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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           18    1135
NP_000242                                                              18    1135
XP_005264389                                                           18    1135
XP_005250956                                                           17    3301
NP_000828                                                              17    3301
NP_001009184                                                           17    3301
NP_620417                                                              17    4312
NP_001171582                                                           17    4312
NP_001171583                                                           17    4312
XP_006720485                                                           17    4312
XP_006720484                                                           17    4312
XP_006720483                                                           17    4312
NP_653251                                                              17    4312
NP_851322                                                              16    7355
NP_001265557                                                           16    7355
NP_001265555                                                           16    7355
NP_001265554                                                           16    7355
NP_001265556                                                           16    7355
NP_057736                                                              16    7355
NP_001028755                                                           15    9605
NP_055793                                                              15    9605
NP_001032248                                                           15    9605
NP_001138585                                                           15    9605
NP_001009921                                                           15    9605
XP_005274529                                                           15    9605
XP_005274528                                                           15    9605
XP_005274527                                                           15    9605
XP_006713619                                                           15    9605
XP_005247310                                                           15    9605
XP_005247308                                                           15    9605
XP_005247166                                                           15    9605
NP_056118                                                              15    9605
NP_001073909                                                           15    9605

>NP_001245210
          Length = 868

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VFTFMAEML 9
           V TFMAEML
Sbjct: 656 VSTFMAEML 664



>NP_000242
          Length = 934

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VFTFMAEML 9
           V TFMAEML
Sbjct: 722 VSTFMAEML 730



>XP_005264389
          Length = 921

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VFTFMAEML 9
           V TFMAEML
Sbjct: 722 VSTFMAEML 730



>XP_005250956
          Length = 371

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VFTFMAEM 8
           VFTF+AE+
Sbjct: 182 VFTFVAEV 189



>NP_000828
          Length = 371

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VFTFMAEM 8
           VFTF+AE+
Sbjct: 182 VFTFVAEV 189



>NP_001009184
          Length = 371

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VFTFMAEM 8
           VFTF+AE+
Sbjct: 182 VFTFVAEV 189



>NP_620417
          Length = 235

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   FTFMAEML 9
           FTFMA+++
Sbjct: 110 FTFMAQLV 117



>NP_001171582
          Length = 235

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   FTFMAEML 9
           FTFMA+++
Sbjct: 110 FTFMAQLV 117



>NP_001171583
          Length = 235

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   FTFMAEML 9
           FTFMA+++
Sbjct: 110 FTFMAQLV 117



>XP_006720485
          Length = 164

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  FTFMAEML 9
          FTFMA+++
Sbjct: 39 FTFMAQLV 46



>XP_006720484
          Length = 235

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   FTFMAEML 9
           FTFMA+++
Sbjct: 110 FTFMAQLV 117



>XP_006720483
          Length = 253

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   FTFMAEML 9
           FTFMA+++
Sbjct: 128 FTFMAQLV 135



>NP_653251
          Length = 467

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VFTFMAEM 8
           VFTF A+M
Sbjct: 368 VFTFTAQM 375



>NP_851322
          Length = 724

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VFTFMA 6
           +FTFMA
Sbjct: 589 IFTFMA 594



>NP_001265557
          Length = 687

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FTFMAEML 9
           +T+M EML
Sbjct: 155 YTYMEEML 162



>NP_001265555
          Length = 564

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  FTFMAEML 9
          +T+M EML
Sbjct: 32 YTYMEEML 39



>NP_001265554
          Length = 836

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FTFMAEML 9
           +T+M EML
Sbjct: 304 YTYMEEML 311



>NP_001265556
          Length = 564

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  FTFMAEML 9
          +T+M EML
Sbjct: 32 YTYMEEML 39



>NP_057736
          Length = 848

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FTFMAEML 9
           +T+M EML
Sbjct: 316 YTYMEEML 323



>NP_001028755
          Length = 439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   FTFMAE 7
           FTFM+E
Sbjct: 393 FTFMSE 398



>NP_055793
          Length = 1222

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   FTFMAE 7
           FTF+AE
Sbjct: 967 FTFLAE 972



>NP_001032248
          Length = 448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   FTFMAE 7
           FTFM+E
Sbjct: 402 FTFMSE 407



>NP_001138585
          Length = 307

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VFTFMAEML 9
           V+TF+A +L
Sbjct: 233 VYTFLAVLL 241



>NP_001009921
          Length = 1428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VFTFMAEML 9
           +FTF+A  L
Sbjct: 834 LFTFLARQL 842



>XP_005274529
          Length = 379

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   FTFMAE 7
           FTFM+E
Sbjct: 333 FTFMSE 338



>XP_005274528
          Length = 379

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   FTFMAE 7
           FTFM+E
Sbjct: 333 FTFMSE 338



>XP_005274527
          Length = 451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   FTFMAE 7
           FTFM+E
Sbjct: 405 FTFMSE 410



>XP_006713619
          Length = 856

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VFTFMAEML 9
           +FTF+A  L
Sbjct: 262 LFTFLARQL 270



>XP_005247310
          Length = 1035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VFTFMAEML 9
           +FTF+A  L
Sbjct: 834 LFTFLARQL 842



>XP_005247308
          Length = 1428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VFTFMAEML 9
           +FTF+A  L
Sbjct: 834 LFTFLARQL 842



>XP_005247166
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VFTFMAEM 8
          +FT MAEM
Sbjct: 58 LFTEMAEM 65



>NP_056118
          Length = 1426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VFTFMAEML 9
           +FTF+A  L
Sbjct: 832 LFTFLARQL 840



>NP_001073909
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VFTFMAEM 8
          +FT MAEM
Sbjct: 58 LFTEMAEM 65



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.353    0.147    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 354,482
Number of extensions: 460
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.0 bits)
S2: 28 (15.4 bits)