Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              21     229
XP_006716142                                                           21     229
XP_005250088                                                           21     229
XP_006716141                                                           21     229
XP_006716140                                                           21     229
XP_005250084                                                           21     229
XP_005250083                                                           21     229
XP_005250082                                                           21     229
NP_005874                                                              17    3301
XP_006722673                                                           17    3301
XP_006722672                                                           17    3301
XP_006722671                                                           17    3301
XP_006722670                                                           17    3301
XP_005259532                                                           17    3301
NP_001010873                                                           17    4312
NP_001153198                                                           17    4312
NP_803875                                                              17    4312
NP_055498                                                              16    5631
NP_001136082                                                           16    5631
NP_065939                                                              16    5631
NP_001185901                                                           16    5631
XP_006721669                                                           16    5631
XP_006710855                                                           16    5631
XP_006710854                                                           16    5631
NP_073744                                                              16    7355
NP_001030611                                                           16    7355
NP_001361                                                              16    7355
NP_443152                                                              16    7355
XP_005252192                                                           16    7355
XP_006712019                                                           16    7355
XP_006711992                                                           16    7355
NP_004499                                                              16    7355
NP_001073896                                                           15    9605
NP_003253                                                              15    9605
NP_001244289                                                           15    9605
NP_004332                                                              15    9605
NP_981949                                                              15    9605
NP_631895                                                              15    9605
NP_001244290                                                           15    9605
NP_004028                                                              15    9605
NP_689760                                                              15    9605
XP_006724219                                                           15    9605
XP_006724218                                                           15    9605
XP_006724217                                                           15    9605
XP_006714888                                                           15    9605
XP_006714887                                                           15    9605
XP_006714886                                                           15    9605
XP_005263859                                                           15    9605
XP_005264615                                                           15    9605
XP_006712164                                                           15    9605
XP_005264614                                                           15    9605
XP_005264613                                                           15    9605
XP_005264612                                                           15    9605
XP_006711542                                                           15    9605
XP_006711541                                                           15    9605
XP_005245460                                                           15    9605
XP_005270809                                                           15    9605
NP_689726                                                              15    9605
>NP_733751
          Length = 4911

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VKRFVLMPH 9
            VKRFVL PH
Sbjct: 4719 VKRFVLRPH 4727
>XP_006716142
          Length = 4927

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VKRFVLMPH 9
            VKRFVL PH
Sbjct: 4735 VKRFVLRPH 4743
>XP_005250088
          Length = 4928

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VKRFVLMPH 9
            VKRFVL PH
Sbjct: 4736 VKRFVLRPH 4744
>XP_006716141
          Length = 4959

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VKRFVLMPH 9
            VKRFVL PH
Sbjct: 4767 VKRFVLRPH 4775
>XP_006716140
          Length = 4982

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VKRFVLMPH 9
            VKRFVL PH
Sbjct: 4790 VKRFVLRPH 4798
>XP_005250084
          Length = 4982

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VKRFVLMPH 9
            VKRFVL PH
Sbjct: 4790 VKRFVLRPH 4798
>XP_005250083
          Length = 4982

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VKRFVLMPH 9
            VKRFVL PH
Sbjct: 4790 VKRFVLRPH 4798
>XP_005250082
          Length = 4983

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VKRFVLMPH 9
            VKRFVL PH
Sbjct: 4791 VKRFVLRPH 4799
>NP_005874
          Length = 2303

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VKRFVLMPH 9
            VKR+  +PH
Sbjct: 2096 VKRYASLPH 2104
>XP_006722673
          Length = 2302

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VKRFVLMPH 9
            VKR+  +PH
Sbjct: 2095 VKRYASLPH 2103
>XP_006722672
          Length = 2303

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VKRFVLMPH 9
            VKR+  +PH
Sbjct: 2096 VKRYASLPH 2104
>XP_006722671
          Length = 2303

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VKRFVLMPH 9
            VKR+  +PH
Sbjct: 2096 VKRYASLPH 2104
>XP_006722670
          Length = 2326

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VKRFVLMPH 9
            VKR+  +PH
Sbjct: 2119 VKRYASLPH 2127
>XP_005259532
          Length = 2327

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VKRFVLMPH 9
            VKR+  +PH
Sbjct: 2120 VKRYASLPH 2128
>NP_001010873
          Length = 170

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  FVLMPH 9
          FVL+PH
Sbjct: 8  FVLLPH 13
>NP_001153198
          Length = 170

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  FVLMPH 9
          FVL+PH
Sbjct: 8  FVLLPH 13
>NP_803875
          Length = 4243

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    KRFVLMPH 9
            +RFVL+P+
Sbjct: 1653 RRFVLLPN 1660
>NP_055498
          Length = 1036

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FVLMPH 9
           FVL+PH
Sbjct: 353 FVLVPH 358
>NP_001136082
          Length = 1036

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FVLMPH 9
           FVL+PH
Sbjct: 353 FVLVPH 358
>NP_065939
          Length = 1094

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RFVLMPH 9
           RFV+ PH
Sbjct: 657 RFVIPPH 663
>NP_001185901
          Length = 1035

