Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              18    1135
NP_001075109                                                           18    1135
NP_065820                                                              16    5631
NP_065920                                                              16    5631
NP_775841                                                              16    5631
XP_005247327                                                           16    5631
XP_005247326                                                           16    5631
XP_006713625                                                           16    5631
XP_005247324                                                           16    5631
XP_006713624                                                           16    5631
XP_005247323                                                           16    5631
XP_005247322                                                           16    5631
NP_000247                                                              16    7355
NP_919442                                                              16    7355
NP_588616                                                              16    7355
NP_001137160                                                           16    7355
XP_005259651                                                           16    7355
XP_005254204                                                           16    7355
XP_005254202                                                           16    7355
XP_005254201                                                           16    7355
XP_006718301                                                           16    7355
NP_001263256                                                           15    9605
NP_001263255                                                           15    9605
NP_653186                                                              15    9605
NP_945185                                                              15    9605
NP_001136175                                                           15    9605
NP_001136176                                                           15    9605
NP_001138820                                                           15    9605
NP_078887                                                              15    9605
NP_079049                                                              15    9605
NP_001263314                                                           15    9605
NP_001018099                                                           15    9605
NP_115544                                                              15    9605
XP_006721155                                                           15    9605
XP_005254720                                                           15    9605
XP_005254719                                                           15    9605
XP_005254718                                                           15    9605
XP_006718494                                                           15    9605
XP_005273799                                                           15    9605
XP_006718493                                                           15    9605
>NP_008835
          Length = 4128

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VDRSLFTLL 9
            VDRSL TLL
Sbjct: 1820 VDRSLLTLL 1828
>NP_001075109
          Length = 4097

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VDRSLFTLL 9
            VDRSL TLL
Sbjct: 1820 VDRSLLTLL 1828
>NP_065820
          Length = 1388

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    VDRSLFT 7
            +DRSL+T
Sbjct: 1301 IDRSLYT 1307
>NP_065920
          Length = 890

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VDRSLFTLL 9
           +D+SL TL+
Sbjct: 631 IDKSLLTLI 639
>NP_775841
          Length = 409

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DRSLFTLL 9
           DRS+FT L
Sbjct: 329 DRSVFTAL 336
>XP_005247327
          Length = 274

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DRSLFTLL 9
           DRS+FT L
Sbjct: 168 DRSVFTAL 175
>XP_005247326
          Length = 409

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DRSLFTLL 9
           DRS+FT L
Sbjct: 329 DRSVFTAL 336
>XP_006713625
          Length = 424

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DRSLFTLL 9
           DRS+FT L
Sbjct: 329 DRSVFTAL 336
>XP_005247324
          Length = 429

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DRSLFTLL 9
           DRS+FT L
Sbjct: 323 DRSVFTAL 330
>XP_006713624
          Length = 432

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DRSLFTLL 9
           DRS+FT L
Sbjct: 326 DRSVFTAL 333
>XP_005247323
          Length = 435

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DRSLFTLL 9
           DRS+FT L
Sbjct: 329 DRSVFTAL 336
>XP_005247322
          Length = 435

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DRSLFTLL 9
           DRS+FT L
Sbjct: 329 DRSVFTAL 336
>NP_000247
          Length = 1274

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   DRSLFTL 8
           DRS+FT+
Sbjct: 732 DRSIFTV 738
>NP_919442
          Length = 311

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VDRSLFTL 8
          VD+ LFTL
Sbjct: 68 VDQHLFTL 75
>NP_588616
          Length = 971

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RSLFTLL 9
           RSL+TLL
Sbjct: 948 RSLYTLL 954
>NP_001137160
          Length = 1054

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    RSLFTLL 9
            RSL+TLL
Sbjct: 1031 RSLYTLL 1037
>XP_005259651
          Length = 284

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VDRSLFTL 8
          VD+ LFTL
Sbjct: 68 VDQHLFTL 75
>XP_005254204
          Length = 971

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RSLFTLL 9
           RSL+TLL
Sbjct: 948 RSLYTLL 954
>XP_005254202
          Length = 971

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RSLFTLL 9
           RSL+TLL
Sbjct: 948 RSLYTLL 954
>XP_005254201
          Length = 1037

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    RSLFTLL 9
            RSL+TLL
Sbjct: 1014 RSLYTLL 1020
>XP_006718301
          Length = 1268

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   DRSLFTL 8
           DRS+FT+
Sbjct: 726 DRSIFTV 732
>NP_001263256
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   DRSLFT 7
           DRS+FT
Sbjct: 123 DRSIFT 128
>NP_001263255
          Length = 519

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   DRSLFT 7
           DRS+FT
Sbjct: 123 DRSIFT 128
>NP_653186
          Length = 553

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   DRSLFT 7
           DRS+FT
Sbjct: 123 DRSIFT 128
>NP_945185
          Length = 734

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  VDRSLFT 7
          +DRS+FT
Sbjct: 92 LDRSVFT 98
>NP_001136175
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  VDRSLFT 7
          +DRS+FT
Sbjct: 62 LDRSVFT 68
>NP_001136176
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  VDRSLFT 7
          +DRS+FT
Sbjct: 62 LDRSVFT 68
>NP_001138820
          Length = 454

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VDRSLFTLL 9
          V+RS+ TLL
Sbjct: 79 VERSVVTLL 87
>NP_078887
          Length = 982

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RSLFTLL 9
           +SLFTLL
Sbjct: 285 QSLFTLL 291
>NP_079049
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VDRSLFTLL 9
          V+RS+ TLL
Sbjct: 41 VERSVVTLL 49
>NP_001263314
          Length = 383

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RSLFTLL 9
           +SLFTLL
Sbjct: 285 QSLFTLL 291
>NP_001018099
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RSLFTLL 9
           +SLFTLL
Sbjct: 148 QSLFTLL 154
>NP_115544
          Length = 830

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   DRSLFT 7
           DRS+FT
Sbjct: 333 DRSIFT 338
>XP_006721155
          Length = 427

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VDRSLFTLL 9
          V+RS+ TLL
Sbjct: 52 VERSVVTLL 60
>XP_005254720
          Length = 772

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RSLFTLL 9
           +SLFTLL
Sbjct: 285 QSLFTLL 291
>XP_005254719
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RSLFTLL 9
           +SLFTLL
Sbjct: 148 QSLFTLL 154
>XP_005254718
          Length = 982

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RSLFTLL 9
           +SLFTLL
Sbjct: 285 QSLFTLL 291
>XP_006718494
          Length = 543

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  DRSLFT 7
          DRS+FT
Sbjct: 88 DRSIFT 93
>XP_005273799
          Length = 578

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   DRSLFT 7
           DRS+FT
Sbjct: 123 DRSIFT 128
>XP_006718493
          Length = 578

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   DRSLFT 7
           DRS+FT
Sbjct: 123 DRSIFT 128
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.339    0.152    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 384,558
Number of extensions: 528
Number of successful extensions: 40
Number of sequences better than 10000.0: 40
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 40
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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