Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              19     665
NP_001263689                                                           19     665
NP_001119584                                                           19     665
NP_001119586                                                           19     665
NP_001263625                                                           19     665
NP_001119590                                                           19     665
NP_001263690                                                           19     665
NP_001119585                                                           19     665
NP_001263624                                                           19     665
NP_001119588                                                           19     665
NP_001119587                                                           19     665
NP_001119589                                                           19     665
NP_064581                                                              17    2528
NP_001263627                                                           16    5631
NP_001263628                                                           16    5631
NP_001263626                                                           16    5631
NP_003310                                                              16    7355
NP_597676                                                              16    7355
NP_001243779                                                           16    7355
NP_596869                                                              16    7355
NP_001193976                                                           16    7355
NP_001484                                                              16    7355
NP_647537                                                              16    7355
NP_647538                                                              16    7355
NP_001485                                                              16    7355
NP_001108628                                                           16    7355
XP_005260918                                                           16    7355
XP_005260917                                                           16    7355
XP_005246888                                                           16    7355
XP_006712788                                                           16    7355
XP_006712787                                                           16    7355
XP_006712786                                                           16    7355
NP_612455                                                              16    7355
NP_001254479                                                           16    7355
NP_597681                                                              16    7355
NP_001186681                                                           15    9605
NP_001129524                                                           15    9605
NP_689572                                                              15    9605
NP_001186682                                                           15    9605
XP_006723073                                                           15    9605
XP_006723072                                                           15    9605
XP_005258564                                                           15    9605
XP_005258563                                                           15    9605
XP_005258562                                                           15    9605
>NP_000537
          Length = 393

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VHAMAIYKQ 9
           V AMAIYKQ
Sbjct: 157 VRAMAIYKQ 165
>NP_001263689
          Length = 354

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VHAMAIYKQ 9
           V AMAIYKQ
Sbjct: 118 VRAMAIYKQ 126
>NP_001119584
          Length = 393

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VHAMAIYKQ 9
           V AMAIYKQ
Sbjct: 157 VRAMAIYKQ 165
>NP_001119586
          Length = 341

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VHAMAIYKQ 9
           V AMAIYKQ
Sbjct: 157 VRAMAIYKQ 165
>NP_001263625
          Length = 302

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VHAMAIYKQ 9
           V AMAIYKQ
Sbjct: 118 VRAMAIYKQ 126
>NP_001119590
          Length = 354

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VHAMAIYKQ 9
           V AMAIYKQ
Sbjct: 118 VRAMAIYKQ 126
>NP_001263690
          Length = 354

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VHAMAIYKQ 9
           V AMAIYKQ
Sbjct: 118 VRAMAIYKQ 126
>NP_001119585
          Length = 346

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VHAMAIYKQ 9
           V AMAIYKQ
Sbjct: 157 VRAMAIYKQ 165
>NP_001263624
          Length = 307

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VHAMAIYKQ 9
           V AMAIYKQ
Sbjct: 118 VRAMAIYKQ 126
>NP_001119588
          Length = 209

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  VHAMAIYKQ 9
          V AMAIYKQ
Sbjct: 25 VRAMAIYKQ 33
>NP_001119587
          Length = 261

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  VHAMAIYKQ 9
          V AMAIYKQ
Sbjct: 25 VRAMAIYKQ 33
>NP_001119589
          Length = 214

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  VHAMAIYKQ 9
          V AMAIYKQ
Sbjct: 25 VRAMAIYKQ 33
>NP_064581
          Length = 855

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   HAMAIYKQ 9
           HAMA+Y++
Sbjct: 611 HAMAVYER 618
>NP_001263627
          Length = 182

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4 MAIYKQ 9
         MAIYKQ
Sbjct: 1 MAIYKQ 6
>NP_001263628
          Length = 187

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4 MAIYKQ 9
         MAIYKQ
Sbjct: 1 MAIYKQ 6
>NP_001263626
          Length = 234

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4 MAIYKQ 9
         MAIYKQ
Sbjct: 1 MAIYKQ 6
>NP_003310
          Length = 26926

