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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_644814                                                              17    2528
XP_006721859                                                           17    2528
NP_006280                                                              16    5631
XP_006716915                                                           16    5631
XP_005251621                                                           16    5631
NP_689733                                                              15    9605
NP_001106869                                                           15    9605
NP_001106868                                                           15    9605
NP_001269654                                                           15    9605
NP_115915                                                              15    9605
NP_892018                                                              15    9605
XP_005259608                                                           15    9605
XP_006712387                                                           15    9605
XP_006712386                                                           15    9605
XP_006712385                                                           15    9605
XP_006712384                                                           15    9605

>NP_644814
          Length = 880

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   VKALEATTK 9
           ++A+EATTK
Sbjct: 440 LRAIEATTK 448



>XP_006721859
          Length = 844

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   VKALEATTK 9
           ++A+EATTK
Sbjct: 404 LRAIEATTK 412



>NP_006280
          Length = 2541

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    KALEATTK 9
            +ALEATT+
Sbjct: 2144 RALEATTE 2151



>XP_006716915
          Length = 2542

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    KALEATTK 9
            +ALEATT+
Sbjct: 2144 RALEATTE 2151



>XP_005251621
          Length = 2542

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    KALEATTK 9
            +ALEATT+
Sbjct: 2144 RALEATTE 2151



>NP_689733
          Length = 471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VKALEAT 7
           VKAL+AT
Sbjct: 185 VKALDAT 191



>NP_001106869
          Length = 369

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  VKALEAT 7
          VKAL+AT
Sbjct: 83 VKALDAT 89



>NP_001106868
          Length = 395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VKALEAT 7
           VKAL+AT
Sbjct: 109 VKALDAT 115



>NP_001269654
          Length = 369

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  VKALEAT 7
          VKAL+AT
Sbjct: 83 VKALDAT 89



>NP_115915
          Length = 368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VKALEATTK 9
           VKALEA  +
Sbjct: 100 VKALEAVNR 108



>NP_892018
          Length = 1059

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LEATTK 9
           LEATTK
Sbjct: 432 LEATTK 437



>XP_005259608
          Length = 1093

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LEATTK 9
           LEATTK
Sbjct: 466 LEATTK 471



>XP_006712387
          Length = 382

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VKALEAT 7
           VKAL+AT
Sbjct: 96  VKALDAT 102



>XP_006712386
          Length = 382

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VKALEAT 7
           VKAL+AT
Sbjct: 96  VKALDAT 102



>XP_006712385
          Length = 382

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VKALEAT 7
           VKAL+AT
Sbjct: 96  VKALDAT 102



>XP_006712384
          Length = 382

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VKALEAT 7
           VKAL+AT
Sbjct: 96  VKALDAT 102



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.298    0.108    0.243 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 234,472
Number of extensions: 125
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)