Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              17    2528
XP_006716142                                                           17    2528
XP_005250088                                                           17    2528
XP_006716141                                                           17    2528
XP_005250085                                                           17    2528
XP_006716140                                                           17    2528
XP_005250084                                                           17    2528
XP_005250083                                                           17    2528
XP_005250082                                                           17    2528
NP_055325                                                              17    3301
NP_149015                                                              17    3301
XP_006715989                                                           17    3301
NP_570853                                                              17    4312
NP_570854                                                              17    4312
NP_000453                                                              17    4312
XP_005268328                                                           17    4312
XP_006720739                                                           17    4312
XP_005268327                                                           17    4312
XP_005268326                                                           17    4312
XP_005268324                                                           17    4312
XP_005268325                                                           17    4312
XP_006720738                                                           17    4312
XP_006720737                                                           17    4312
XP_006720736                                                           17    4312
NP_064448                                                              17    4312
NP_001253972                                                           16    5631
XP_006719094                                                           16    5631
NP_001107648                                                           16    5631
NP_694939                                                              16    5631
NP_001184033                                                           16    7355
NP_005924                                                              16    7355
NP_056123                                                              16    7355
NP_061928                                                              16    7355
NP_115665                                                              16    7355
NP_001035243                                                           16    7355
NP_065897                                                              16    7355
NP_001229797                                                           16    7355
XP_006719828                                                           16    7355
XP_005271654                                                           16    7355
XP_005271653                                                           16    7355
XP_006718924                                                           16    7355
XP_006718903                                                           16    7355
XP_006718902                                                           16    7355
XP_005252890                                                           16    7355
XP_006714864                                                           16    7355
XP_005248178                                                           16    7355
NP_061824                                                              15    9605
NP_055928                                                              15    9605
NP_060224                                                              15    9605
NP_055625                                                              15    9605
NP_620636                                                              15    9605
NP_001157915                                                           15    9605
NP_073752                                                              15    9605
NP_003062                                                              15    9605
NP_056381                                                              15    9605
XP_005256619                                                           15    9605
XP_006721549                                                           15    9605
XP_006721548                                                           15    9605
XP_005256618                                                           15    9605
XP_005256617                                                           15    9605
XP_005256350                                                           15    9605
XP_006720709                                                           15    9605
XP_005254660                                                           15    9605
XP_005249256                                                           15    9605
XP_006715189                                                           15    9605
XP_006715188                                                           15    9605
XP_005248537                                                           15    9605
XP_005248536                                                           15    9605
XP_005247781                                                           15    9605
XP_006711734                                                           15    9605
XP_006711733                                                           15    9605
XP_006711732                                                           15    9605
XP_006711731                                                           15    9605
XP_006711730                                                           15    9605
XP_006711729                                                           15    9605
XP_005245695                                                           15    9605
>NP_733751
          Length = 4911

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VDSEISSSR 9
            V SEISSSR
Sbjct: 3437 VGSEISSSR 3445
>XP_006716142
          Length = 4927

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VDSEISSSR 9
            V SEISSSR
Sbjct: 3454 VGSEISSSR 3462
>XP_005250088
          Length = 4928

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VDSEISSSR 9
            V SEISSSR
Sbjct: 3454 VGSEISSSR 3462
>XP_006716141
          Length = 4959

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VDSEISSSR 9
            V SEISSSR
Sbjct: 3454 VGSEISSSR 3462
>XP_005250085
          Length = 4979

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VDSEISSSR 9
            V SEISSSR
Sbjct: 3454 VGSEISSSR 3462
>XP_006716140
          Length = 4982

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VDSEISSSR 9
            V SEISSSR
Sbjct: 3454 VGSEISSSR 3462
>XP_005250084
          Length = 4982

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VDSEISSSR 9
            V SEISSSR
Sbjct: 3454 VGSEISSSR 3462
>XP_005250083
          Length = 4982

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VDSEISSSR 9
            V SEISSSR
Sbjct: 3453 VGSEISSSR 3461
>XP_005250082
          Length = 4983

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VDSEISSSR 9
            V SEISSSR
Sbjct: 3454 VGSEISSSR 3462
>NP_055325
          Length = 4935

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    DSEISSSR 9
            DSE+SS+R
Sbjct: 3836 DSEVSSTR 3843
>NP_149015
          Length = 5142

