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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     390
NP_001075109                                                           20     390
NP_006505                                                              16    5631
XP_005260020                                                           16    5631
XP_005265428                                                           16    5631
NP_060152                                                              16    5631
NP_005948                                                              15    9605
NP_699197                                                              15    9605
XP_005263520                                                           15    9605
XP_005263519                                                           15    9605
XP_005263518                                                           15    9605
XP_005263517                                                           15    9605
XP_005263515                                                           15    9605

>NP_008835
          Length = 4128

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    VFESLVIYM 9
            VFE+LVIYM
Sbjct: 1100 VFEALVIYM 1108



>NP_001075109
          Length = 4097

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    VFESLVIYM 9
            VFE+LVIYM
Sbjct: 1100 VFEALVIYM 1108



>NP_006505
          Length = 1956

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    FESLVIYM 9
            FES +I+M
Sbjct: 1154 FESFIIFM 1161



>XP_005260020
          Length = 509

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  FESLVIY 8
          FE LVIY
Sbjct: 62 FEKLVIY 68



>XP_005265428
          Length = 1959

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    FESLVIYM 9
            FES +I+M
Sbjct: 1157 FESFIIFM 1164



>NP_060152
          Length = 509

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  FESLVIY 8
          FE LVIY
Sbjct: 62 FEKLVIY 68



>NP_005948
          Length = 656

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VFESLVIYM 9
           VFE  V+Y+
Sbjct: 431 VFEVFVLYL 439



>NP_699197
          Length = 3571

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    FESLVIY 8
            FES +IY
Sbjct: 3259 FESTIIY 3265



>XP_005263520
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VFESLVIYM 9
           VFE  V+Y+
Sbjct: 349 VFEVFVLYL 357



>XP_005263519
          Length = 656

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VFESLVIYM 9
           VFE  V+Y+
Sbjct: 431 VFEVFVLYL 439



>XP_005263518
          Length = 656

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VFESLVIYM 9
           VFE  V+Y+
Sbjct: 431 VFEVFVLYL 439



>XP_005263517
          Length = 656

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VFESLVIYM 9
           VFE  V+Y+
Sbjct: 431 VFEVFVLYL 439



>XP_005263515
          Length = 697

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VFESLVIYM 9
           VFE  V+Y+
Sbjct: 472 VFEVFVLYL 480



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.342    0.152    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 349,891
Number of extensions: 722
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)