Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_065681                                                              21     175
NP_066124                                                              21     175
XP_006717999                                                           21     175
NP_003204                                                              16    5631
NP_958851                                                              16    5631
NP_922932                                                              16    5631
NP_065102                                                              16    7355
XP_005266067                                                           16    7355
NP_689490                                                              16    7355
NP_068780                                                              15    9605
NP_109592                                                              15    9605
NP_002839                                                              15    9605
XP_006713374                                                           15    9605

>NP_065681
          Length = 1072

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VKATAFHMK 9
           VKATAFH+K
Sbjct: 739 VKATAFHLK 747



>NP_066124
          Length = 1114

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VKATAFHMK 9
           VKATAFH+K
Sbjct: 739 VKATAFHLK 747



>XP_006717999
          Length = 1207

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VKATAFHMK 9
           VKATAFH+K
Sbjct: 832 VKATAFHLK 840



>NP_003204
          Length = 434

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VKATAFH 7
           + ATAFH
Sbjct: 139 ISATAFH 145



>NP_958851
          Length = 305

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  VKATAFH 7
          + ATAFH
Sbjct: 10 ISATAFH 16



>NP_922932
          Length = 1117

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   VKATAFHMK 9
           V  TAFH K
Sbjct: 782 VAGTAFHYK 790



>NP_065102
          Length = 498

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TAFHMK 9
           TAFHM+
Sbjct: 213 TAFHMQ 218



>XP_005266067
          Length = 190

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   TAFHMK 9
           T FHMK
Sbjct: 129 TVFHMK 134



>NP_689490
          Length = 234

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   TAFHMK 9
           T FHMK
Sbjct: 173 TVFHMK 178



>NP_068780
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   VKATAFHMK 9
           V ATA H K
Sbjct: 131 VSATAIHNK 139



>NP_109592
          Length = 1216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  ATAFHM 8
          ATAFH+
Sbjct: 27 ATAFHV 32



>NP_002839
          Length = 1188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  ATAFHM 8
          ATAFH+
Sbjct: 27 ATAFHV 32



>XP_006713374
          Length = 511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KATAFHMK 9
           KAT +H K
Sbjct: 454 KATGWHFK 461



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.332    0.124    0.356 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 393,297
Number of extensions: 261
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)