Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              19     869
XP_005266703                                                           19     869
NP_000246                                                              17    3301
XP_005249200                                                           17    3301
XP_005273894                                                           17    4312
NP_878253                                                              17    4312
NP_001278017                                                           16    7355
NP_114157                                                              16    7355
XP_006723474                                                           16    7355
XP_005259362                                                           16    7355
NP_064446                                                              16    7355
NP_064425                                                              15    9605
NP_001001290                                                           15    9605
NP_037528                                                              15    9605
NP_001269970                                                           15    9605
NP_001269971                                                           15    9605
NP_001002021                                                           15    9605
NP_002617                                                              15    9605
NP_001160158                                                           15    9605
NP_001164562                                                           15    9605
NP_079517                                                              15    9605
NP_060538                                                              15    9605
NP_001129193                                                           15    9605
NP_775885                                                              15    9605
NP_001004060                                                           15    9605
NP_848620                                                              15    9605
NP_001004067                                                           15    9605
NP_000280                                                              15    9605
NP_001160160                                                           15    9605
XP_006725258                                                           15    9605
XP_005261194                                                           15    9605
XP_005261193                                                           15    9605
XP_005261192                                                           15    9605
XP_006724075                                                           15    9605
XP_006724074                                                           15    9605
XP_005261006                                                           15    9605
XP_006724055                                                           15    9605
XP_005255375                                                           15    9605
XP_006719527                                                           15    9605
XP_006719526                                                           15    9605
XP_005269037                                                           15    9605
XP_005269036                                                           15    9605
XP_005269035                                                           15    9605
XP_005269034                                                           15    9605
XP_005269033                                                           15    9605
XP_005269032                                                           15    9605
XP_005269031                                                           15    9605
XP_005273608                                                           15    9605
XP_005273607                                                           15    9605
XP_006714032                                                           15    9605
XP_006714031                                                           15    9605
NP_001160159                                                           15    9605
NP_055102                                                              15    9605
>NP_443179
          Length = 2170

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VGIETLAKG 9
            VGI TLAKG
Sbjct: 2091 VGIATLAKG 2099
>XP_005266703
          Length = 1501

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VGIETLAKG 9
            VGI TLAKG
Sbjct: 1422 VGIATLAKG 1430
>NP_000246
          Length = 750

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   VGIETLAKG 9
           VGI TLA G
Sbjct: 669 VGISTLAAG 677
>XP_005249200
          Length = 750

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   VGIETLAKG 9
           VGI TLA G
Sbjct: 669 VGISTLAAG 677
>XP_005273894
          Length = 520

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VGIETLAK 8
           V IETLAK
Sbjct: 388 VSIETLAK 395
>NP_878253
          Length = 520

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VGIETLAK 8
           V IETLAK
Sbjct: 388 VSIETLAK 395
>NP_001278017
          Length = 2912

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    GIETLAKG 9
            G ETL+KG
Sbjct: 2019 GKETLSKG 2026
>NP_114157
          Length = 2873

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    GIETLAKG 9
            G ETL+KG
Sbjct: 1980 GKETLSKG 1987
>XP_006723474
          Length = 2737

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    GIETLAKG 9
            G ETL+KG
Sbjct: 1844 GKETLSKG 1851
>XP_005259362
          Length = 2855

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    GIETLAKG 9
            G ETL+KG
Sbjct: 1962 GKETLSKG 1969
>NP_064446
          Length = 387

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VGIETLAKG 9
           VG++TL  G
Sbjct: 248 VGVDTLTDG 256
>NP_064425
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GIETLA 7
           GIETLA
Sbjct: 362 GIETLA 367
>NP_001001290
          Length = 511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GIETLA 7
           GIETLA
Sbjct: 333 GIETLA 338
>NP_037528
          Length = 1055

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   VGIETL 6
           VGIETL
Sbjct: 676 VGIETL 681
>NP_001269970
          Length = 1125

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   VGIETL 6
           VGIETL
Sbjct: 676 VGIETL 681
>NP_001269971
          Length = 1087

