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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_071741                                                              16    7355
XP_006714035                                                           16    7355
XP_006714034                                                           16    7355
NP_001129666                                                           15    9605
NP_001129665                                                           15    9605
NP_665857                                                              15    9605
NP_001020461                                                           15    9605
NP_055744                                                              15    9605

>NP_071741
          Length = 1015

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VKLEVKALE 9
           +K E+KALE
Sbjct: 521 LKEEIKALE 529



>XP_006714035
          Length = 941

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VKLEVKALE 9
           +K E+KALE
Sbjct: 521 LKEEIKALE 529



>XP_006714034
          Length = 941

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VKLEVKALE 9
           +K E+KALE
Sbjct: 521 LKEEIKALE 529



>NP_001129666
          Length = 710

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KLEVKAL 8
           KLE+KAL
Sbjct: 328 KLEMKAL 334



>NP_001129665
          Length = 710

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KLEVKAL 8
           KLE+KAL
Sbjct: 328 KLEMKAL 334



>NP_665857
          Length = 349

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  VKLEVKA 7
          VKL+VKA
Sbjct: 33 VKLQVKA 39



>NP_001020461
          Length = 116

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  LEVKALE 9
          LE+KALE
Sbjct: 55 LELKALE 61



>NP_055744
          Length = 710

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KLEVKAL 8
           KLE+KAL
Sbjct: 328 KLEMKAL 334



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.129    0.290 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 323,081
Number of extensions: 380
Number of successful extensions: 8
Number of sequences better than 10000.0: 8
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)