Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001257436                                                           18    1935
NP_001257437                                                           18    1935
NP_006281                                                              18    1935
XP_006715618                                                           18    1935
XP_005267176                                                           18    1935
NP_001234923                                                           17    4312
NP_001193954                                                           17    4312
NP_001193955                                                           17    4312
NP_002689                                                              17    4312
XP_005259069                                                           17    4312
XP_005259068                                                           17    4312
XP_005259067                                                           17    4312
XP_005259066                                                           17    4312
NP_000171                                                              16    7355
NP_001258636                                                           15    9605
NP_054879                                                              15    9605
NP_001013649                                                           15    9605
XP_006724707                                                           15    9605
>NP_001257436
          Length = 790

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   EASSSLST 9
           EASSSLST
Sbjct: 546 EASSSLST 553
>NP_001257437
          Length = 790

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   EASSSLST 9
           EASSSLST
Sbjct: 546 EASSSLST 553
>NP_006281
          Length = 790

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   EASSSLST 9
           EASSSLST
Sbjct: 546 EASSSLST 553
>XP_006715618
          Length = 577

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   EASSSLST 9
           EASSSLST
Sbjct: 333 EASSSLST 340
>XP_005267176
          Length = 790

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   EASSSLST 9
           EASSSLST
Sbjct: 546 EASSSLST 553
>NP_001234923
          Length = 400

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   EASSSLST 9
           +ASSSLST
Sbjct: 375 QASSSLST 382
>NP_001193954
          Length = 479

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   EASSSLST 9
           +ASSSLST
Sbjct: 391 QASSSLST 398
>NP_001193955
          Length = 467

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   EASSSLST 9
           +ASSSLST
Sbjct: 391 QASSSLST 398
>NP_002689
          Length = 463

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   EASSSLST 9
           +ASSSLST
Sbjct: 375 QASSSLST 382
>XP_005259069
          Length = 416

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   EASSSLST 9
           +ASSSLST
Sbjct: 391 QASSSLST 398
>XP_005259068
          Length = 416

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   EASSSLST 9
           +ASSSLST
Sbjct: 391 QASSSLST 398
>XP_005259067
          Length = 586

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   EASSSLST 9
           +ASSSLST
Sbjct: 375 QASSSLST 382
>XP_005259066
          Length = 602

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   EASSSLST 9
           +ASSSLST
Sbjct: 391 QASSSLST 398
>NP_000171
          Length = 1103

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   VEASSSLST 9
           VEA  SLST
Sbjct: 195 VEAGRSLST 203
>NP_001258636
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VEASSSLS 8
           VEASSS S
Sbjct: 115 VEASSSYS 122
>NP_054879
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VEASSSLS 8
           VEASSS S
Sbjct: 158 VEASSSYS 165
>NP_001013649
          Length = 1225

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   EASSSLST 9
           EAS SLST
Sbjct: 208 EASLSLST 215
>XP_006724707
          Length = 1337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   EASSSLST 9
           EAS SLST
Sbjct: 320 EASLSLST 327
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.272   0.0868    0.170 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 255,360
Number of extensions: 231
Number of successful extensions: 18
Number of sequences better than 10000.0: 18
Number of HSP's gapped: 18
Number of HSP's successfully gapped: 18
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.5 bits)
S2: 28 (15.4 bits)

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