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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001129047                                                           17    3301
NP_536724                                                              17    3301
NP_000360                                                              17    3301
XP_005268094                                                           17    3301
NP_071319                                                              17    4312
NP_061123                                                              17    4312
XP_005257571                                                           17    4312
XP_005257570                                                           17    4312
XP_005257569                                                           17    4312
NP_061722                                                              16    5631
NP_001248770                                                           16    5631
XP_005255642                                                           16    5631
NP_001098718                                                           16    5631
NP_079056                                                              16    5631
NP_114089                                                              16    5631
NP_001005487                                                           16    5631
NP_115655                                                              16    7355
XP_005247886                                                           16    7355
XP_006713849                                                           16    7355
NP_001026851                                                           15    9605
NP_004928                                                              15    9605
NP_071351                                                              15    9605
NP_001231875                                                           15    9605
NP_061111                                                              15    9605
NP_064732                                                              15    9605
NP_001230723                                                           15    9605
NP_004293                                                              15    9605
NP_064733                                                              15    9605
NP_001230724                                                           15    9605
XP_006721527                                                           15    9605
XP_006721526                                                           15    9605
XP_005256542                                                           15    9605
XP_006719748                                                           15    9605

>NP_001129047
          Length = 91

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  VFVLTLIIV 9
          VFV TL+IV
Sbjct: 11 VFVATLLIV 19



>NP_536724
          Length = 435

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VFVLTLIIV 9
           +FVLT II+
Sbjct: 355 IFVLTCIII 363



>NP_000360
          Length = 764

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VFVLTLIIV 9
           VFVLTL IV
Sbjct: 584 VFVLTLNIV 592



>XP_005268094
          Length = 764

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VFVLTLIIV 9
           VFVLTL IV
Sbjct: 584 VFVLTLNIV 592



>NP_071319
          Length = 486

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   FVLTLIIV 9
           FVLT+I+V
Sbjct: 110 FVLTIILV 117



>NP_061123
          Length = 453

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  FVLTLIIV 9
          FVLT+I+V
Sbjct: 77 FVLTIILV 84



>XP_005257571
          Length = 496

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   FVLTLIIV 9
           FVLT+I+V
Sbjct: 110 FVLTIILV 117



>XP_005257570
          Length = 542

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   FVLTLIIV 9
           FVLT+I+V
Sbjct: 165 FVLTIILV 172



>XP_005257569
          Length = 551

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   FVLTLIIV 9
           FVLT+I+V
Sbjct: 165 FVLTIILV 172



>NP_061722
          Length = 1007

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   VFVLTLIIV 9
           +F+LT+II+
Sbjct: 718 IFLLTIIIL 726



>NP_001248770
          Length = 1006

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   FVLTLIIV 9
           F+LTLI++
Sbjct: 909 FILTLIVL 916



>XP_005255642
          Length = 647

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   FVLTLIIV 9
           F+LTLI++
Sbjct: 550 FILTLIVL 557



>NP_001098718
          Length = 1006

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   FVLTLIIV 9
           F+LTLI++
Sbjct: 909 FILTLIVL 916



>NP_079056
          Length = 760

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   FVLTLIIV 9
           F+LTLI++
Sbjct: 663 FILTLIVL 670



>NP_114089
          Length = 884

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   VFVLTLIIV 9
           +F+LT+II+
Sbjct: 718 IFLLTIIIL 726



>NP_001005487
          Length = 307

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VFVLTLIIV 9
          VF+LTL +V
Sbjct: 58 VFLLTLAVV 66



>NP_115655
          Length = 1196

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VFVLTLII 8
            +FVL LII
Sbjct: 1071 IFVLVLII 1078



>XP_005247886
          Length = 1127

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VFVLTLII 8
            +FVL LII
Sbjct: 1071 IFVLVLII 1078



>XP_006713849
          Length = 1147

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VFVLTLII 8
            +FVL LII
Sbjct: 1022 IFVLVLII 1029



>NP_001026851
          Length = 400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VLTLIIV 9
           VLTLII+
Sbjct: 214 VLTLIII 220



>NP_004928
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VLTLIIV 9
           VLTLII+
Sbjct: 214 VLTLIII 220



>NP_071351
          Length = 2752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    FVLTLIIV 9
            FVLT+I +
Sbjct: 1300 FVLTIIFI 1307



>NP_001231875
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VFVLTLII 8
           VF+LTL++
Sbjct: 412 VFLLTLLL 419



>NP_061111
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VFVLTLIIV 9
          VF++ LIIV
Sbjct: 16 VFMILLIIV 24



>NP_064732
          Length = 453

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VFVLTLIIV 9
          VF+L LII+
Sbjct: 83 VFLLFLIII 91



>NP_001230723
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VFVLTLIIV 9
          VF++ LIIV
Sbjct: 16 VFMILLIIV 24



>NP_004293
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VFVLTLIIV 9
           VF+L LII+
Sbjct: 135 VFLLFLIII 143



>NP_064733
          Length = 546

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VFVLTLIIV 9
           VF+L LII+
Sbjct: 135 VFLLFLIII 143



>NP_001230724
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VFVLTLIIV 9
          VF++ LIIV
Sbjct: 16 VFMILLIIV 24



>XP_006721527
          Length = 253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  VLTLIIV 9
          VLTLII+
Sbjct: 67 VLTLIII 73



>XP_006721526
          Length = 400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VLTLIIV 9
           VLTLII+
Sbjct: 214 VLTLIII 220



>XP_005256542
          Length = 400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VLTLIIV 9
           VLTLII+
Sbjct: 214 VLTLIII 220



>XP_006719748
          Length = 545

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VFVLTLIIV 9
           VF+L LII+
Sbjct: 135 VFLLFLIII 143



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.359    0.174    0.439 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 292,478
Number of extensions: 447
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.0 bits)
S2: 28 (15.4 bits)