Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002515                                                              21     229
NP_001159635                                                           17    2528
NP_001159632                                                           17    2528
NP_001159634                                                           17    2528
NP_001159633                                                           17    2528
NP_055399                                                              17    2528
XP_006716110                                                           17    2528
XP_005250542                                                           17    2528
XP_006716109                                                           17    2528
NP_060120                                                              17    2528
NP_004976                                                              17    3301
NP_203524                                                              17    3301
XP_006719132                                                           17    3301
NP_060626                                                              16    5631
NP_056193                                                              16    5631
NP_037407                                                              16    5631
NP_001243112                                                           16    5631
NP_001243111                                                           16    5631
XP_006721247                                                           16    5631
XP_006721246                                                           16    5631
XP_006721245                                                           16    5631
XP_006721244                                                           16    5631
NP_078817                                                              16    7355
XP_006721506                                                           16    7355
XP_005256500                                                           16    7355
XP_006721505                                                           16    7355
XP_006721504                                                           16    7355
XP_006718032                                                           16    7355
NP_998762                                                              16    7355
NP_597677                                                              15    9605
NP_055313                                                              15    9605
NP_056199                                                              15    9605
NP_003109                                                              15    9605
NP_110394                                                              15    9605
NP_001276834                                                           15    9605
NP_055154                                                              15    9605
NP_653267                                                              15    9605
NP_006536                                                              15    9605
NP_002283                                                              15    9605
NP_065961                                                              15    9605
NP_001158137                                                           15    9605
XP_005252305                                                           15    9605
XP_005252303                                                           15    9605
XP_006717357                                                           15    9605
XP_005252304                                                           15    9605
XP_005252302                                                           15    9605
XP_005252301                                                           15    9605
XP_005252300                                                           15    9605
XP_006717159                                                           15    9605
XP_006716135                                                           15    9605
XP_006716134                                                           15    9605
XP_005248340                                                           15    9605
XP_005248339                                                           15    9605
XP_005248337                                                           15    9605
XP_006714531                                                           15    9605
XP_006714530                                                           15    9605
XP_005247421                                                           15    9605
XP_005265184                                                           15    9605
XP_006713000                                                           15    9605
XP_005264865                                                           15    9605
XP_005264863                                                           15    9605
>NP_002515
          Length = 189

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VDTKQAHKL 9
           VDTKQAH+L
Sbjct: 125 VDTKQAHEL 133
>NP_001159635
          Length = 1090

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VDTKQAHK 8
           VDT+ AHK
Sbjct: 266 VDTEDAHK 273
>NP_001159632
          Length = 1374

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VDTKQAHK 8
           VDT+ AHK
Sbjct: 266 VDTEDAHK 273
>NP_001159634
          Length = 1253

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VDTKQAHK 8
           VDT+ AHK
Sbjct: 266 VDTEDAHK 273
>NP_001159633
          Length = 1296

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VDTKQAHK 8
           VDT+ AHK
Sbjct: 266 VDTEDAHK 273
>NP_055399
          Length = 468

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DTKQAHKL 9
           D K+AHKL
Sbjct: 367 DKKEAHKL 374
>XP_006716110
          Length = 1141

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VDTKQAHK 8
           VDT+ AHK
Sbjct: 266 VDTEDAHK 273
>XP_005250542
          Length = 1357

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VDTKQAHK 8
           VDT+ AHK
Sbjct: 266 VDTEDAHK 273
>XP_006716109
          Length = 1365

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VDTKQAHK 8
           VDT+ AHK
Sbjct: 266 VDTEDAHK 273
>NP_060120
          Length = 1098

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VDTKQAHK 8
           VDT+ AHK
Sbjct: 266 VDTEDAHK 273
>NP_004976
          Length = 188

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   VDTKQAHKL 9
           VDTKQA  L
Sbjct: 125 VDTKQAQDL 133
>NP_203524
          Length = 189

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   VDTKQAHKL 9
           VDTKQA  L
Sbjct: 125 VDTKQAQDL 133
>XP_006719132
          Length = 189

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   VDTKQAHKL 9
           VDTKQA  L
Sbjct: 125 VDTKQAQDL 133
>NP_060626
          Length = 4363

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VDTKQAHKL 9
            +D K +HKL
Sbjct: 3380 LDNKSSHKL 3388
>NP_056193
          Length = 4388

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VDTKQAHKL 9
            +D K +HKL
Sbjct: 3405 LDNKSSHKL 3413
>NP_037407
          Length = 2663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    DTKQAHK 8
            DTK+ HK
Sbjct: 1061 DTKEKHK 1067
>NP_001243112
          Length = 2663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    DTKQAHK 8
            DTK+ HK
Sbjct: 1061 DTKEKHK 1067
>NP_001243111
          Length = 2663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    DTKQAHK 8
            DTK+ HK
Sbjct: 1061 DTKEKHK 1067
>XP_006721247
          Length = 2564

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   DTKQAHK 8
           DTK+ HK
Sbjct: 962 DTKEKHK 968
>XP_006721246
          Length = 2620

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    DTKQAHK 8
            DTK+ HK
Sbjct: 1018 DTKEKHK 1024
>XP_006721245
          Length = 2629

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    DTKQAHK 8
            DTK+ HK
Sbjct: 1027 DTKEKHK 1033
>XP_006721244
          Length = 2629

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    DTKQAHK 8
            DTK+ HK
Sbjct: 1027 DTKEKHK 1033
>NP_078817
          Length = 689

