Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001155973                                                           18    1482
NP_055903                                                              18    1482
NP_001933                                                              17    4312
NP_002843                                                              17    4312
NP_689606                                                              17    4312
NP_057710                                                              17    4312
XP_005264923                                                           17    4312
NP_115622                                                              16    5631
NP_001092007                                                           16    7355
NP_689499                                                              16    7355
NP_997196                                                              16    7355
NP_003460                                                              16    7355
NP_694996                                                              16    7355
NP_067018                                                              16    7355
NP_055115                                                              16    7355
NP_005089                                                              16    7355
NP_001265227                                                           16    7355
NP_001258851                                                           16    7355
NP_001258848                                                           16    7355
NP_001258849                                                           16    7355
NP_001258847                                                           16    7355
NP_001167589                                                           16    7355
NP_976049                                                              16    7355
XP_006721755                                                           16    7355
XP_005266139                                                           16    7355
XP_005266136                                                           16    7355
XP_006717167                                                           16    7355
XP_006717166                                                           16    7355
XP_006717165                                                           16    7355
NP_001161147                                                           15    9605
NP_001161146                                                           15    9605
NP_694854                                                              15    9605
NP_001243431                                                           15    9605
NP_001243432                                                           15    9605
NP_001243433                                                           15    9605
NP_001243434                                                           15    9605
NP_001096078                                                           15    9605
NP_001273654                                                           15    9605
NP_065917                                                              15    9605
XP_005266836                                                           15    9605
XP_005247178                                                           15    9605
XP_006713145                                                           15    9605
XP_006713144                                                           15    9605
NP_150596                                                              15    9605
>NP_001155973
          Length = 1833

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VIFVVSGRE 9
           V FVVSGRE
Sbjct: 355 VNFVVSGRE 363
>NP_055903
          Length = 1723

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VIFVVSGRE 9
           V FVVSGRE
Sbjct: 355 VNFVVSGRE 363
>NP_001933
          Length = 1049

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VIFVVSGRE 9
           VIF +SG E
Sbjct: 306 VIFFISGNE 314
>NP_002843
          Length = 381

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VIFVVSGRE 9
           ++FVV G E
Sbjct: 245 IVFVVGGEE 253
>NP_689606
          Length = 621

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IFVVSGRE 9
           I+VV GRE
Sbjct: 414 IYVVGGRE 421
>NP_057710
          Length = 659

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   IFVVSGRE 9
           IFVV+GR+
Sbjct: 276 IFVVTGRK 283
>XP_005264923
          Length = 585

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IFVVSGRE 9
           I+VV GRE
Sbjct: 414 IYVVGGRE 421
>NP_115622
          Length = 569

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   IFVVSGRE 9
           +FVV+GR+
Sbjct: 110 VFVVTGRK 117
>NP_001092007
          Length = 1342

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VIFVVSG 7
           ++FVVSG
Sbjct: 521 ILFVVSG 527
>NP_689499
          Length = 1317

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VIFVVSG 7
           ++FVVSG
Sbjct: 496 ILFVVSG 502
>NP_997196
          Length = 984

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VIFVVSGRE 9
           V+F V+GR+
Sbjct: 144 VLFYVAGRD 152
>NP_003460
          Length = 617

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VIFVVSGRE 9
          +IF++SG E
Sbjct: 18 LIFLISGAE 26
>NP_694996
          Length = 2526

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    VIFVVSGRE 9
            VIFVVS  E
Sbjct: 2081 VIFVVSAGE 2089

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    VIFVVSGRE 9
            VIFV+S  E
Sbjct: 2409 VIFVISAGE 2417
>NP_067018
          Length = 220

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   VIFVVSG 7
           ++FV+SG
Sbjct: 123 IVFVISG 129
>NP_055115
          Length = 179

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  VIFVVSGRE 9
          V+F + GRE
Sbjct: 83 VVFRIEGRE 91
>NP_005089
          Length = 206

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FVVSGR 8
           FVVSGR
Sbjct: 182 FVVSGR 187
>NP_001265227
          Length = 2614

