Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000213                                                              20     299
NP_001087241                                                           20     299
XP_005265799                                                           20     299
XP_005265798                                                           20     299
XP_005265797                                                           20     299
NP_001073343                                                           17    3301
NP_006104                                                              17    3301
XP_005270417                                                           17    3301
XP_005270416                                                           17    3301
NP_001273590                                                           17    4312
NP_001273586                                                           17    4312
NP_001130010                                                           17    4312
XP_006716482                                                           17    4312
XP_005251207                                                           17    4312
XP_006715794                                                           17    4312
XP_006715793                                                           17    4312
NP_001004320                                                           17    4312
NP_079523                                                              17    4312
NP_005906                                                              16    5631
NP_036499                                                              16    5631
NP_001122074                                                           16    7355
NP_005730                                                              16    7355
NP_001244950                                                           16    7355
NP_001244948                                                           16    7355
NP_001244949                                                           16    7355
NP_001244951                                                           16    7355
NP_001244952                                                           16    7355
NP_000818                                                              16    7355
NP_001107655                                                           16    7355
NP_002734                                                              16    7355
NP_002800                                                              16    7355
NP_001036038                                                           16    7355
XP_006722861                                                           16    7355
XP_006722860                                                           16    7355
XP_006722859                                                           16    7355
XP_006722858                                                           16    7355
XP_006722081                                                           16    7355
XP_006722080                                                           16    7355
XP_006722079                                                           16    7355
XP_006722078                                                           16    7355
XP_005254171                                                           16    7355
XP_006718884                                                           16    7355
XP_006718882                                                           16    7355
NP_001276033                                                           16    7355
NP_001001329                                                           16    7355
NP_001276031                                                           16    7355
NP_001276032                                                           16    7355
NP_006197                                                              15    9605
NP_002600                                                              15    9605
NP_003451                                                              15    9605
NP_002963                                                              15    9605
NP_001277033                                                           15    9605
NP_775876                                                              15    9605
NP_057529                                                              15    9605
NP_031394                                                              15    9605
NP_003319                                                              15    9605
XP_005261992                                                           15    9605
XP_005261991                                                           15    9605
XP_005261989                                                           15    9605
XP_005261988                                                           15    9605
XP_005255619                                                           15    9605
XP_005266253                                                           15    9605
XP_005268521                                                           15    9605
XP_005265800                                                           15    9605
XP_006714103                                                           15    9605
XP_006714102                                                           15    9605
NP_001099040                                                           15    9605
>NP_000213
          Length = 976

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VIKEGFRML 9
           +IKEGFRML
Sbjct: 882 MIKEGFRML 890
>NP_001087241
          Length = 972

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VIKEGFRML 9
           +IKEGFRML
Sbjct: 878 MIKEGFRML 886
>XP_005265799
          Length = 973

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VIKEGFRML 9
           +IKEGFRML
Sbjct: 879 MIKEGFRML 887
>XP_005265798
          Length = 976

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VIKEGFRML 9
           +IKEGFRML
Sbjct: 882 MIKEGFRML 890
>XP_005265797
          Length = 977

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VIKEGFRML 9
           +IKEGFRML
Sbjct: 883 MIKEGFRML 891
>NP_001073343
          Length = 287

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IKEGFRML 9
           +KEGFR L
Sbjct: 191 LKEGFRTL 198
>NP_006104
          Length = 847

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IKEGFRML 9
           +KEGFR L
Sbjct: 751 LKEGFRTL 758
>XP_005270417
          Length = 753

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IKEGFRML 9
           +KEGFR L
Sbjct: 657 LKEGFRTL 664
>XP_005270416
          Length = 805

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IKEGFRML 9
           +KEGFR L
Sbjct: 751 LKEGFRTL 758
>NP_001273590
          Length = 144

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IKEGFRML 9
           I+EGFR L
Sbjct: 125 IREGFRSL 132
>NP_001273586
          Length = 324

