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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              18    1135
NP_001137411                                                           17    4312
NP_001137410                                                           17    4312
NP_037425                                                              16    5631
NP_004765                                                              16    7355
NP_001188301                                                           16    7355
NP_073579                                                              16    7355
XP_005257522                                                           16    7355
XP_006716154                                                           16    7355
XP_005250597                                                           16    7355
XP_005250596                                                           16    7355
XP_005250595                                                           16    7355
XP_005250594                                                           16    7355
XP_005250593                                                           16    7355
XP_006716152                                                           16    7355
XP_005250592                                                           16    7355
XP_005250591                                                           16    7355
NP_001254645                                                           15    9605
NP_001257293                                                           15    9605
NP_003704                                                              15    9605
NP_036322                                                              15    9605
NP_001257294                                                           15    9605
NP_689502                                                              15    9605
XP_006723002                                                           15    9605
XP_005260208                                                           15    9605
XP_005260207                                                           15    9605
XP_006723001                                                           15    9605
XP_006722813                                                           15    9605
XP_006713545                                                           15    9605
XP_006713544                                                           15    9605
NP_114171                                                              15    9605

>NP_443179
          Length = 2170

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VGSSRVSKH 9
           V SSRVSKH
Sbjct: 412 VRSSRVSKH 420



>NP_001137411
          Length = 674

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   VGSSRVSKH 9
           +G+SR+++H
Sbjct: 405 IGNSRLARH 413



>NP_001137410
          Length = 661

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   VGSSRVSKH 9
           +G+SR+++H
Sbjct: 392 IGNSRLARH 400



>NP_037425
          Length = 282

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SRVSKH 9
          SRVSKH
Sbjct: 40 SRVSKH 45



>NP_004765
          Length = 1581

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    GSSRVSKH 9
            GSS ++KH
Sbjct: 1154 GSSPITKH 1161



>NP_001188301
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VGSSRVSKH 9
          VGS  V+KH
Sbjct: 90 VGSLSVNKH 98



>NP_073579
          Length = 1154

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VGSSRVSKH 9
          VGS  V+KH
Sbjct: 40 VGSLSVNKH 48



>XP_005257522
          Length = 1409

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GSSRVSKH 9
           GSS ++KH
Sbjct: 982 GSSPITKH 989



>XP_006716154
          Length = 446

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VGSSRVSKH 9
          VGS  V+KH
Sbjct: 90 VGSLSVNKH 98



>XP_005250597
          Length = 602

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VGSSRVSKH 9
          VGS  V+KH
Sbjct: 90 VGSLSVNKH 98



>XP_005250596
          Length = 618

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VGSSRVSKH 9
          VGS  V+KH
Sbjct: 90 VGSLSVNKH 98



>XP_005250595
          Length = 1007

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VGSSRVSKH 9
          VGS  V+KH
Sbjct: 90 VGSLSVNKH 98



>XP_005250594
          Length = 1142

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VGSSRVSKH 9
          VGS  V+KH
Sbjct: 90 VGSLSVNKH 98



>XP_005250593
          Length = 1195

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VGSSRVSKH 9
          VGS  V+KH
Sbjct: 90 VGSLSVNKH 98



>XP_006716152
          Length = 1198

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VGSSRVSKH 9
          VGS  V+KH
Sbjct: 90 VGSLSVNKH 98



>XP_005250592
          Length = 1207

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VGSSRVSKH 9
          VGS  V+KH
Sbjct: 90 VGSLSVNKH 98



>XP_005250591
          Length = 1207

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VGSSRVSKH 9
          VGS  V+KH
Sbjct: 90 VGSLSVNKH 98



>NP_001254645
          Length = 622

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VGSSRVSKH 9
           + SSR+S+H
Sbjct: 546 IWSSRLSEH 554



>NP_001257293
          Length = 912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GSSRVSKH 9
           GS+ V KH
Sbjct: 660 GSTEVGKH 667



>NP_003704
          Length = 311

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GSSRVSKH 9
           G SRVS H
Sbjct: 242 GLSRVSDH 249



>NP_036322
          Length = 902

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GSSRVSKH 9
           GS+ V KH
Sbjct: 650 GSTEVGKH 657



>NP_001257294
          Length = 801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GSSRVSKH 9
           GS+ V KH
Sbjct: 549 GSTEVGKH 556



>NP_689502
          Length = 486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VGSSRVSKH 9
           + SSR SKH
Sbjct: 403 ITSSRRSKH 411




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SSRVSKH 9
           SSR+S+H
Sbjct: 461 SSRLSRH 467



>XP_006723002
          Length = 265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VGSSRVSKH 9
           + SSR SKH
Sbjct: 182 ITSSRRSKH 190




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SSRVSKH 9
           SSR+S+H
Sbjct: 240 SSRLSRH 246



>XP_005260208
          Length = 379

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VGSSRVSKH 9
           + SSR SKH
Sbjct: 296 ITSSRRSKH 304




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SSRVSKH 9
           SSR+S+H
Sbjct: 354 SSRLSRH 360



>XP_005260207
          Length = 486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VGSSRVSKH 9
           + SSR SKH
Sbjct: 403 ITSSRRSKH 411




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SSRVSKH 9
           SSR+S+H
Sbjct: 461 SSRLSRH 467



>XP_006723001
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VGSSRVSKH 9
           + SSR SKH
Sbjct: 409 ITSSRRSKH 417




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SSRVSKH 9
           SSR+S+H
Sbjct: 467 SSRLSRH 473



>XP_006722813
          Length = 594

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VGSSRVSKH 9
           + SSR+S+H
Sbjct: 518 IWSSRLSEH 526



>XP_006713545
          Length = 900

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GSSRVSKH 9
           GS+ V KH
Sbjct: 648 GSTEVGKH 655



>XP_006713544
          Length = 902

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GSSRVSKH 9
           GS+ V KH
Sbjct: 650 GSTEVGKH 657



>NP_114171
          Length = 798

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SSRVSKH 9
           SSR SKH
Sbjct: 336 SSRKSKH 342



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.306    0.116    0.303 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 405,557
Number of extensions: 728
Number of successful extensions: 36
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)