Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     299
XP_005262424                                                           20     299
NP_110517                                                              17    3301
NP_001268424                                                           17    3301
NP_055758                                                              17    4312
NP_071427                                                              17    4312
XP_005262232                                                           17    4312
XP_005250893                                                           17    4312
XP_005250894                                                           17    4312
NP_060048                                                              16    5631
NP_001191102                                                           16    5631
NP_003038                                                              16    5631
NP_733827                                                              16    5631
NP_001028750                                                           16    5631
NP_037389                                                              16    5631
XP_005247608                                                           16    5631
XP_005247606                                                           16    5631
XP_005247195                                                           16    5631
XP_006713576                                                           16    5631
XP_005247194                                                           16    5631
NP_001012337                                                           16    5631
YP_003024027                                                           16    5631
NP_001001344                                                           16    7355
NP_001268231                                                           16    7355
NP_115756                                                              16    7355
NP_733792                                                              16    7355
NP_733781                                                              16    7355
NP_038464                                                              16    7355
NP_995314                                                              16    7355
NP_060101                                                              16    7355
NP_689786                                                              16    7355
NP_997273                                                              16    7355
NP_060127                                                              16    7355
XP_005274748                                                           16    7355
XP_005274747                                                           16    7355
XP_006724886                                                           16    7355
XP_005274746                                                           16    7355
XP_005251788                                                           16    7355
XP_005251787                                                           16    7355
XP_006714784                                                           16    7355
XP_006712264                                                           16    7355
XP_006712263                                                           16    7355
XP_005264437                                                           16    7355
NP_982261                                                              16    7355
NP_001002243                                                           16    7355
NP_001191762                                                           15    9605
NP_001191763                                                           15    9605
NP_001191760                                                           15    9605
NP_060545                                                              15    9605
NP_001191761                                                           15    9605
NP_003238                                                              15    9605
NP_001094058                                                           15    9605
NP_060009                                                              15    9605
NP_061323                                                              15    9605
NP_056284                                                              15    9605
XP_006721125                                                           15    9605
XP_005265322                                                           15    9605
NP_055040                                                              15    9605
NP_803875                                                              15    9605
>NP_722576
          Length = 3080

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    VINEFTEN 8
            VINEFTEN
Sbjct: 2266 VINEFTEN 2273
>XP_005262424
          Length = 2875

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    VINEFTEN 8
            VINEFTEN
Sbjct: 2061 VINEFTEN 2068
>NP_110517
          Length = 563

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VINEFTEN 8
           ++N+FTEN
Sbjct: 426 LLNKFTEN 433
>NP_001268424
          Length = 536

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VINEFTEN 8
           ++N+FTEN
Sbjct: 399 LLNKFTEN 406
>NP_055758
          Length = 837

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  INEFTENL 9
          +NEFTE++
Sbjct: 90 LNEFTEHV 97
>NP_071427
          Length = 317

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   INEFTEN 8
           IN++TEN
Sbjct: 245 INDYTEN 251
>XP_005262232
          Length = 254

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   INEFTEN 8
           IN++TEN
Sbjct: 182 INDYTEN 188
>XP_005250893
          Length = 837

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  INEFTENL 9
          +NEFTE++
Sbjct: 90 LNEFTEHV 97
>XP_005250894
          Length = 837

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  INEFTENL 9
          +NEFTE++
Sbjct: 90 LNEFTEHV 97
>NP_060048
          Length = 212

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   INEFTEN 8
           +N+FT+N
Sbjct: 200 VNDFTQN 206
>NP_001191102
          Length = 496

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   INEFTENL 9
           +NEF +NL
Sbjct: 94  LNEFIQNL 101
>NP_003038
          Length = 815

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   INEFTENL 9
           + +FTENL
Sbjct: 179 LRQFTENL 186
>NP_733827
          Length = 464

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   INEFTENL 9
           +NEF +NL
Sbjct: 94  LNEFIQNL 101
>NP_001028750
          Length = 496

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   INEFTENL 9
           +NEF +NL
Sbjct: 94  LNEFIQNL 101
>NP_037389
          Length = 496

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   INEFTENL 9
           +NEF +NL
Sbjct: 94  LNEFIQNL 101
>XP_005247608
          Length = 120

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   INEFTEN 8
           +N+FT+N
Sbjct: 108 VNDFTQN 114
>XP_005247606
          Length = 212

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   INEFTEN 8
           +N+FT+N
Sbjct: 200 VNDFTQN 206
>XP_005247195
          Length = 120

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   INEFTEN 8
           +N+FT+N
Sbjct: 108 VNDFTQN 114
>XP_006713576
          Length = 212

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   INEFTEN 8
           +N+FT+N
Sbjct: 200 VNDFTQN 206
>XP_005247194
          Length = 212

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   INEFTEN 8
           +N+FT+N
Sbjct: 200 VNDFTQN 206
>NP_001012337
          Length = 212

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   INEFTEN 8
           +N+FT+N
Sbjct: 200 VNDFTQN 206
>YP_003024027
          Length = 347

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VINEFTEN 8
           +I EFT+N
Sbjct: 266 IIEEFTKN 273
>NP_001001344
          Length = 1220

