Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004110                                                              20     509
XP_005270462                                                           17    4312
XP_006710373                                                           17    4312
XP_005270461                                                           17    4312
NP_443724                                                              17    4312
NP_536722                                                              16    5631
XP_005266835                                                           16    5631
NP_036419                                                              15    9605
NP_057687                                                              15    9605
NP_001275750                                                           15    9605
NP_001095271                                                           15    9605
NP_001095270                                                           15    9605
NP_085152                                                              15    9605
NP_003866                                                              15    9605
NP_001275752                                                           15    9605
NP_477518                                                              15    9605
XP_006714712                                                           15    9605
XP_005272064                                                           15    9605
XP_006714711                                                           15    9605
XP_006714710                                                           15    9605
XP_006713621                                                           15    9605
XP_006713620                                                           15    9605
XP_005270491                                                           15    9605
XP_005244774                                                           15    9605
XP_005244773                                                           15    9605
XP_005244772                                                           15    9605
XP_005244771                                                           15    9605
XP_006710392                                                           15    9605
>NP_004110
          Length = 993

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   LKMMTQLG 9
           LKMMTQLG
Sbjct: 662 LKMMTQLG 669
>XP_005270462
          Length = 460

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VLKMMTQLG 9
          VLK++ QLG
Sbjct: 77 VLKVLAQLG 85
>XP_006710373
          Length = 460

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VLKMMTQLG 9
          VLK++ QLG
Sbjct: 77 VLKVLAQLG 85
>XP_005270461
          Length = 460

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VLKMMTQLG 9
          VLK++ QLG
Sbjct: 77 VLKVLAQLG 85
>NP_443724
          Length = 460

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VLKMMTQLG 9
          VLK++ QLG
Sbjct: 77 VLKVLAQLG 85
>NP_536722
          Length = 405

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LKMMTQLG 9
           +KMMTQ G
Sbjct: 221 VKMMTQKG 228
>XP_005266835
          Length = 425

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LKMMTQLG 9
           +KMMTQ G
Sbjct: 241 VKMMTQKG 248
>NP_036419
          Length = 778

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLKMMTQLG 9
           +LK+M +LG
Sbjct: 463 LLKLMCELG 471
>NP_057687
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLKMMTQL 8
           VLK MT+L
Sbjct: 606 VLKWMTEL 613
>NP_001275750
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLKMMTQL 8
           VLKM TQ+
Sbjct: 114 VLKMETQM 121
>NP_001095271
          Length = 791

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLKMMTQL 8
           VLK MT+L
Sbjct: 510 VLKWMTEL 517
>NP_001095270
          Length = 887

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLKMMTQL 8
           VLK MT+L
Sbjct: 606 VLKWMTEL 613
>NP_085152
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLKMMTQLG 9
           +LK+M +LG
Sbjct: 467 LLKLMCELG 475
>NP_003866
          Length = 693

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VLKMMTQL 8
           VLKM+T++
Sbjct: 662 VLKMVTEI 669
>NP_001275752
          Length = 383

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VLKMMTQL 8
          VLKM TQ+
Sbjct: 19 VLKMETQM 26
>NP_477518
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLKMMTQL 8
           VLKM TQ+
Sbjct: 94  VLKMETQM 101
>XP_006714712
          Length = 819

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLKMMTQL 8
           VLK MT+L
Sbjct: 510 VLKWMTEL 517
>XP_005272064
          Length = 909

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLKMMTQL 8
           VLK MT+L
Sbjct: 628 VLKWMTEL 635
>XP_006714711
          Length = 937

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLKMMTQL 8
           VLK MT+L
Sbjct: 628 VLKWMTEL 635
>XP_006714710
          Length = 937

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLKMMTQL 8
           VLK MT+L
Sbjct: 628 VLKWMTEL 635
>XP_006713621
          Length = 622

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLKMMTQLG 9
           +LK+M +LG
Sbjct: 470 LLKLMCELG 478
>XP_006713620
          Length = 785

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLKMMTQLG 9
           +LK+M +LG
Sbjct: 470 LLKLMCELG 478
>XP_005270491
          Length = 383

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VLKMMTQL 8
          VLKM TQ+
Sbjct: 19 VLKMETQM 26
>XP_005244774
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLKMMTQLG 9
           +LK+M +LG
Sbjct: 126 LLKLMCELG 134
>XP_005244773
          Length = 792

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLKMMTQLG 9
           +LK+M +LG
Sbjct: 425 LLKLMCELG 433
>XP_005244772
          Length = 801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLKMMTQLG 9
           +LK+M +LG
Sbjct: 467 LLKLMCELG 475
>XP_005244771
          Length = 802

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLKMMTQLG 9
           +LK+M +LG
Sbjct: 435 LLKLMCELG 443
>XP_006710392
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLKMMTQLG 9
           +LK+M +LG
Sbjct: 467 LLKLMCELG 475
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.330    0.137    0.354 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 267,618
Number of extensions: 373
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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