Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              19     665
XP_006718907                                                           19     665
XP_006718906                                                           19     665
XP_005271619                                                           19     665
XP_005271618                                                           19     665
NP_940686                                                              17    4312
NP_852091                                                              16    7355
NP_001124396                                                           16    7355
NP_001010866                                                           16    7355
NP_115830                                                              16    7355
NP_004188                                                              16    7355
NP_055646                                                              16    7355
XP_005268647                                                           16    7355
XP_005268646                                                           16    7355
XP_005268645                                                           16    7355
XP_006713495                                                           16    7355
XP_006710481                                                           16    7355
XP_006710480                                                           16    7355
XP_005270499                                                           16    7355
NP_008938                                                              16    7355
NP_003038                                                              15    9605
NP_001274359                                                           15    9605
NP_073613                                                              15    9605
NP_872409                                                              15    9605
NP_001274355                                                           15    9605
NP_001274358                                                           15    9605
NP_003355                                                              15    9605
NP_001274357                                                           15    9605
XP_006718595                                                           15    9605
XP_006717897                                                           15    9605
XP_006717896                                                           15    9605
XP_005249895                                                           15    9605
XP_005276058                                                           15    9605
>NP_000042
          Length = 3056

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VKYFIKRAN 9
           VKYFIK AN
Sbjct: 101 VKYFIKCAN 109
>XP_006718907
          Length = 3050

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VKYFIKRAN 9
           VKYFIK AN
Sbjct: 101 VKYFIKCAN 109
>XP_006718906
          Length = 3056

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VKYFIKRAN 9
           VKYFIK AN
Sbjct: 101 VKYFIKCAN 109
>XP_005271619
          Length = 3056

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VKYFIKRAN 9
           VKYFIK AN
Sbjct: 101 VKYFIKCAN 109
>XP_005271618
          Length = 3056

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VKYFIKRAN 9
           VKYFIK AN
Sbjct: 101 VKYFIKCAN 109
>NP_940686
          Length = 259

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KYFIKRAN 9
           KYFIK  N
Sbjct: 197 KYFIKSEN 204
>NP_852091
          Length = 941

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   YFIKRAN 9
           YFIK+A+
Sbjct: 513 YFIKKAH 519
>NP_001124396
          Length = 666

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VKYFIKRAN 9
           V+Y+IK  N
Sbjct: 171 VEYYIKHQN 179
>NP_001010866
          Length = 392

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VKYFIKRAN 9
           V+Y+IK  N
Sbjct: 171 VEYYIKHQN 179
>NP_115830
          Length = 368

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   VKYFIK 6
           +KYF+K
Sbjct: 299 IKYFVK 304
>NP_004188
          Length = 364

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   VKYFIK 6
           +KYF+K
Sbjct: 295 IKYFVK 300
>NP_055646
          Length = 2925

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    KYFIKR 7
            KYFI+R
Sbjct: 1669 KYFIRR 1674
>XP_005268647
          Length = 552

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VKYFIKRAN 9
           +K+F  RAN
Sbjct: 112 IKFFENRAN 120
>XP_005268646
          Length = 588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VKYFIKRAN 9
           +K+F  RAN
Sbjct: 112 IKFFENRAN 120
>XP_005268645
          Length = 589

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VKYFIKRAN 9
           +K+F  RAN
Sbjct: 112 IKFFENRAN 120
>XP_006713495
          Length = 2969

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    KYFIKR 7
            KYFI+R
Sbjct: 1713 KYFIRR 1718
>XP_006710481
          Length = 542

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VKYFIKRAN 9
           V+Y+IK  N
Sbjct: 171 VEYYIKHQN 179
>XP_006710480
          Length = 642

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VKYFIKRAN 9
           V+Y+IK  N
Sbjct: 171 VEYYIKHQN 179
>XP_005270499
          Length = 574

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KYFIKRAN 9
           K F+KR+N
Sbjct: 540 KSFVKRSN 547
>NP_008938
          Length = 551

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VKYFIKRAN 9
           +K+F  RAN
Sbjct: 112 IKFFENRAN 120
>NP_003038
          Length = 815

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   VKYFIK 6
           +KYF+K
Sbjct: 379 IKYFLK 384
>NP_001274359
          Length = 173

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VKYFIKR 7
           V YFIK+
Sbjct: 162 VSYFIKK 168
>NP_073613
          Length = 747

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KYFIKRAN 9
           KYFIK  +
Sbjct: 721 KYFIKEGD 728
>NP_872409
          Length = 389

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VKYFIK 6
           VKYFI+
Sbjct: 341 VKYFIR 346
>NP_001274355
          Length = 310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VKYFIKR 7
           V YFIK+
Sbjct: 299 VSYFIKK 305
>NP_001274358
          Length = 173

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VKYFIKR 7
           V YFIK+
Sbjct: 162 VSYFIKK 168
>NP_003355
          Length = 310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VKYFIKR 7
           V YFIK+
Sbjct: 299 VSYFIKK 305
>NP_001274357
          Length = 173

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VKYFIKR 7
           V YFIK+
Sbjct: 162 VSYFIKK 168
>XP_006718595
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VKYFIK 6
           VKYFI+
Sbjct: 341 VKYFIR 346
>XP_006717897
          Length = 1160

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VKYFIK 6
           +KYFIK
Sbjct: 224 LKYFIK 229
>XP_006717896
          Length = 1186

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VKYFIK 6
           +KYFIK
Sbjct: 250 LKYFIK 255
>XP_005249895
          Length = 246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VKYFIKR 7
           V YFIK+
Sbjct: 235 VSYFIKK 241
>XP_005276058
          Length = 147

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   VKYFIKRAN 9
           + +F+KR N
Sbjct: 137 ILFFLKRGN 145
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.335    0.144    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 404,242
Number of extensions: 504
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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