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    DSEISSSR 9
            DSE+SS+R
Sbjct: 3836 DSEVSSTR 3843
>XP_006715989
          Length = 5148

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    DSEISSSR 9
            DSE+SS+R
Sbjct: 3836 DSEVSSTR 3843
>NP_570853
          Length = 852

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DSE SSSR
Sbjct: 183 DSEASSSR 190
>NP_570854
          Length = 872

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DSE SSSR
Sbjct: 203 DSEASSSR 210
>NP_000453
          Length = 875

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DSE SSSR
Sbjct: 206 DSEASSSR 213
>XP_005268328
          Length = 852

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DSE SSSR
Sbjct: 183 DSEASSSR 190
>XP_006720739
          Length = 852

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DSE SSSR
Sbjct: 183 DSEASSSR 190
>XP_005268327
          Length = 852

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DSE SSSR
Sbjct: 183 DSEASSSR 190
>XP_005268326
          Length = 852

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DSE SSSR
Sbjct: 183 DSEASSSR 190
>XP_005268324
          Length = 852

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DSE SSSR
Sbjct: 183 DSEASSSR 190
>XP_005268325
          Length = 852

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DSE SSSR
Sbjct: 183 DSEASSSR 190
>XP_006720738
          Length = 852

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DSE SSSR
Sbjct: 183 DSEASSSR 190
>XP_006720737
          Length = 872

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DSE SSSR
Sbjct: 203 DSEASSSR 210
>XP_006720736
          Length = 872

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DSE SSSR
Sbjct: 203 DSEASSSR 210
>NP_064448
          Length = 327

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           D EISSSR
Sbjct: 161 DREISSSR 168
>NP_001253972
          Length = 301

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  VDSEISSSR 9
          VDS ISS R
Sbjct: 16 VDSTISSGR 24
>XP_006719094
          Length = 479

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   VDSEISSSR 9
           VDS ISS R
Sbjct: 232 VDSTISSGR 240
>NP_001107648
          Length = 517

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   VDSEISSSR 9
           VDS ISS R
Sbjct: 232 VDSTISSGR 240
>NP_694939
          Length = 503

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   VDSEISSSR 9
           VDS ISS R
Sbjct: 232 VDSTISSGR 240
>NP_001184033
          Length = 3972

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VDSEISSS 8
            VDS +SSS
Sbjct: 2910 VDSSVSSS 2917
>NP_005924
          Length = 3969

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VDSEISSS 8
            VDS +SSS
Sbjct: 2907 VDSSVSSS 2914
>NP_056123
          Length = 1017

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VDSEISSS 8
           VDS ISSS
Sbjct: 776 VDSTISSS 783
>NP_061928
          Length = 1007

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DS+ISS R
Sbjct: 982 DSQISSQR 989
>NP_115665
          Length = 771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   DSEISSS 8
           DSE+SSS
Sbjct: 286 DSELSSS 292
>NP_001035243
          Length = 426

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VDSEISSS 8
           +DSE+S+S
Sbjct: 106 IDSELSTS 113
>NP_065897
          Length = 581

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VDSEISSS 8
           +DSE+S+S
Sbjct: 252 IDSELSAS 259
>NP_001229797
          Length = 590

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VDSEISSS 8
           +DSE+S+S
Sbjct: 270 IDSELSTS 277
>XP_006719828
          Length = 640

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VDSEISSS 8
           +DSE+S+S
Sbjct: 320 IDSELSTS 327
>XP_005271654
          Length = 862

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DS+ISS R
Sbjct: 837 DSQISSQR 844
>XP_005271653
          Length = 862

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DS+ISS R
Sbjct: 837 DSQISSQR 844
>XP_006718924
          Length = 969

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DS+ISS R
Sbjct: 944 DSQISSQR 951
>XP_006718903
          Length = 2673

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VDSEISSS 8
            VDS +SSS
Sbjct: 1611 VDSSVSSS 1618
>XP_006718902
          Length = 2964

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VDSEISSS 8
            VDS +SSS
Sbjct: 1902 VDSSVSSS 1909
>XP_005252890
          Length = 1019

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VDSEISSS 8
           VDS ISSS
Sbjct: 778 VDSTISSS 785
>XP_006714864
          Length = 772

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   DSEISSS 8
           DSE+SSS
Sbjct: 286 DSELSSS 292
>XP_005248178
          Length = 607

