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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           18    1482
NP_000242                                                              18    1482
XP_005264389                                                           18    1482
NP_037510                                                              17    3301
NP_001077083                                                           16    5631
XP_006721083                                                           16    5631
NP_055568                                                              15    9605
XP_005271905                                                           15    9605
XP_005271904                                                           15    9605
XP_005271903                                                           15    9605

>NP_001245210
          Length = 868

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   VLAQLDAV 8
           VLAQLDAV
Sbjct: 532 VLAQLDAV 539



>NP_000242
          Length = 934

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   VLAQLDAV 8
           VLAQLDAV
Sbjct: 598 VLAQLDAV 605



>XP_005264389
          Length = 921

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   VLAQLDAV 8
           VLAQLDAV
Sbjct: 598 VLAQLDAV 605



>NP_037510
          Length = 123

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1  VLAQLDAV 8
          VLAQLDA+
Sbjct: 21 VLAQLDAL 28



>NP_001077083
          Length = 523

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AQLDAVS 9
           AQLDA+S
Sbjct: 433 AQLDAIS 439



>XP_006721083
          Length = 534

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AQLDAVS 9
           AQLDA+S
Sbjct: 433 AQLDAIS 439



>NP_055568
          Length = 1282

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VLAQLDAVS 9
            +LA LDA+S
Sbjct: 1207 ILALLDALS 1215



>XP_005271905
          Length = 1219

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VLAQLDAVS 9
            +LA LDA+S
Sbjct: 1144 ILALLDALS 1152



>XP_005271904
          Length = 1219

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VLAQLDAVS 9
            +LA LDA+S
Sbjct: 1144 ILALLDALS 1152



>XP_005271903
          Length = 1282

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VLAQLDAVS 9
            +LA LDA+S
Sbjct: 1207 ILALLDALS 1215



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.127    0.293 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 279,851
Number of extensions: 258
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)