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IKEGFRML 9
           I+EGFR L
Sbjct: 239 IREGFRSL 246
>NP_001130010
          Length = 1090

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IKEGFRM 8
           +KEGFR+
Sbjct: 629 VKEGFRV 635
>XP_006716482
          Length = 324

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IKEGFRML 9
           I+EGFR L
Sbjct: 239 IREGFRSL 246
>XP_005251207
          Length = 324

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IKEGFRML 9
           I+EGFR L
Sbjct: 239 IREGFRSL 246
>XP_006715794
          Length = 423

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  IKEGFRML 9
          + +GFRML
Sbjct: 12 VSQGFRML 19
>XP_006715793
          Length = 456

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  IKEGFRML 9
          + +GFRML
Sbjct: 12 VSQGFRML 19
>NP_001004320
          Length = 445

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  IKEGFRML 9
          + +GFRML
Sbjct: 12 VSQGFRML 19
>NP_079523
          Length = 1059

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IKEGFRM 8
           +KEGFR+
Sbjct: 598 VKEGFRV 604
>NP_005906
          Length = 821

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IKEGFRML 9
           +KE FR+L
Sbjct: 645 VKEAFRLL 652
>NP_036499
          Length = 313

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IKEGFRML 9
           +KEGF+ L
Sbjct: 297 VKEGFKRL 304
>NP_001122074
          Length = 762

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VIKEGFR 7
           VIK+G+R
Sbjct: 530 VIKQGYR 536
>NP_005730
          Length = 797

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VIKEGFR 7
           VIK+G+R
Sbjct: 565 VIKQGYR 571
>NP_001244950
          Length = 916

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEGFRML 9
           +EG+RML
Sbjct: 192 EEGYRML 198
>NP_001244948
          Length = 826

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEGFRML 9
           +EG+RML
Sbjct: 102 EEGYRML 108
>NP_001244949
          Length = 811

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  KEGFRML 9
          +EG+RML
Sbjct: 87 EEGYRML 93
>NP_001244951
          Length = 916

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEGFRML 9
           +EG+RML
Sbjct: 192 EEGYRML 198
>NP_001244952
          Length = 837

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEGFRML 9
           +EG+RML
Sbjct: 113 EEGYRML 119
>NP_000818
          Length = 906

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEGFRML 9
           +EG+RML
Sbjct: 182 EEGYRML 188
>NP_001107655
          Length = 906

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KEGFRML 9
           +EG+RML
Sbjct: 182 EEGYRML 188
>NP_002734
          Length = 528

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VIKEGFRM 8
           V +EGFR+
Sbjct: 133 VTREGFRL 140
>NP_002800
          Length = 534

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VIKEGFRML 9
           VIK G RM+
Sbjct: 402 VIKTGVRMI 410
>NP_001036038
          Length = 743

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VIKEGFRM 8
           V KEGFR+
Sbjct: 720 VPKEGFRV 727
>XP_006722861
          Length = 533

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VIKEGFRM 8
           V +EGFR+
Sbjct: 133 VTREGFRL 140
>XP_006722860
          Length = 536

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VIKEGFRM 8
           V +EGFR+
Sbjct: 133 VTREGFRL 140
>XP_006722859
          Length = 543

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VIKEGFRM 8
           V +EGFR+
Sbjct: 133 VTREGFRL 140
>XP_006722858
          Length = 543

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VIKEGFRM 8
           V +EGFR+
Sbjct: 133 VTREGFRL 140
>XP_006722081
          Length = 449

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VIKEGFRM 8
           V KEGFR+
Sbjct: 426 VPKEGFRV 433
>XP_006722080
          Length = 557

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VIKEGFRM 8
           V KEGFR+
Sbjct: 534 VPKEGFRV 541
>XP_006722079
          Length = 613

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VIKEGFRM 8
           V KEGFR+
Sbjct: 590 VPKEGFRV 597
>XP_006722078
          Length = 613