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VDSEISSS 8
           +DSE+S+S
Sbjct: 252 IDSELSAS 259
>NP_061824
          Length = 410

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  VDSEISSSR 9
          VDS ISS R
Sbjct: 31 VDSLISSGR 39
>NP_055928
          Length = 2961

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    DSEISSSR 9
            DSE+SS +
Sbjct: 2059 DSEVSSQK 2066
>NP_060224
          Length = 1440

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VDSEISSS 8
           VDSE+S S
Sbjct: 904 VDSELSPS 911
>NP_055625
          Length = 3117

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VDSEISS 7
            VDS+ISS
Sbjct: 2657 VDSQISS 2663
>NP_620636
          Length = 1009

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SEISSSR 9
           SE+SSSR
Sbjct: 378 SELSSSR 384
>NP_001157915
          Length = 1873

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    DSEISSS 8
            DS+ISSS
Sbjct: 1667 DSQISSS 1673
>NP_073752
          Length = 1460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VDSEISSSR 9
           VDS + SSR
Sbjct: 990 VDSALGSSR 998
>NP_003062
          Length = 1009

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SEISSSR 9
           SE+SSSR
Sbjct: 378 SELSSSR 384
>NP_056381
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DSE SSS+
Sbjct: 344 DSEFSSSQ 351
>XP_005256619
          Length = 1581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DSE+SS +
Sbjct: 675 DSEVSSQK 682
>XP_006721549
          Length = 1581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DSE+SS +
Sbjct: 675 DSEVSSQK 682
>XP_006721548
          Length = 1886

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DSE+SS +
Sbjct: 980 DSEVSSQK 987
>XP_005256618
          Length = 2962

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    DSEISSSR 9
            DSE+SS +
Sbjct: 2060 DSEVSSQK 2067
>XP_005256617
          Length = 2966

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    DSEISSSR 9
            DSE+SS +
Sbjct: 2060 DSEVSSQK 2067
>XP_005256350
          Length = 610

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VDSEISSS 8
           V SE+SSS
Sbjct: 578 VSSEVSSS 585
>XP_006720709
          Length = 1189

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VDSEISSSR 9
           VDS + SSR
Sbjct: 719 VDSALGSSR 727
>XP_005254660
          Length = 1460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VDSEISSSR 9
           VDS + SSR
Sbjct: 990 VDSALGSSR 998
>XP_005249256
          Length = 1054

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VDSEISSS 8
           VDSE+S S
Sbjct: 518 VDSELSPS 525
>XP_006715189
          Length = 1240

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VDSEISSS 8
           VDSE+S S
Sbjct: 904 VDSELSPS 911
>XP_006715188
          Length = 1400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VDSEISSS 8
           VDSE+S S
Sbjct: 904 VDSELSPS 911
>XP_005248537
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DSE SSS+
Sbjct: 344 DSEFSSSQ 351
>XP_005248536
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DSEISSSR 9
           DSE SSS+
Sbjct: 406 DSEFSSSQ 413
>XP_005247781
          Length = 1008

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SEISSSR 9
           SE+SSSR
Sbjct: 378 SELSSSR 384
>XP_006711734
          Length = 3136

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VDSEISS 7
            VDS+ISS
Sbjct: 2676 VDSQISS 2682
>XP_006711733
          Length = 3137

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VDSEISS 7
            VDS+ISS
Sbjct: 2677 VDSQISS 2683
>XP_006711732
          Length = 3154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VDSEISS 7
            VDS+ISS
Sbjct: 2694 VDSQISS 2700
>XP_006711731
          Length = 3157

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VDSEISS 7
            VDS+ISS
Sbjct: 2697 VDSQISS 2703
>XP_006711730
          Length = 3157

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VDSEISS 7
            VDS+ISS
Sbjct: 2697 VDSQISS 2703
>XP_006711729
          Length = 3158

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VDSEISS 7
            VDS+ISS
Sbjct: 2698 VDSQISS 2704
>XP_005245695
          Length = 3158

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VDSEISS 7
            VDS+ISS
Sbjct: 2698 VDSQISS 2704
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.288    0.106    0.227 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 443,058
Number of extensions: 863
Number of successful extensions: 76
Number of sequences better than 10000.0: 76
Number of HSP's gapped: 76
Number of HSP's successfully gapped: 76
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)

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