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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              18    1482
NP_003931                                                              16    7355
NP_061986                                                              16    7355
XP_005258788                                                           16    7355
NP_110395                                                              15    9605
NP_001263215                                                           15    9605
XP_005246163                                                           15    9605
XP_006712847                                                           15    9605

>NP_443179
          Length = 2170

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VGVRSSRFS 9
           VGVRSSR S
Sbjct: 410 VGVRSSRVS 418



>NP_003931
          Length = 863

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   GVRSSRFS 9
           GV++SRF+
Sbjct: 568 GVKTSRFA 575



>NP_061986
          Length = 209

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VGVRSSRF 8
           +GV++SRF
Sbjct: 94  LGVKTSRF 101



>XP_005258788
          Length = 209

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VGVRSSRF 8
           +GV++SRF
Sbjct: 94  LGVKTSRF 101



>NP_110395
          Length = 392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   GVRSSRFS 9
           G+R+S+F+
Sbjct: 156 GIRASKFA 163



>NP_001263215
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VGVRSSR 7
           VG RSSR
Sbjct: 332 VGARSSR 338



>XP_005246163
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  GVRSSRFS 9
          G+R+S+F+
Sbjct: 36 GIRASKFA 43



>XP_006712847
          Length = 324

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  GVRSSRFS 9
          G+R+S+F+
Sbjct: 88 GIRASKFA 95



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.325    0.131    0.343 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 307,809
Number of extensions: 302
Number of successful extensions: 8
Number of sequences better than 10000.0: 8
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)