Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001138389                                                           18    1935
NP_001138386                                                           18    1935
NP_000132                                                              18    1935
NP_075259                                                              18    1935
NP_001138385                                                           18    1935
XP_006717776                                                           18    1935
XP_006717773                                                           18    1935
XP_006717772                                                           18    1935
XP_006717771                                                           18    1935
NP_055426                                                              17    2528
XP_005248757                                                           17    2528
XP_006715468                                                           17    2528
XP_005248756                                                           17    2528
XP_006715467                                                           17    2528
NP_001191243                                                           17    4312
NP_000940                                                              17    4312
NP_001191245                                                           17    4312
NP_001191244                                                           17    4312
XP_006714547                                                           17    4312
NP_001273004                                                           16    7355
NP_004311                                                              16    7355
NP_775293                                                              16    7355
NP_004781                                                              16    7355
NP_695008                                                              16    7355
NP_695010                                                              16    7355
NP_695009                                                              16    7355
XP_005245660                                                           16    7355
NP_001073895                                                           16    7355
NP_003733                                                              15    9605
NP_001075155                                                           15    9605
NP_066999                                                              15    9605
NP_116242                                                              15    9605
XP_006712881                                                           15    9605
XP_006712880                                                           15    9605
NP_113645                                                              15    9605
>NP_001138389
          Length = 705

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  VLIVEYLQI 9
          VLI EYLQI
Sbjct: 86 VLIGEYLQI 94
>NP_001138386
          Length = 709

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  VLIVEYLQI 9
          VLI EYLQI
Sbjct: 86 VLIGEYLQI 94
>NP_000132
          Length = 821

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  VLIVEYLQI 9
          VLI EYLQI
Sbjct: 86 VLIGEYLQI 94
>NP_075259
          Length = 822

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  VLIVEYLQI 9
          VLI EYLQI
Sbjct: 86 VLIGEYLQI 94
>NP_001138385
          Length = 769

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  VLIVEYLQI 9
          VLI EYLQI
Sbjct: 86 VLIGEYLQI 94
>XP_006717776
          Length = 806

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VLIVEYLQI 9
           VLI EYLQI
Sbjct: 105 VLIGEYLQI 113
>XP_006717773
          Length = 807

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VLIVEYLQI 9
           VLI EYLQI
Sbjct: 105 VLIGEYLQI 113
>XP_006717772
          Length = 838

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VLIVEYLQI 9
           VLI EYLQI
Sbjct: 105 VLIGEYLQI 113
>XP_006717771
          Length = 839

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VLIVEYLQI 9
           VLI EYLQI
Sbjct: 105 VLIGEYLQI 113
>NP_055426
          Length = 5596

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    VLIVEYLQ 8
            +LIV+YLQ
Sbjct: 2371 ILIVQYLQ 2378

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VLIVEYLQ 8
           VLIV+YL+
Sbjct: 918 VLIVDYLK 925
>XP_005248757
          Length = 3591

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VLIVEYLQ 8
           +LIV+YLQ
Sbjct: 366 ILIVQYLQ 373
>XP_006715468
          Length = 3744

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VLIVEYLQ 8
           +LIV+YLQ
Sbjct: 519 ILIVQYLQ 526
>XP_005248756
          Length = 3905

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VLIVEYLQ 8
           +LIV+YLQ
Sbjct: 680 ILIVQYLQ 687
>XP_006715467
          Length = 5550

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    VLIVEYLQ 8
            +LIV+YLQ
Sbjct: 2371 ILIVQYLQ 2378

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VLIVEYLQ 8
           VLIV+YL+
Sbjct: 918 VLIVDYLK 925
>NP_001191243
          Length = 521

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LIVEYLQI 9
           L+VEYL++
Sbjct: 209 LLVEYLEV 216
>NP_000940
          Length = 622

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LIVEYLQI 9
           L+VEYL++
Sbjct: 310 LLVEYLEV 317
>NP_001191245
          Length = 376

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LIVEYLQI 9
           L+VEYL++
Sbjct: 310 LLVEYLEV 317
>NP_001191244
          Length = 349

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LIVEYLQI 9
           L+VEYL++
Sbjct: 310 LLVEYLEV 317
>XP_006714547
          Length = 622

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LIVEYLQI 9
           L+VEYL++
Sbjct: 310 LLVEYLEV 317
>NP_001273004
          Length = 869

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   IVEYLQ 8
           IVEYLQ
Sbjct: 562 IVEYLQ 567
>NP_004311
          Length = 994

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   IVEYLQ 8
           IVEYLQ
Sbjct: 687 IVEYLQ 692
>NP_775293
          Length = 1001

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   IVEYLQ 8
           IVEYLQ
Sbjct: 687 IVEYLQ 692
>NP_004781
          Length = 563

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLIVEYLQ 8
           VLI+ YLQ
Sbjct: 164 VLILNYLQ 171
>NP_695008
          Length = 550

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLIVEYLQ 8
           VLI+ YLQ
Sbjct: 164 VLILNYLQ 171
>NP_695010
          Length = 519

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLIVEYLQ 8
           VLI+ YLQ
Sbjct: 164 VLILNYLQ 171
>NP_695009
          Length = 506

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLIVEYLQ 8
           VLI+ YLQ
Sbjct: 164 VLILNYLQ 171
>XP_005245660
          Length = 1158

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLIVEYLQI 9
           VL+V YL I
Sbjct: 234 VLVVAYLMI 242
>NP_001073895
          Length = 1158

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLIVEYLQI 9
           VL+V YL I
Sbjct: 234 VLVVAYLMI 242
>NP_003733
          Length = 1321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLIVEYLQI 9
           VLI  Y+QI
Sbjct: 152 VLITGYIQI 160
>NP_001075155
          Length = 154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  VLIVEYLQ 8
          VLI+++LQ
Sbjct: 9  VLIIQHLQ 16
>NP_066999
          Length = 355

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  VLIVEYLQ 8
          VLI+++LQ
Sbjct: 9  VLIIQHLQ 16
>NP_116242
          Length = 711

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LIVEYLQ 8
           L+V+YLQ
Sbjct: 628 LVVKYLQ 634
>XP_006712881
          Length = 1335

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLIVEYLQI 9
           VLI  Y+QI
Sbjct: 166 VLITGYIQI 174
>XP_006712880
          Length = 1335

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLIVEYLQI 9
           VLI  Y+QI
Sbjct: 166 VLITGYIQI 174
>NP_113645
          Length = 250

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLIVEYLQI 9
           VL+ EYL I
Sbjct: 93  VLVGEYLSI 101
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.338    0.160    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 461,225
Number of extensions: 644
Number of successful extensions: 37
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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