Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              18    1935
NP_671714                                                              18    1935
NP_001230647                                                           18    1935
NP_115520                                                              18    1935
NP_002860                                                              18    1935
NP_942599                                                              18    1935
NP_057661                                                              18    1935
XP_006715890                                                           18    1935
XP_005250160                                                           18    1935
XP_005250159                                                           18    1935
NP_001071105                                                           18    1935
NP_056343                                                              17    3301
XP_005256660                                                           17    3301
XP_005256659                                                           17    3301
XP_005256658                                                           17    3301
XP_005256656                                                           17    3301
XP_006721565                                                           17    3301
XP_006721564                                                           17    3301
XP_006721563                                                           17    3301
XP_005256655                                                           17    3301
XP_005256654                                                           17    3301
XP_006721562                                                           17    3301
NP_068834                                                              17    4312
XP_005250186                                                           17    4312
NP_919299                                                              16    5631
NP_659502                                                              16    5631
NP_001230064                                                           16    5631
NP_001075949                                                           16    5631
NP_001248772                                                           16    5631
NP_001230065                                                           16    5631
XP_006725158                                                           16    5631
XP_006719831                                                           16    5631
XP_006718547                                                           16    5631
XP_006716771                                                           16    5631
NP_001091980                                                           16    5631
NP_055604                                                              16    5631
NP_008835                                                              16    7355
NP_001075109                                                           16    7355
NP_002542                                                              16    7355
NP_001138502                                                           16    7355
NP_060371                                                              16    7355
XP_005260860                                                           16    7355
XP_005260859                                                           16    7355
XP_005260858                                                           16    7355
XP_005260857                                                           16    7355
XP_006723671                                                           16    7355
XP_006723670                                                           16    7355
XP_005260854                                                           16    7355
XP_006723669                                                           16    7355
XP_005253963                                                           16    7355
XP_005253962                                                           16    7355
XP_006719124                                                           16    7355
XP_005253405                                                           16    7355
NP_001137291                                                           16    7355
NP_071927                                                              16    7355
NP_777601                                                              16    7355
NP_055519                                                              15    9605
NP_689845                                                              15    9605
NP_001178249                                                           15    9605
NP_001265560                                                           15    9605
NP_055238                                                              15    9605
NP_001178250                                                           15    9605
NP_071423                                                              15    9605
NP_068712                                                              15    9605
NP_000805                                                              15    9605
XP_006718329                                                           15    9605
XP_006718328                                                           15    9605
XP_006714730                                                           15    9605
XP_005272100                                                           15    9605
XP_005248564                                                           15    9605
XP_006714650                                                           15    9605
XP_005272020                                                           15    9605
XP_005244872                                                           15    9605
NP_005900                                                              15    9605
>NP_005742
          Length = 3907

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VFLGERSVL 9
            VFL ERSVL
Sbjct: 3033 VFLEERSVL 3041
>NP_671714
          Length = 3899

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VFLGERSVL 9
            VFL ERSVL
Sbjct: 3025 VFLEERSVL 3033
>NP_001230647
          Length = 104

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1  VFLGERSV 8
          VFLGE+SV
Sbjct: 17 VFLGEQSV 24
>NP_115520
          Length = 254

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1  VFLGERSV 8
          VFLGE+SV
Sbjct: 17 VFLGEQSV 24
>NP_002860
          Length = 208

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1  VFLGERSV 8
          VFLGE+SV
Sbjct: 17 VFLGEQSV 24
>NP_942599
          Length = 208

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1  VFLGERSV 8
          VFLGE+SV
Sbjct: 17 VFLGEQSV 24
>NP_057661
          Length = 208

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1  VFLGERSV 8
          VFLGE+SV
Sbjct: 17 VFLGEQSV 24
>XP_006715890
          Length = 3860

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VFLGERSVL 9
            VFL ERSVL
Sbjct: 2986 VFLEERSVL 2994
>XP_005250160
          Length = 3939

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VFLGERSVL 9
            VFL ERSVL
Sbjct: 3065 VFLEERSVL 3073
>XP_005250159
          Length = 3952

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VFLGERSVL 9
            VFL ERSVL
Sbjct: 3078 VFLEERSVL 3086
>NP_001071105
          Length = 254