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   DTKQAHKL 9
           DTK  H+L
Sbjct: 381 DTKDEHRL 388
>XP_006721506
          Length = 410

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TKQAHKL 9
           TKQAH++
Sbjct: 217 TKQAHEV 223
>XP_005256500
          Length = 458

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TKQAHKL 9
           TKQAH++
Sbjct: 265 TKQAHEV 271
>XP_006721505
          Length = 492

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TKQAHKL 9
           TKQAH++
Sbjct: 299 TKQAHEV 305
>XP_006721504
          Length = 492

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TKQAHKL 9
           TKQAH++
Sbjct: 299 TKQAHEV 305
>XP_006718032
          Length = 619

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   DTKQAHKL 9
           DTK  H+L
Sbjct: 381 DTKDEHRL 388
>NP_998762
          Length = 492

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TKQAHKL 9
           TKQAH++
Sbjct: 299 TKQAHEV 305
>NP_597677
          Length = 2804

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDTKQAH 7
           VDT +AH
Sbjct: 928 VDTNKAH 934
>NP_055313
          Length = 696

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2   DTKQAHK 8
           D KQ HK
Sbjct: 198 DVKQQHK 204
>NP_056199
          Length = 2697

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDTKQAH 7
           VDT +AH
Sbjct: 928 VDTNKAH 934
>NP_003109
          Length = 303

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TKQAHKL 9
           TK+ HKL
Sbjct: 135 TKKGHKL 141
>NP_110394
          Length = 1439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    DTKQAHKL 9
            D +QAH L
Sbjct: 1426 DLRQAHSL 1433
>NP_001276834
          Length = 832

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VDTKQAHKL 9
           V+T +AH+L
Sbjct: 209 VETLEAHQL 217
>NP_055154
          Length = 866

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VDTKQAHKL 9
           V+T +AH+L
Sbjct: 209 VETLEAHQL 217
>NP_653267
          Length = 4753

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    VDTKQAHK 8
            +  KQAHK
Sbjct: 3016 IGDKQAHK 3023
>NP_006536
          Length = 380

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   TKQAHK 8
           TKQ HK
Sbjct: 269 TKQGHK 274
>NP_002283
          Length = 1798

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1    VDTKQAHK 8
            +DT Q H+
Sbjct: 1150 IDTPQCHR 1157
>NP_065961
          Length = 1934

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2    DTKQAHKL 9
            DTK+ H++
Sbjct: 1557 DTKERHRV 1564
>NP_001158137
          Length = 1950

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2    DTKQAHKL 9
            DTK+ H++
Sbjct: 1557 DTKERHRV 1564
>XP_005252305
          Length = 1407

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    DTKQAHKL 9
            D +QAH L
Sbjct: 1394 DLRQAHSL 1401
>XP_005252303
          Length = 1439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    DTKQAHKL 9
            D +QAH L
Sbjct: 1426 DLRQAHSL 1433
>XP_006717357
          Length = 1439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    DTKQAHKL 9
            D +QAH L
Sbjct: 1426 DLRQAHSL 1433
>XP_005252304
          Length = 1439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    DTKQAHKL 9
            D +QAH L
Sbjct: 1426 DLRQAHSL 1433
>XP_005252302
          Length = 1439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    DTKQAHKL 9
            D +QAH L
Sbjct: 1426 DLRQAHSL 1433
>XP_005252301
          Length = 1439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    DTKQAHKL 9
            D +QAH L
Sbjct: 1426 DLRQAHSL 1433
>XP_005252300
          Length = 1439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    DTKQAHKL 9
            D +QAH L
Sbjct: 1426 DLRQAHSL 1433
>XP_006717159
          Length = 2293

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2    DTKQAH 7
            D KQAH
Sbjct: 1580 DAKQAH 1585
>XP_006716135
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   DTKQAHKL 9
           DTK+ H++
Sbjct: 341 DTKERHRV 348
>XP_006716134
          Length = 1847

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2    DTKQAHKL 9
            DTK+ H++
Sbjct: 1454 DTKERHRV 1461
>XP_005248340
          Length = 2265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDTKQAH 7
           VDT +AH
Sbjct: 389 VDTNKAH 395
>XP_005248339
          Length = 2584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDTKQAH 7
           VDT +AH
Sbjct: 708 VDTNKAH 714
>XP_005248337
          Length = 2698

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDTKQAH 7
           VDT +AH
Sbjct: 928 VDTNKAH 934
>XP_006714531
          Length = 2738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDTKQAH 7
           VDT +AH
Sbjct: 928 VDTNKAH 934
>XP_006714530
          Length = 2755

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDTKQAH 7
           VDT +AH
Sbjct: 928 VDTNKAH 934
>XP_005247421
          Length = 697

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2   DTKQAHK 8
           D KQ HK
Sbjct: 198 DVKQQHK 204
>XP_005265184
          Length = 1798

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1    VDTKQAHK 8
            +DT Q H+
Sbjct: 1150 IDTPQCHR 1157
>XP_006713000
          Length = 260

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   TKQAHK 8
           TKQ HK
Sbjct: 149 TKQGHK 154
>XP_005264865
          Length = 260

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   TKQAHK 8
           TKQ HK
Sbjct: 149 TKQGHK 154
>XP_005264863
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   TKQAHK 8
           TKQ HK
Sbjct: 210 TKQGHK 215
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.122    0.327 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 589,583
Number of extensions: 825
Number of successful extensions: 61
Number of sequences better than 10000.0: 61
Number of HSP's gapped: 61
Number of HSP's successfully gapped: 61
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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