Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     509
XP_005262424                                                           20     509
NP_149016                                                              17    3301
XP_005265460                                                           17    3301
NP_775764                                                              17    4312
NP_001032208                                                           16    5631
NP_001231817                                                           16    5631
NP_055729                                                              16    5631
XP_006712304                                                           16    5631
XP_006712303                                                           16    5631
XP_006712302                                                           16    5631
XP_006712301                                                           16    5631
XP_006712300                                                           16    5631
XP_005246116                                                           16    5631
XP_006712299                                                           16    5631
XP_005246115                                                           16    5631
XP_006712298                                                           16    5631
XP_006712297                                                           16    5631
NP_056197                                                              16    7355
NP_001268229                                                           16    7355
NP_963848                                                              16    7355
NP_001336                                                              16    7355
NP_958852                                                              16    7355
NP_114152                                                              16    7355
XP_005267560                                                           16    7355
XP_005267559                                                           16    7355
XP_006719323                                                           16    7355
XP_005268745                                                           16    7355
XP_006715912                                                           16    7355
NP_958853                                                              16    7355
NP_000050                                                              15    9605
NP_001271282                                                           15    9605
NP_001271281                                                           15    9605
NP_065826                                                              15    9605
NP_001253977                                                           15    9605
NP_055045                                                              15    9605
NP_066363                                                              15    9605
XP_006723899                                                           15    9605
NP_005399                                                              15    9605
>NP_722576
          Length = 3080

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    FTSKKVSD 9
            FTSKKVSD
Sbjct: 1523 FTSKKVSD 1530
>XP_005262424
          Length = 2875

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    FTSKKVSD 9
            FTSKKVSD
Sbjct: 1318 FTSKKVSD 1325
>NP_149016
          Length = 589

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   FTSKKVSD 9
           +TS+KVSD
Sbjct: 520 YTSQKVSD 527
>XP_005265460
          Length = 589

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   FTSKKVSD 9
           +TS+KVSD
Sbjct: 520 YTSQKVSD 527
>NP_775764
          Length = 773

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VFTSKKVSD 9
           ++TSK VSD
Sbjct: 184 LYTSKAVSD 192
>NP_001032208
          Length = 857

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VFTSKKVSD 9
           +FTS+K SD
Sbjct: 317 LFTSRKGSD 325
>NP_001231817
          Length = 405

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VFTSKKVSD 9
           +FTS+K SD
Sbjct: 317 LFTSRKGSD 325
>NP_055729
          Length = 804

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VFTSKKVSD 9
           +FTS+K SD
Sbjct: 317 LFTSRKGSD 325
>XP_006712304
          Length = 460

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VFTSKKVSD 9
           +FTS+K SD
Sbjct: 321 LFTSRKGSD 329
>XP_006712303
          Length = 465

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VFTSKKVSD 9
           +FTS+K SD
Sbjct: 321 LFTSRKGSD 329
>XP_006712302
          Length = 782

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VFTSKKVSD 9
           +FTS+K SD
Sbjct: 217 LFTSRKGSD 225
>XP_006712301
          Length = 748

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VFTSKKVSD 9
           +FTS+K SD
Sbjct: 183 LFTSRKGSD 191
>XP_006712300
          Length = 748

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VFTSKKVSD 9
           +FTS+K SD
Sbjct: 183 LFTSRKGSD 191
>XP_005246116
          Length = 808

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VFTSKKVSD 9
           +FTS+K SD
Sbjct: 321 LFTSRKGSD 329
>XP_006712299
          Length = 833

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VFTSKKVSD 9
           +FTS+K SD
Sbjct: 321 LFTSRKGSD 329
>XP_005246115
          Length = 861

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VFTSKKVSD 9
           +FTS+K SD
Sbjct: 321 LFTSRKGSD 329
>XP_006712298
          Length = 882

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VFTSKKVSD 9
           +FTS+K SD
Sbjct: 317 LFTSRKGSD 325
>XP_006712297
          Length = 886

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VFTSKKVSD 9
           +FTS+K SD
Sbjct: 321 LFTSRKGSD 329
>NP_056197
          Length = 2610

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    FTSKKVS 8
            FTSKK++
Sbjct: 2036 FTSKKIT 2042
>NP_001268229
          Length = 580

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VFTSKKVSD 9
           +FTS+K +D
Sbjct: 146 IFTSRKGAD 154
>NP_963848
          Length = 735

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  FTSKKVSD 9
          +++KKVSD
Sbjct: 22 YSTKKVSD 29
>NP_001336
          Length = 735

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  FTSKKVSD 9
          +++KKVSD
Sbjct: 22 YSTKKVSD 29
>NP_958852
          Length = 735

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  FTSKKVSD 9
          +++KKVSD
Sbjct: 22 YSTKKVSD 29
>NP_114152
          Length = 911

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VFTSKKVSD 9
           +FTS+K +D
Sbjct: 374 IFTSRKGAD 382
>XP_005267560
          Length = 2563

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    FTSKKVS 8
            FTSKK++
Sbjct: 1989 FTSKKIT 1995
>XP_005267559
          Length = 2610

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    FTSKKVS 8
            FTSKK++
Sbjct: 2036 FTSKKIT 2042
>XP_006719323
          Length = 455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  FTSKKVSD 9
          +++KKVSD
Sbjct: 60 YSTKKVSD 67
>XP_005268745
          Length = 773

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  FTSKKVSD 9
          +++KKVSD
Sbjct: 60 YSTKKVSD 67
>XP_006715912
          Length = 679

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VFTSKKVSD 9
           +FTS+K +D
Sbjct: 142 IFTSRKGAD 150
>NP_958853
          Length = 735

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  FTSKKVSD 9
          +++KKVSD
Sbjct: 22 YSTKKVSD 29
>NP_000050
          Length = 3418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SKKVSD 9
           SKKVSD
Sbjct: 723 SKKVSD 728
>NP_001271282
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VFTSKK 6
           +FTSKK
Sbjct: 636 IFTSKK 641
>NP_001271281
          Length = 902

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VFTSKK 6
           +FTSKK
Sbjct: 872 IFTSKK 877
>NP_065826
          Length = 1013

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VFTSKK 6
           +FTSKK
Sbjct: 974 IFTSKK 979
>NP_001253977
          Length = 926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VFTSKK 6
           +FTSKK
Sbjct: 887 IFTSKK 892
>NP_055045
          Length = 627

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FTSKKVSD 9
           FT KKV++
Sbjct: 108 FTGKKVTE 115
>NP_066363
          Length = 1918

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    FTSKKVSD 9
            FTS+++SD
Sbjct: 1748 FTSQELSD 1755
>XP_006723899
          Length = 1918

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    FTSKKVSD 9
            FTS+++SD
Sbjct: 1748 FTSQELSD 1755
>NP_005399
          Length = 98

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  FTSKKVS 8
          F+SKK+S
Sbjct: 38 FSSKKIS 44
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.120    0.295 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 373,682
Number of extensions: 484
Number of successful extensions: 39
Number of sequences better than 10000.0: 39
Number of HSP's gapped: 39
Number of HSP's successfully gapped: 39
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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