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   VGIETL 6
           VGIETL
Sbjct: 676 VGIETL 681
>NP_001002021
          Length = 830

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 488 GFEGLAKG 495
>NP_002617
          Length = 780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 438 GFEGLAKG 445
>NP_001160158
          Length = 851

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 510 GFEGLAKG 517
>NP_001164562
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   VGIETLAK 8
           +G++T+AK
Sbjct: 120 LGVDTIAK 127
>NP_079517
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   VGIETLAK 8
           +G++T+AK
Sbjct: 173 LGVDTIAK 180
>NP_060538
          Length = 973

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  VGIETLAK 8
          +GIET++K
Sbjct: 78 LGIETVSK 85
>NP_001129193
          Length = 973

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  VGIETLAK 8
          +GIET++K
Sbjct: 78 LGIETVSK 85
>NP_775885
          Length = 1222

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VGIETLAKG 9
           VGI  L+KG
Sbjct: 614 VGIYNLSKG 622
>NP_001004060
          Length = 1267

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VGIETLAKG 9
           VGI  L+KG
Sbjct: 614 VGIYNLSKG 622
>NP_848620
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VGIETLAK 8
           VG+ T+AK
Sbjct: 313 VGLSTMAK 320
>NP_001004067
          Length = 1222

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VGIETLAKG 9
           VGI  L+KG
Sbjct: 614 VGIYNLSKG 622
>NP_000280
          Length = 780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 439 GFEGLAKG 446
>NP_001160160
          Length = 780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 439 GFEGLAKG 446
>XP_006725258
          Length = 1267

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VGIETLAKG 9
           VGI  L+KG
Sbjct: 614 VGIYNLSKG 622
>XP_005261194
          Length = 751

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 409 GFEGLAKG 416
>XP_005261193
          Length = 774

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 432 GFEGLAKG 439
>XP_005261192
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 461 GFEGLAKG 468
>XP_006724075
          Length = 830

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 488 GFEGLAKG 495
>XP_006724074
          Length = 853

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 511 GFEGLAKG 518
>XP_005261006
          Length = 721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   VGIETL 6
           VGIETL
Sbjct: 272 VGIETL 277
>XP_006724055
          Length = 826

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   VGIETL 6
           VGIETL
Sbjct: 377 VGIETL 382
>XP_005255375
          Length = 1267

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VGIETLAKG 9
           VGI  L+KG
Sbjct: 614 VGIYNLSKG 622
>XP_006719527
          Length = 780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 439 GFEGLAKG 446
>XP_006719526
          Length = 780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 439 GFEGLAKG 446
>XP_005269037
          Length = 811

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 470 GFEGLAKG 477
>XP_005269036
          Length = 851

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 510 GFEGLAKG 517
>XP_005269035
          Length = 851

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 510 GFEGLAKG 517
>XP_005269034
          Length = 851

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 510 GFEGLAKG 517
>XP_005269033
          Length = 883

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 542 GFEGLAKG 549
>XP_005269032
          Length = 883

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 542 GFEGLAKG 549
>XP_005269031
          Length = 883

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 542 GFEGLAKG 549
>XP_005273608
          Length = 804

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  VGIETLAK 8
          +GIET++K
Sbjct: 78 LGIETVSK 85
>XP_005273607
          Length = 937

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  VGIETLAK 8
          +GIET++K
Sbjct: 78 LGIETVSK 85
>XP_006714032
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GIETLA 7
           GIETLA
Sbjct: 333 GIETLA 338
>XP_006714031
          Length = 563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GIETLA 7
           GIETLA
Sbjct: 362 GIETLA 367
>NP_001160159
          Length = 780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GIETLAKG 9
           G E LAKG
Sbjct: 439 GFEGLAKG 446
>NP_055102
          Length = 1222

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VGIETLAKG 9
           VGI  L+KG
Sbjct: 614 VGIYNLSKG 622
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.138    0.361 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 389,450
Number of extensions: 641
Number of successful extensions: 53
Number of sequences better than 10000.0: 53
Number of HSP's gapped: 53
Number of HSP's successfully gapped: 53
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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