Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004110                                                              17    2528
NP_001155155                                                           17    4312
NP_733764                                                              17    4312
NP_653273                                                              17    4312
NP_116012                                                              17    4312
NP_659465                                                              16    5631
NP_001273503                                                           16    5631
NP_001273505                                                           16    5631
NP_065833                                                              16    5631
XP_006715081                                                           16    5631
XP_005248977                                                           16    5631
XP_005248976                                                           16    5631
XP_006710232                                                           16    5631
NP_001076585                                                           16    7355
NP_005032                                                              16    7355
NP_055668                                                              16    7355
NP_005051                                                              16    7355
NP_001001523                                                           16    7355
XP_006721677                                                           16    7355
XP_006721676                                                           16    7355
XP_005245481                                                           16    7355
XP_006711547                                                           16    7355
NP_001091979                                                           16    7355
NP_079408                                                              15    9605
NP_008835                                                              15    9605
NP_001075109                                                           15    9605
XP_006714089                                                           15    9605
XP_006714088                                                           15    9605
XP_005262715                                                           15    9605

>NP_004110
          Length = 993

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VLAEASACQ 9
           VLAEASA Q
Sbjct: 441 VLAEASASQ 449



>NP_001155155
          Length = 1060

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LAEASAC 8
           LAEA+AC
Sbjct: 747 LAEANAC 753



>NP_733764
          Length = 815

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LAEASAC 8
           LAEA+AC
Sbjct: 502 LAEANAC 508



>NP_653273
          Length = 1139

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LAEASAC 8
           LAEA+AC
Sbjct: 826 LAEANAC 832



>NP_116012
          Length = 240

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLAEASAC 8
           V+ EASAC
Sbjct: 132 VIKEASAC 139



>NP_659465
          Length = 367

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AEASAC 8
           AEASAC
Sbjct: 129 AEASAC 134



>NP_001273503
          Length = 340

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AEASAC 8
           AEASAC
Sbjct: 102 AEASAC 107



>NP_001273505
          Length = 330

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AEASAC 8
           AEASAC
Sbjct: 102 AEASAC 107



>NP_065833
          Length = 505

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LAEASACQ 9
           LAE SAC+
Sbjct: 334 LAEFSACE 341



>XP_006715081
          Length = 253

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  AEASAC 8
          AEASAC
Sbjct: 15 AEASAC 20



>XP_005248977
          Length = 367

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AEASAC 8
           AEASAC
Sbjct: 129 AEASAC 134



>XP_005248976
          Length = 367

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AEASAC 8
           AEASAC
Sbjct: 129 AEASAC 134



>XP_006710232
          Length = 141

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  VLAEASAC 8
          V+AE++AC
Sbjct: 85 VMAESTAC 92



>NP_001076585
          Length = 555

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VLAEASACQ 9
           + AEA AC+
Sbjct: 272 ISAEAKACE 280



>NP_005032
          Length = 555

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VLAEASACQ 9
           + AEA AC+
Sbjct: 272 ISAEAKACE 280



>NP_055668
          Length = 1051

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLAEASACQ 9
           VLAE  AC+
Sbjct: 651 VLAEWKACE 659



>NP_005051
          Length = 518

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LAEASAC 8
           L EASAC
Sbjct: 166 LPEASAC 172



>NP_001001523
          Length = 497

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LAEASAC 8
           L EASAC
Sbjct: 145 LPEASAC 151



>XP_006721677
          Length = 1028

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLAEASACQ 9
           VLAE  AC+
Sbjct: 628 VLAEWKACE 636



>XP_006721676
          Length = 1073

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLAEASACQ 9
           VLAE  AC+
Sbjct: 673 VLAEWKACE 681



>XP_005245481
          Length = 512

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LAEASAC 8
           L EASAC
Sbjct: 160 LPEASAC 166



>XP_006711547
          Length = 572

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LAEASAC 8
           L EASAC
Sbjct: 220 LPEASAC 226



>NP_001091979
          Length = 1006

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLAEASACQ 9
           VLAE  AC+
Sbjct: 606 VLAEWKACE 614



>NP_079408
          Length = 1342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  VLAEASAC 8
          V+AE S+C
Sbjct: 71 VIAEKSSC 78



>NP_008835
          Length = 4128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    VLAEASAC 8
            +LA+ SAC
Sbjct: 3774 ILAQDSAC 3781



>NP_001075109
          Length = 4097

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    VLAEASAC 8
            +LA+ SAC
Sbjct: 3774 ILAQDSAC 3781



>XP_006714089
          Length = 1281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  VLAEASAC 8
          V+AE S+C
Sbjct: 71 VIAEKSSC 78



>XP_006714088
          Length = 1283

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  VLAEASAC 8
          V+AE S+C
Sbjct: 12 VIAEKSSC 19



>XP_005262715
          Length = 1320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  VLAEASAC 8
          V+AE S+C
Sbjct: 49 VIAEKSSC 56



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.114    0.311 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 400,467
Number of extensions: 323
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)