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    VIFVVSGRE 9
            VIFVVS  E
Sbjct: 2081 VIFVVSAGE 2089

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    VIFVVSGRE 9
            VIFV+S  E
Sbjct: 2409 VIFVISAGE 2417
>NP_001258851
          Length = 185

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  VIFVVSGRE 9
          V+F + GRE
Sbjct: 83 VVFRIEGRE 91
>NP_001258848
          Length = 139

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  VIFVVSGRE 9
          V+F + GRE
Sbjct: 83 VVFRIEGRE 91
>NP_001258849
          Length = 164

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  VIFVVSGRE 9
          V+F + GRE
Sbjct: 83 VVFRIEGRE 91
>NP_001258847
          Length = 153

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  VIFVVSGRE 9
          V+F + GRE
Sbjct: 57 VVFRIEGRE 65
>NP_001167589
          Length = 666

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   IFVVSGRE 9
           +F+V+GR+
Sbjct: 223 VFIVTGRK 230
>NP_976049
          Length = 651

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   IFVVSGRE 9
           +F+V+GR+
Sbjct: 223 VFIVTGRK 230
>XP_006721755
          Length = 978

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VIFVVSGRE 9
           V+F V+GR+
Sbjct: 138 VLFYVAGRD 146
>XP_005266139
          Length = 741

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VIFVVSG 7
           ++FVVSG
Sbjct: 522 ILFVVSG 528
>XP_005266136
          Length = 953

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VIFVVSG 7
           ++FVVSG
Sbjct: 522 ILFVVSG 528
>XP_006717167
          Length = 1309

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VIFVVSG 7
           ++FVVSG
Sbjct: 488 ILFVVSG 494
>XP_006717166
          Length = 1316

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VIFVVSG 7
           ++FVVSG
Sbjct: 495 ILFVVSG 501
>XP_006717165
          Length = 1343

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VIFVVSG 7
           ++FVVSG
Sbjct: 522 ILFVVSG 528
>NP_001161147
          Length = 404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VVSGRE 9
           VVSGRE
Sbjct: 284 VVSGRE 289
>NP_001161146
          Length = 435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VVSGRE 9
           VVSGRE
Sbjct: 275 VVSGRE 280
>NP_694854
          Length = 437

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VVSGRE 9
           VVSGRE
Sbjct: 277 VVSGRE 282
>NP_001243431
          Length = 327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VVSGRE 9
           VVSGRE
Sbjct: 167 VVSGRE 172
>NP_001243432
          Length = 327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VVSGRE 9
           VVSGRE
Sbjct: 167 VVSGRE 172
>NP_001243433
          Length = 287

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VVSGRE 9
           VVSGRE
Sbjct: 167 VVSGRE 172
>NP_001243434
          Length = 287

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VVSGRE 9
           VVSGRE
Sbjct: 167 VVSGRE 172
>NP_001096078
          Length = 2263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    VIFVVSGRE 9
            VIFV+S  E
Sbjct: 2084 VIFVISAGE 2092
>NP_001273654
          Length = 687

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IFVVSGRE 9
           +FV+ GR+
Sbjct: 447 LFVIGGRD 454
>NP_065917
          Length = 748

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IFVVSGRE 9
           +FV+ GR+
Sbjct: 508 LFVIGGRD 515
>XP_005266836
          Length = 161

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VIFVVSGRE 9
           V+F V GR+
Sbjct: 95  VVFKVEGRD 103
>XP_005247178
          Length = 2263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    VIFVVSGRE 9
            VIFV+S  E
Sbjct: 2084 VIFVISAGE 2092
>XP_006713145
          Length = 395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VVSGRE 9
           VVSGRE
Sbjct: 275 VVSGRE 280
>XP_006713144
          Length = 435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VVSGRE 9
           VVSGRE
Sbjct: 275 VVSGRE 280
>NP_150596
          Length = 192

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VIFVVSGRE 9
           V+F V GR+
Sbjct: 95  VVFKVEGRD 103
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.151    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 496,643
Number of extensions: 701
Number of successful extensions: 46
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 46
Number of HSP's successfully gapped: 46
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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