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2     HAMAIYKQ 9
             HA A Y+Q
Sbjct: 25685 HAQAAYRQ 25692
>NP_597676
          Length = 27051

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2     HAMAIYKQ 9
             HA A Y+Q
Sbjct: 25810 HAQAAYRQ 25817
>NP_001243779
          Length = 34350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2     HAMAIYKQ 9
             HA A Y+Q
Sbjct: 33109 HAQAAYRQ 33116
>NP_596869
          Length = 33423

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2     HAMAIYKQ 9
             HA A Y+Q
Sbjct: 32182 HAQAAYRQ 32189
>NP_001193976
          Length = 1156

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   HAMAIYK 8
           H++A+YK
Sbjct: 279 HSLALYK 285
>NP_001484
          Length = 447

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   HAMAIYK 8
           HA+A+Y+
Sbjct: 187 HALALYR 193
>NP_647537
          Length = 1429

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   HAMAIYK 8
           H++A+YK
Sbjct: 395 HSLALYK 401
>NP_647538
          Length = 1272

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   HAMAIYK 8
           H++A+YK
Sbjct: 395 HSLALYK 401
>NP_001485
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   HAMAIYK 8
           HA+A+Y+
Sbjct: 187 HALALYR 193
>NP_001108628
          Length = 400

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   HAMAIYK 8
           HA+A+Y+
Sbjct: 142 HALALYR 148
>XP_005260918
          Length = 1111

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   HAMAIYK 8
           H++A+YK
Sbjct: 395 HSLALYK 401
>XP_005260917
          Length = 1289

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   HAMAIYK 8
           H++A+YK
Sbjct: 395 HSLALYK 401
>XP_005246888
          Length = 22663

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2     HAMAIYKQ 9
             HA A Y+Q
Sbjct: 21422 HAQAAYRQ 21429
>XP_006712788
          Length = 24022

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2     HAMAIYKQ 9
             HA A Y+Q
Sbjct: 22781 HAQAAYRQ 22788
>XP_006712787
          Length = 33978

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2     HAMAIYKQ 9
             HA A Y+Q
Sbjct: 32737 HAQAAYRQ 32744
>XP_006712786
          Length = 35484

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2     HAMAIYKQ 9
             HA A Y+Q
Sbjct: 34243 HAQAAYRQ 34250
>NP_612455
          Length = 234

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VHAMAIY 7
           +HAMA Y
Sbjct: 135 LHAMAFY 141
>NP_001254479
          Length = 35991

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2     HAMAIYKQ 9
             HA A Y+Q
Sbjct: 34750 HAQAAYRQ 34757
>NP_597681
          Length = 27118

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2     HAMAIYKQ 9
             HA A Y+Q
Sbjct: 25877 HAQAAYRQ 25884
>NP_001186681
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   VHAMAIYK 8
           VHA+ +Y+
Sbjct: 276 VHALLLYR 283
>NP_001129524
          Length = 792

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   VHAMAIYK 8
           VHA+ +Y+
Sbjct: 265 VHALLLYR 272
>NP_689572
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   VHAMAIYK 8
           VHA+ +Y+
Sbjct: 276 VHALLLYR 283
>NP_001186682
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   VHAMAIYK 8
           VHA+ +Y+
Sbjct: 276 VHALLLYR 283
>XP_006723073
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   VHAMAIYK 8
           VHA+ +Y+
Sbjct: 276 VHALLLYR 283
>XP_006723072
          Length = 674

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   VHAMAIYK 8
           VHA+ +Y+
Sbjct: 114 VHALLLYR 121
>XP_005258564
          Length = 752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   VHAMAIYK 8
           VHA+ +Y+
Sbjct: 276 VHALLLYR 283
>XP_005258563
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   VHAMAIYK 8
           VHA+ +Y+
Sbjct: 276 VHALLLYR 283
>XP_005258562
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   VHAMAIYK 8
           VHA+ +Y+
Sbjct: 276 VHALLLYR 283
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.129    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 429,701
Number of extensions: 580
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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