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1  VFLGERSV 8
          VFLGE+SV
Sbjct: 17 VFLGEQSV 24
>NP_056343
          Length = 350

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VFLGERS 7
           VF+GERS
Sbjct: 138 VFIGERS 144
>XP_005256660
          Length = 315

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VFLGERS 7
           VF+GERS
Sbjct: 103 VFIGERS 109
>XP_005256659
          Length = 339

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VFLGERS 7
           VF+GERS
Sbjct: 103 VFIGERS 109
>XP_005256658
          Length = 339

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VFLGERS 7
           VF+GERS
Sbjct: 103 VFIGERS 109
>XP_005256656
          Length = 350

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VFLGERS 7
           VF+GERS
Sbjct: 138 VFIGERS 144
>XP_006721565
          Length = 374

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VFLGERS 7
           VF+GERS
Sbjct: 138 VFIGERS 144
>XP_006721564
          Length = 374

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VFLGERS 7
           VF+GERS
Sbjct: 138 VFIGERS 144
>XP_006721563
          Length = 374

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VFLGERS 7
           VF+GERS
Sbjct: 138 VFIGERS 144
>XP_005256655
          Length = 374

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VFLGERS 7
           VF+GERS
Sbjct: 138 VFIGERS 144
>XP_005256654
          Length = 374

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VFLGERS 7
           VF+GERS
Sbjct: 138 VFIGERS 144
>XP_006721562
          Length = 374

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VFLGERS 7
           VF+GERS
Sbjct: 138 VFIGERS 144
>NP_068834
          Length = 450

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FLGERSVL 9
           FLG RSV+
Sbjct: 182 FLGNRSVI 189
>XP_005250186
          Length = 332

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  FLGERSVL 9
          FLG RSV+
Sbjct: 64 FLGNRSVI 71
>NP_919299
          Length = 498

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   VFLGER 6
           VFLGER
Sbjct: 372 VFLGER 377
>NP_659502
          Length = 469

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VFLGERS 7
           +FLGER+
Sbjct: 214 IFLGERA 220
>NP_001230064
          Length = 406

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VFLGERS 7
           +FLGER+
Sbjct: 151 IFLGERA 157
>NP_001075949
          Length = 496

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VFLGERSV 8
           + LGERSV
Sbjct: 480 IHLGERSV 487
>NP_001248772
          Length = 496

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VFLGERSV 8
           + LGERSV
Sbjct: 480 IHLGERSV 487
>NP_001230065
          Length = 362

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VFLGERS 7
           +FLGER+
Sbjct: 107 IFLGERA 113
>XP_006725158
          Length = 496

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VFLGERSV 8
           + LGERSV
Sbjct: 480 IHLGERSV 487
>XP_006719831
          Length = 479

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   VFLGER 6
           VFLGER
Sbjct: 353 VFLGER 358
>XP_006718547
          Length = 445

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VFLGERS 7
           +FLGER+
Sbjct: 190 IFLGERA 196
>XP_006716771
          Length = 496

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VFLGERSV 8
           + LGERSV
Sbjct: 480 IHLGERSV 487
>NP_001091980
          Length = 445

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VFLGERS 7
           +FLGER+
Sbjct: 190 IFLGERA 196
>NP_055604
          Length = 536

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VFLGERSV 8
           + LGERSV
Sbjct: 520 IHLGERSV 527
>NP_008835
          Length = 4128

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    FLGERSV 8
            F+GER+V
Sbjct: 1270 FIGERTV 1276
>NP_001075109
          Length = 4097

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    FLGERSV 8
            F+GER+V
Sbjct: 1270 FIGERTV 1276
>NP_002542
          Length = 321

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VFLGERSV 8
           +FLG RS+
Sbjct: 313 IFLGRRSL 320
>NP_001138502
          Length = 1692

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    FLGERSVL 9
            FLG+ SVL
Sbjct: 1057 FLGKHSVL 1064
>NP_060371
          Length = 816

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VFLGERS 7
           VFLG RS
Sbjct: 756 VFLGRRS 762
>XP_005260860
          Length = 455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VFLGERSV 8
          VFL E+SV
Sbjct: 63 VFLNEKSV 70
>XP_005260859
          Length = 545

