Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000312                                                              20     390
NP_066000                                                              17    3301
XP_006713781                                                           17    3301
NP_001278417                                                           15    9605
NP_001278418                                                           15    9605
NP_689567                                                              15    9605
NP_001013725                                                           15    9605
NP_001171134                                                           15    9605
NP_060621                                                              15    9605
NP_001171136                                                           15    9605
NP_001073920                                                           15    9605
XP_006723201                                                           15    9605
XP_005254450                                                           15    9605
XP_005254449                                                           15    9605
XP_006720168                                                           15    9605
XP_006717206                                                           15    9605
XP_005252108                                                           15    9605
XP_006712674                                                           15    9605
XP_006712673                                                           15    9605
XP_006712672                                                           15    9605
XP_005246722                                                           15    9605
NP_001171135                                                           15    9605
NP_005383                                                              15    9605
>NP_000312
          Length = 928

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VLNVLNLKA 9
           +LNVLNLKA
Sbjct: 517 ILNVLNLKA 525
>NP_066000
          Length = 771

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   VLNVLNL 7
           VLNVLNL
Sbjct: 216 VLNVLNL 222
>XP_006713781
          Length = 771

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   VLNVLNL 7
           VLNVLNL
Sbjct: 216 VLNVLNL 222
>NP_001278417
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  NVLNLKA 9
          N+LNL+A
Sbjct: 61 NILNLQA 67
>NP_001278418
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  NVLNLKA 9
          N+LNL+A
Sbjct: 54 NILNLQA 60
>NP_689567
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  NVLNLKA 9
          N+LNL+A
Sbjct: 54 NILNLQA 60
>NP_001013725
          Length = 1649

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    NVLNLK 8
            N+LNLK
Sbjct: 1130 NILNLK 1135
>NP_001171134
          Length = 2446

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLNVLNLKA 9
           VLNV+N  A
Sbjct: 628 VLNVINQNA 636
>NP_060621
          Length = 2472

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLNVLNLKA 9
           VLNV+N  A
Sbjct: 628 VLNVINQNA 636
>NP_001171136
          Length = 2446

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLNVLNLKA 9
           VLNV+N  A
Sbjct: 628 VLNVINQNA 636
>NP_001073920
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NVLNLKA 9
           N+LNL+A
Sbjct: 334 NILNLEA 340
>XP_006723201
          Length = 554

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  NVLNLKA 9
          N+LNL+A
Sbjct: 18 NILNLQA 24
>XP_005254450
          Length = 1505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    NVLNLK 8
            N+LNLK
Sbjct: 1130 NILNLK 1135
>XP_005254449
          Length = 1626

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    NVLNLK 8
            N+LNLK
Sbjct: 1130 NILNLK 1135
>XP_006720168
          Length = 218

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NVLNLKA 9
           N+LNL+A
Sbjct: 130 NILNLEA 136
>XP_006717206
          Length = 1062

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLNVLNLK 8
           VLNV NL+
Sbjct: 189 VLNVTNLE 196
>XP_005252108
          Length = 1097

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLNVLNLK 8
           VLNV NL+
Sbjct: 189 VLNVTNLE 196
>XP_006712674
          Length = 2436

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLNVLNLKA 9
           VLNV+N  A
Sbjct: 592 VLNVINQNA 600
>XP_006712673
          Length = 2441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLNVLNLKA 9
           VLNV+N  A
Sbjct: 597 VLNVINQNA 605
>XP_006712672
          Length = 2443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLNVLNLKA 9
           VLNV+N  A
Sbjct: 599 VLNVINQNA 607
>XP_005246722
          Length = 2472

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLNVLNLKA 9
           VLNV+N  A
Sbjct: 628 VLNVINQNA 636
>NP_001171135
          Length = 2446

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLNVLNLKA 9
           VLNV+N  A
Sbjct: 628 VLNVINQNA 636
>NP_005383
          Length = 1096

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLNVLNLK 8
           VLNV NL+
Sbjct: 189 VLNVTNLE 196
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.138    0.339 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 378,147
Number of extensions: 481
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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