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VIKEGFRM 8
           V KEGFR+
Sbjct: 590 VPKEGFRV 597
>XP_005254171
          Length = 746

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VIKEGFR 7
           VIK+G+R
Sbjct: 565 VIKQGYR 571
>XP_006718884
          Length = 267

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEGFRM 8
           KEGFR+
Sbjct: 115 KEGFRL 120
>XP_006718882
          Length = 182

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEGFRM 8
           KEGFR+
Sbjct: 115 KEGFRL 120
>NP_001276033
          Length = 535

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VIKEGFRM 8
           V +EGFR+
Sbjct: 133 VTREGFRL 140
>NP_001001329
          Length = 525

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VIKEGFRM 8
           V +EGFR+
Sbjct: 133 VTREGFRL 140
>NP_001276031
          Length = 525

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VIKEGFRM 8
           V +EGFR+
Sbjct: 133 VTREGFRL 140
>NP_001276032
          Length = 535

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VIKEGFRM 8
           V +EGFR+
Sbjct: 133 VTREGFRL 140
>NP_006197
          Length = 1089

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IKEGFRM 8
           IK G+RM
Sbjct: 909 IKSGYRM 915
>NP_002600
          Length = 1106

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IKEGFRM 8
           IK G+RM
Sbjct: 917 IKRGYRM 923
>NP_003451
          Length = 745

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IKEGFRML 9
           +KEGF +L
Sbjct: 203 MKEGFSLL 210
>NP_002963
          Length = 1893

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    EGFRML 9
            EGFR+L
Sbjct: 1473 EGFRLL 1478
>NP_001277033
          Length = 736

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IKEGFRML 9
           +KEGF +L
Sbjct: 203 MKEGFSLL 210
>NP_775876
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VIKEGFRML 9
           ++KEGF ++
Sbjct: 218 LLKEGFHLV 226
>NP_057529
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  VIKEGF 6
          V+KEGF
Sbjct: 5  VLKEGF 10
>NP_031394
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VIKEGF 6
           V+KEGF
Sbjct: 577 VLKEGF 582
>NP_003319
          Length = 527

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IKEGFRM 8
           I EGFR+
Sbjct: 479 ISEGFRL 485
>XP_005261992
          Length = 1867

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    EGFRML 9
            EGFR+L
Sbjct: 1447 EGFRLL 1452
>XP_005261991
          Length = 1868

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    EGFRML 9
            EGFR+L
Sbjct: 1448 EGFRLL 1453
>XP_005261989
          Length = 1891

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    EGFRML 9
            EGFR+L
Sbjct: 1471 EGFRLL 1476
>XP_005261988
          Length = 1894

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    EGFRML 9
            EGFR+L
Sbjct: 1474 EGFRLL 1479
>XP_005255619
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IKEGFRML 9
           +KEGF +L
Sbjct: 292 MKEGFSLL 299
>XP_005266253
          Length = 451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VIKEGF 6
           V+KEGF
Sbjct: 194 VLKEGF 199
>XP_005268521
          Length = 1042

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IKEGFRM 8
           IK G+RM
Sbjct: 853 IKRGYRM 859
>XP_005265800
          Length = 1089

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IKEGFRM 8
           IK G+RM
Sbjct: 909 IKSGYRM 915
>XP_006714103
          Length = 1111

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IKEGFRM 8
           IK G+RM
Sbjct: 931 IKSGYRM 937
>XP_006714102
          Length = 1114

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IKEGFRM 8
           IK G+RM
Sbjct: 934 IKSGYRM 940
>NP_001099040
          Length = 375

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  EGFRML 9
          EGFR+L
Sbjct: 40 EGFRLL 45
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.340    0.157    0.435 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 574,495
Number of extensions: 930
Number of successful extensions: 67
Number of sequences better than 10000.0: 67
Number of HSP's gapped: 67
Number of HSP's successfully gapped: 67
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package