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RFVLMPH 9
           RFV+ PH
Sbjct: 598 RFVIPPH 604
>XP_006721669
          Length = 1009

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FVLMPH 9
           FVL+PH
Sbjct: 353 FVLVPH 358
>XP_006710855
          Length = 821

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RFVLMPH 9
           RFV+ PH
Sbjct: 384 RFVIPPH 390
>XP_006710854
          Length = 1046

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RFVLMPH 9
           RFV+ PH
Sbjct: 609 RFVIPPH 615
>NP_073744
          Length = 746

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  RFVLMPH 9
          RF L+PH
Sbjct: 88 RFSLVPH 94
>NP_001030611
          Length = 733

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  RFVLMPH 9
          RF L+PH
Sbjct: 75 RFSLVPH 81
>NP_001361
          Length = 4158

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4    FVLMPH 9
            FV++PH
Sbjct: 1037 FVILPH 1042
>NP_443152
          Length = 875

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   KRFVLMPH 9
           KR+ ++PH
Sbjct: 557 KRYHMLPH 564
>XP_005252192
          Length = 735

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  RFVLMPH 9
          RF L+PH
Sbjct: 77 RFSLVPH 83
>XP_006712019
          Length = 4158

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4    FVLMPH 9
            FV++PH
Sbjct: 1037 FVILPH 1042
>XP_006711992
          Length = 875

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   KRFVLMPH 9
           KR+ ++PH
Sbjct: 557 KRYHMLPH 564
>NP_004499
          Length = 284

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  VKRFVLMP 8
          ++ FV+MP
Sbjct: 52 IRHFVMMP 59
>NP_001073896
          Length = 1577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3    RFVLMP 8
            RF++MP
Sbjct: 1244 RFIIMP 1249
>NP_003253
          Length = 399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KRFVLMPH 9
           K+F L PH
Sbjct: 166 KKFKLEPH 173
>NP_001244289
          Length = 879

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FVLMPH 9
           FVL PH
Sbjct: 682 FVLRPH 687
>NP_004332
          Length = 2225

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3    RFVLMPH 9
            +F+L PH
Sbjct: 2164 QFILTPH 2170
>NP_981949
          Length = 760

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FVLMPH 9
           FVL PH
Sbjct: 563 FVLRPH 568
>NP_631895
          Length = 798

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FVLMPH 9
           FVL PH
Sbjct: 601 FVLRPH 606
>NP_001244290
          Length = 761

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FVLMPH 9
           FVL PH
Sbjct: 564 FVLRPH 569
>NP_004028
          Length = 879

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FVLMPH 9
           FVL PH
Sbjct: 682 FVLRPH 687
>NP_689760
          Length = 535

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FVLMPH 9
           FVL PH
Sbjct: 431 FVLAPH 436
>XP_006724219
          Length = 1157

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   FVLMPH 9
           FV++PH
Sbjct: 466 FVIVPH 471
>XP_006724218
          Length = 1200

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   FVLMPH 9
           FV++PH
Sbjct: 544 FVIVPH 549
>XP_006724217
          Length = 1235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   FVLMPH 9
           FV++PH
Sbjct: 544 FVIVPH 549
>XP_006714888
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FVLMPH 9
           FVL PH
Sbjct: 362 FVLAPH 367
>XP_006714887
          Length = 528

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FVLMPH 9
           FVL PH
Sbjct: 424 FVLAPH 429
>XP_006714886
          Length = 528

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FVLMPH 9
           FVL PH
Sbjct: 424 FVLAPH 429
>XP_005263859
          Length = 1606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3    RFVLMP 8
            RF++MP
Sbjct: 1273 RFIIMP 1278
>XP_005264615
          Length = 2162

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3    RFVLMPH 9
            +F+L PH
Sbjct: 2101 QFILTPH 2107
>XP_006712164
          Length = 2179

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3    RFVLMPH 9
            +F+L PH
Sbjct: 2118 QFILTPH 2124
>XP_005264614
          Length = 2191

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3    RFVLMPH 9
            +F+L PH
Sbjct: 2130 QFILTPH 2136
>XP_005264613
          Length = 2208

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3    RFVLMPH 9
            +F+L PH
Sbjct: 2147 QFILTPH 2153
>XP_005264612
          Length = 2242

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3    RFVLMPH 9
            +F+L PH
Sbjct: 2181 QFILTPH 2187
>XP_006711542
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  FVLMPH 9
          FVL PH
Sbjct: 31 FVLSPH 36
>XP_006711541
          Length = 509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  FVLMPH 9
          FVL PH
Sbjct: 31 FVLSPH 36
>XP_005245460
          Length = 606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  FVLMPH 9
          FVL PH
Sbjct: 31 FVLSPH 36
>XP_005270809
          Length = 804

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FVLMPH 9
           FVL PH
Sbjct: 607 FVLRPH 612
>NP_689726
          Length = 1274

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   FVLMPH 9
           FV++PH
Sbjct: 583 FVIVPH 588
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.345    0.152    0.499 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 457,147
Number of extensions: 702
Number of successful extensions: 58
Number of sequences better than 10000.0: 58
Number of HSP's gapped: 58
Number of HSP's successfully gapped: 58
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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