
Sbjct: 49 LLTLLLIAF 57



>NP_777556
          Length = 155

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  VLTLIIVAF 9
          +LTL+++AF
Sbjct: 49 LLTLLLIAF 57



>NP_689418
          Length = 839

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   VLTLIIVAF 9
           +LTL++VA+
Sbjct: 607 MLTLLLVAY 615



>NP_003809
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VLTLIIVAF 9
          +LTL ++AF
Sbjct: 74 ILTLAMIAF 82



>XP_006723723
          Length = 394

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VLTLIIVAF 9
          +LTL ++AF
Sbjct: 74 ILTLAMIAF 82



>NP_001026851
          Length = 400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VLTLIIV 7
           VLTLII+
Sbjct: 214 VLTLIII 220



>NP_004928
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VLTLIIV 7
           VLTLII+
Sbjct: 214 VLTLIII 220



>XP_006721527
          Length = 253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  VLTLIIV 7
          VLTLII+
Sbjct: 67 VLTLIII 73



>XP_006721526
          Length = 400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VLTLIIV 7
           VLTLII+
Sbjct: 214 VLTLIII 220



>XP_005256542
          Length = 400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VLTLIIV 7
           VLTLII+
Sbjct: 214 VLTLIII 220



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.356    0.166    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 247,777
Number of extensions: 227
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.0 bits)
S2: 28 (15.4 bits)