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VFLGERSV 8
          VFL E+SV
Sbjct: 63 VFLNEKSV 70
>XP_005260858
          Length = 643

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VFLGERSV 8
          VFL E+SV
Sbjct: 63 VFLNEKSV 70
>XP_005260857
          Length = 677

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VFLGERSV 8
          VFL E+SV
Sbjct: 63 VFLNEKSV 70
>XP_006723671
          Length = 684

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VFLGERSV 8
          VFL E+SV
Sbjct: 63 VFLNEKSV 70
>XP_006723670
          Length = 711

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VFLGERSV 8
          VFL E+SV
Sbjct: 63 VFLNEKSV 70
>XP_005260854
          Length = 711

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VFLGERSV 8
          VFL E+SV
Sbjct: 63 VFLNEKSV 70
>XP_006723669
          Length = 718

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VFLGERSV 8
          VFL E+SV
Sbjct: 63 VFLNEKSV 70
>XP_005253963
          Length = 748

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VFLGERS 7
           VFLG RS
Sbjct: 688 VFLGRRS 694
>XP_005253962
          Length = 870

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VFLGERS 7
           VFLG RS
Sbjct: 810 VFLGRRS 816
>XP_006719124
          Length = 1047

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FLGERSV 8
           ++GERSV
Sbjct: 964 YIGERSV 970
>XP_005253405
          Length = 1463

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FLGERSV 8
           ++GERSV
Sbjct: 964 YIGERSV 970
>NP_001137291
          Length = 888

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VFLGERS 7
           VFLG RS
Sbjct: 828 VFLGRRS 834
>NP_071927
          Length = 711

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VFLGERSV 8
          VFL E+SV
Sbjct: 63 VFLNEKSV 70
>NP_777601
          Length = 1453

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FLGERSV 8
           ++GERSV
Sbjct: 954 YIGERSV 960
>NP_055519
          Length = 925

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VFLGERS 7
           +F GERS
Sbjct: 755 IFCGERS 761
>NP_689845
          Length = 976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   FLGERS 7
           F+GERS
Sbjct: 425 FMGERS 430
>NP_001178249
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FLGERSV 8
           FLG+RS+
Sbjct: 330 FLGDRSL 336
>NP_001265560
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FLGERSV 8
           FLG+RS+
Sbjct: 330 FLGDRSL 336
>NP_055238
          Length = 1163

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  FLGERSV 8
          F+G+RS+
Sbjct: 65 FIGDRSI 71
>NP_001178250
          Length = 402

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FLGERSV 8
           FLG+RS+
Sbjct: 344 FLGDRSL 350
>NP_071423
          Length = 686

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GERSVL 9
           GERSVL
Sbjct: 608 GERSVL 613
>NP_068712
          Length = 473

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FLGERSV 8
           FLG+RS+
Sbjct: 415 FLGDRSL 421
>NP_000805
          Length = 473

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FLGERSV 8
           FLG+RS+
Sbjct: 415 FLGDRSL 421
>XP_006718329
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GERSVL 9
           GERSVL
Sbjct: 390 GERSVL 395
>XP_006718328
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GERSVL 9
           GERSVL
Sbjct: 390 GERSVL 395
>XP_006714730
          Length = 338

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GERSVL 9
           GERSVL
Sbjct: 235 GERSVL 240
>XP_005272100
          Length = 658

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GERSVL 9
           GERSVL
Sbjct: 580 GERSVL 585
>XP_005248564
          Length = 2342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    LGERSV 8
            LGERSV
Sbjct: 1146 LGERSV 1151
>XP_006714650
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  FLGERSV 8
          F+G+RS+
Sbjct: 65 FIGDRSI 71
>XP_005272020
          Length = 1163

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  FLGERSV 8
          F+G+RS+
Sbjct: 65 FIGDRSI 71
>XP_005244872
          Length = 961

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VFLGERS 7
           +F GERS
Sbjct: 791 IFCGERS 797
>NP_005900
          Length = 2468

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    LGERSV 8
            LGERSV
Sbjct: 1272 LGERSV 1277
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.153    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 471,734
Number of extensions: 520
Number of successful extensions: 74
Number of sequences better than 10000.0: 74
Number of HSP's gapped: 74
Number of HSP's successfully gapped: 74
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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