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    VINEFTENL 9
            +IN++T N+
Sbjct: 1148 LINDYTHNI 1156
>NP_001268231
          Length = 811

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VINEFTEN 8
           V+ EF+EN
Sbjct: 327 VLEEFSEN 334
>NP_115756
          Length = 779

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VINEFTEN 8
           V+ EF+EN
Sbjct: 295 VLEEFSEN 302
>NP_733792
          Length = 732

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VINEFTEN 8
           V+ EF+EN
Sbjct: 295 VLEEFSEN 302
>NP_733781
          Length = 513

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VINEFTEN 8
           V+ EF+EN
Sbjct: 295 VLEEFSEN 302
>NP_038464
          Length = 1128

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VINEFTENL 9
           V+NE  +NL
Sbjct: 441 VLNELVQNL 449
>NP_995314
          Length = 1134

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VINEFTENL 9
           V+NE  +NL
Sbjct: 447 VLNELVQNL 455
>NP_060101
          Length = 1712

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VINEFTENL 9
           V N FT+NL
Sbjct: 828 VQNRFTKNL 836
>NP_689786
          Length = 740

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NEFTENL 9
           NEF EN+
Sbjct: 564 NEFRENI 570
>NP_997273
          Length = 306

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VINEFTENL 9
           +IN F E+L
Sbjct: 291 IINSFAEHL 299
>NP_060127
          Length = 909

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  INEFTENL 9
          I+EF+EN+
Sbjct: 68 IHEFSENV 75
>XP_005274748
          Length = 1206

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    VINEFTENL 9
            +IN++T N+
Sbjct: 1134 LINDYTHNI 1142
>XP_005274747
          Length = 1220

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    VINEFTENL 9
            +IN++T N+
Sbjct: 1148 LINDYTHNI 1156
>XP_006724886
          Length = 1235

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    VINEFTENL 9
            +IN++T N+
Sbjct: 1163 LINDYTHNI 1171
>XP_005274746
          Length = 1249

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    VINEFTENL 9
            +IN++T N+
Sbjct: 1177 LINDYTHNI 1185
>XP_005251788
          Length = 611

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NEFTENL 9
           NEF EN+
Sbjct: 435 NEFRENI 441
>XP_005251787
          Length = 717

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NEFTENL 9
           NEF EN+
Sbjct: 541 NEFRENI 547
>XP_006714784
          Length = 734

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VINEFTEN 8
           V+ EF+EN
Sbjct: 295 VLEEFSEN 302
>XP_006712264
          Length = 1126

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VINEFTENL 9
           V+NE  +NL
Sbjct: 439 VLNELVQNL 447
>XP_006712263
          Length = 1132

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VINEFTENL 9
           V+NE  +NL
Sbjct: 445 VLNELVQNL 453
>XP_005264437
          Length = 937

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  INEFTENL 9
          I+EF+EN+
Sbjct: 68 IHEFSENV 75
>NP_982261
          Length = 936

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  INEFTENL 9
          I+EF+EN+
Sbjct: 68 IHEFSENV 75
>NP_001002243
          Length = 908

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  INEFTENL 9
          I+EF+EN+
Sbjct: 68 IHEFSENV 75
>NP_001191762
          Length = 549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VINEFTE 7
           V+N FTE
Sbjct: 412 VLNNFTE 418
>NP_001191763
          Length = 470

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VINEFTE 7
           V+N FTE
Sbjct: 333 VLNNFTE 339
>NP_001191760
          Length = 627

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VINEFTE 7
           V+N FTE
Sbjct: 523 VLNNFTE 529
>NP_060545
          Length = 660

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VINEFTE 7
           V+N FTE
Sbjct: 523 VLNNFTE 529
>NP_001191761
          Length = 594

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VINEFTE 7
           V+N FTE
Sbjct: 457 VLNNFTE 463
>NP_003238
          Length = 1172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VINEFTENL 9
           ++N+ +ENL
Sbjct: 286 LVNQLSENL 294
>NP_001094058
          Length = 1191

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  NEFTENL 9
          NEF EN+
Sbjct: 19 NEFDENV 25
>NP_060009
          Length = 4116

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    EFTENL 9
            EFT+NL
Sbjct: 1210 EFTDNL 1215
>NP_061323
          Length = 1064

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  NEFTENL 9
          NEF EN+
Sbjct: 19 NEFDENV 25
>NP_056284
          Length = 247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  INEFTEN 8
          +NEF EN
Sbjct: 49 MNEFLEN 55
>XP_006721125
          Length = 4070

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    EFTENL 9
            EFT+NL
Sbjct: 1164 EFTDNL 1169
>XP_005265322
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VINEFTE 7
           V+N FTE
Sbjct: 523 VLNNFTE 529
>NP_055040
          Length = 589

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   INEFTENL 9
           + EF ENL
Sbjct: 306 VKEFCENL 313
>NP_803875
          Length = 4243

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1    VINEFTENL 9
            ++  FTEN+
Sbjct: 3281 LVGSFTENM 3289
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.136    0.352 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 512,998
Number of extensions: 1141
Number of successful extensions: 59
Number of sequences better than 10000.0: 59
Number of HSP's gapped: 59
Number of HSP's successfully gapped: 59
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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