Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              19     665
XP_006716142                                                           19     665
XP_005250088                                                           19     665
XP_006716141                                                           19     665
XP_005250085                                                           19     665
XP_006716140                                                           19     665
XP_005250084                                                           19     665
XP_005250083                                                           19     665
XP_005250082                                                           19     665
NP_004522                                                              17    3301
NP_057460                                                              17    4312
NP_001244928                                                           17    4312
XP_006721607                                                           17    4312
XP_005256736                                                           17    4312
NP_061330                                                              16    7355
NP_620482                                                              15    9605
NP_001077084                                                           15    9605
NP_001276100                                                           15    9605
NP_001276099                                                           15    9605
XP_006723434                                                           15    9605
XP_006718995                                                           15    9605
XP_005274425                                                           15    9605
XP_006718994                                                           15    9605
XP_006718993                                                           15    9605
XP_005274424                                                           15    9605
XP_005274423                                                           15    9605
XP_006712362                                                           15    9605
NP_203753                                                              15    9605
NP_001005289                                                           15    9605
NP_001276102                                                           15    9605
>NP_733751
          Length = 4911

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VLIAVSSQH 9
           +LIAVSSQH
Sbjct: 601 LLIAVSSQH 609
>XP_006716142
          Length = 4927

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VLIAVSSQH 9
           +LIAVSSQH
Sbjct: 602 LLIAVSSQH 610
>XP_005250088
          Length = 4928

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VLIAVSSQH 9
           +LIAVSSQH
Sbjct: 602 LLIAVSSQH 610
>XP_006716141
          Length = 4959

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VLIAVSSQH 9
           +LIAVSSQH
Sbjct: 602 LLIAVSSQH 610
>XP_005250085
          Length = 4979

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VLIAVSSQH 9
           +LIAVSSQH
Sbjct: 602 LLIAVSSQH 610
>XP_006716140
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VLIAVSSQH 9
           +LIAVSSQH
Sbjct: 602 LLIAVSSQH 610
>XP_005250084
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VLIAVSSQH 9
           +LIAVSSQH
Sbjct: 602 LLIAVSSQH 610
>XP_005250083
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VLIAVSSQH 9
           +LIAVSSQH
Sbjct: 601 LLIAVSSQH 609
>XP_005250082
          Length = 4983

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VLIAVSSQH 9
           +LIAVSSQH
Sbjct: 602 LLIAVSSQH 610
>NP_004522
          Length = 188

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VLIAVSSQH 9
           ++IAVSS H
Sbjct: 135 IIIAVSSAH 143
>NP_057460
          Length = 1170

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IAVSSQH 9
           +A+SSQH
Sbjct: 810 VAISSQH 816
>NP_001244928
          Length = 1211

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IAVSSQH 9
           +A+SSQH
Sbjct: 851 VAISSQH 857
>XP_006721607
          Length = 1130

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IAVSSQH 9
           +A+SSQH
Sbjct: 810 VAISSQH 816
>XP_005256736
          Length = 1169

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IAVSSQH 9
           +A+SSQH
Sbjct: 809 VAISSQH 815
>NP_061330
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VLIAVSSQH 9
          VLI +SS H
Sbjct: 74 VLIEISSTH 82
>NP_620482
          Length = 1341

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AVSSQH 9
           A+SSQH
Sbjct: 619 AISSQH 624
>NP_001077084
          Length = 1314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AVSSQH 9
           A+SSQH
Sbjct: 619 AISSQH 624
>NP_001276100
          Length = 720

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  LIAVSSQH 9
          L+AV S+H
Sbjct: 8  LVAVGSKH 15
>NP_001276099
          Length = 720

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  LIAVSSQH 9
          L+AV S+H
Sbjct: 8  LVAVGSKH 15
>XP_006723434
          Length = 771

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  LIAVSSQH 9
          L+AV S+H
Sbjct: 59 LVAVGSKH 66
>XP_006718995
          Length = 2382

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   IAVSSQH 9
           IAVSS H
Sbjct: 700 IAVSSDH 706
>XP_005274425
          Length = 2566

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   IAVSSQH 9
           IAVSS H
Sbjct: 933 IAVSSDH 939
>XP_006718994
          Length = 2567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   IAVSSQH 9
           IAVSS H
Sbjct: 933 IAVSSDH 939
>XP_006718993
          Length = 2602

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   IAVSSQH 9
           IAVSS H
Sbjct: 920 IAVSSDH 926
>XP_005274424
          Length = 2614

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   IAVSSQH 9
           IAVSS H
Sbjct: 933 IAVSSDH 939
>XP_005274423
          Length = 2615

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   IAVSSQH 9
           IAVSS H
Sbjct: 933 IAVSSDH 939
>XP_006712362
          Length = 1350

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AVSSQH 9
           A+SSQH
Sbjct: 628 AISSQH 633
>NP_203753
          Length = 2601

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   IAVSSQH 9
           IAVSS H
Sbjct: 920 IAVSSDH 926
>NP_001005289
          Length = 320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLIAVSSQH 9
           +LIAVS  H
Sbjct: 220 ILIAVSYAH 228
>NP_001276102
          Length = 771

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  LIAVSSQH 9
          L+AV S+H
Sbjct: 59 LVAVGSKH 66
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.124    0.306 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 313,145
Number of extensions: 448
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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