Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              18    1135
XP_005262424                                                           18    1135
XP_006720235                                                           17    2528
NP_997290                                                              17    2528
NP_004560                                                              17    2528
NP_004332                                                              17    3301
XP_005264615                                                           17    3301
XP_006712164                                                           17    3301
XP_005264614                                                           17    3301
XP_005264613                                                           17    3301
XP_005264612                                                           17    3301
NP_001091896                                                           16    5631
NP_001127854                                                           16    5631
NP_055684                                                              16    5631
XP_005257661                                                           16    5631
NP_078966                                                              16    7355
XP_006723004                                                           16    7355
NP_689846                                                              15    9605
NP_001018123                                                           15    9605
NP_065924                                                              15    9605
XP_005265408                                                           15    9605

>NP_722576
          Length = 3080

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VLGIRMTSS 9
            VLG RMTSS
Sbjct: 1865 VLGTRMTSS 1873



>XP_005262424
          Length = 2875

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VLGIRMTSS 9
            VLG RMTSS
Sbjct: 1660 VLGTRMTSS 1668



>XP_006720235
          Length = 184

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  LGIRMTSS 9
          LGI+MTSS
Sbjct: 92 LGIQMTSS 99



>NP_997290
          Length = 438

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   GIRMTSS 9
           GIRMTSS
Sbjct: 187 GIRMTSS 193



>NP_004560
          Length = 188

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   LGIRMTSS 9
           LGI+MTSS
Sbjct: 96  LGIQMTSS 103



>NP_004332
          Length = 2225

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VLGIRMTSS 9
            VLG+ MTS+
Sbjct: 1277 VLGVEMTST 1285



>XP_005264615
          Length = 2162

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VLGIRMTSS 9
            VLG+ MTS+
Sbjct: 1214 VLGVEMTST 1222



>XP_006712164
          Length = 2179

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VLGIRMTSS 9
            VLG+ MTS+
Sbjct: 1214 VLGVEMTST 1222



>XP_005264614
          Length = 2191

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VLGIRMTSS 9
            VLG+ MTS+
Sbjct: 1277 VLGVEMTST 1285



>XP_005264613
          Length = 2208

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VLGIRMTSS 9
            VLG+ MTS+
Sbjct: 1277 VLGVEMTST 1285



>XP_005264612
          Length = 2242

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VLGIRMTSS 9
            VLG+ MTS+
Sbjct: 1277 VLGVEMTST 1285



>NP_001091896
          Length = 531

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VLGIRMT 7
           VLGIR+T
Sbjct: 525 VLGIRLT 531



>NP_001127854
          Length = 1114

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GIRMTSS 9
           GIR+TSS
Sbjct: 810 GIRLTSS 816



>NP_055684
          Length = 963

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GIRMTSS 9
           GIR+TSS
Sbjct: 824 GIRLTSS 830



>XP_005257661
          Length = 456

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VLGIRMT 7
           VLGIR+T
Sbjct: 450 VLGIRLT 456



>NP_078966
          Length = 14507

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LGIRMTSS 9
            LG+R TSS
Sbjct: 8121 LGLRKTSS 8128



>XP_006723004
          Length = 6443

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LGIRMTSS 9
          LG+R TSS
Sbjct: 35 LGLRKTSS 42



>NP_689846
          Length = 859

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLGIRMTSS 9
           V+G++ TSS
Sbjct: 488 VVGVKTTSS 496



>NP_001018123
          Length = 859

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLGIRMTSS 9
           V+G++ TSS
Sbjct: 488 VVGVKTTSS 496



>NP_065924
          Length = 716

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VLGIRMTS 8
          VLG+ MTS
Sbjct: 14 VLGLLMTS 21



>XP_005265408
          Length = 716

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VLGIRMTS 8
          VLG+ MTS
Sbjct: 14 VLGLLMTS 21



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.132    0.326 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 350,244
Number of extensions: 416
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)