Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              20     299
XP_005266703                                                           20     299
NP_004228                                                              18    1135
XP_005248445                                                           18    1135
NP_002635                                                              17    2528
XP_005273220                                                           17    2528
NP_003255                                                              17    4312
NP_001025051                                                           17    4312
NP_001018049                                                           17    4312
XP_005252608                                                           17    4312
XP_006717553                                                           17    4312
XP_005263250                                                           17    4312
XP_005263252                                                           17    4312
XP_005263253                                                           17    4312
XP_005263254                                                           17    4312
XP_005263251                                                           17    4312
NP_002372                                                              16    5631
XP_006712581                                                           16    7355
XP_005246132                                                           16    7355
NP_001073973                                                           16    7355
NP_001367                                                              15    9605
NP_002308                                                              15    9605
NP_001171573                                                           15    9605
NP_001269810                                                           15    9605
NP_001093587                                                           15    9605
NP_055492                                                              15    9605
NP_000866                                                              15    9605
NP_066957                                                              15    9605
NP_001092284                                                           15    9605
NP_001399                                                              15    9605
NP_055097                                                              15    9605
XP_006724959                                                           15    9605
XP_005254954                                                           15    9605
XP_005254953                                                           15    9605
XP_006720550                                                           15    9605
XP_006720549                                                           15    9605
XP_006718334                                                           15    9605
XP_005251869                                                           15    9605
XP_006716762                                                           15    9605
XP_006716758                                                           15    9605
XP_006716757                                                           15    9605
XP_006716756                                                           15    9605
XP_005251165                                                           15    9605
XP_005251164                                                           15    9605
XP_005251163                                                           15    9605
XP_006716755                                                           15    9605
XP_006716754                                                           15    9605
XP_006716753                                                           15    9605
XP_006710481                                                           15    9605
XP_006710468                                                           15    9605
XP_005270637                                                           15    9605
NP_001276                                                              15    9605
>NP_443179
          Length = 2170

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VDRADLRSY 9
            VDRAD RSY
Sbjct: 1114 VDRADFRSY 1122
>XP_005266703
          Length = 1501

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VDRADLRSY 9
           VDRAD RSY
Sbjct: 445 VDRADFRSY 453
>NP_004228
          Length = 432

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VDRADLRSY 9
           VDRAD++ Y
Sbjct: 310 VDRADIKQY 318
>XP_005248445
          Length = 417

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VDRADLRSY 9
           VDRAD++ Y
Sbjct: 295 VDRADIKQY 303
>NP_002635
          Length = 764

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VDRADLRSY 9
           VDR  +RSY
Sbjct: 669 VDRVSIRSY 677
>XP_005273220
          Length = 773

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VDRADLRSY 9
           VDR  +RSY
Sbjct: 669 VDRVSIRSY 677
>NP_003255
          Length = 784

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VDRADLRSY 9
           +D +DL+SY
Sbjct: 181 IDASDLQSY 189
>NP_001025051
          Length = 894

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DRADLRSY 9
           D ADLRS+
Sbjct: 291 DHADLRSH 298
>NP_001018049
          Length = 894

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DRADLRSY 9
           D ADLRS+
Sbjct: 291 DHADLRSH 298
>XP_005252608
          Length = 642

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  DRADLRSY 9
          D ADLRS+
Sbjct: 39 DHADLRSH 46
>XP_006717553
          Length = 725

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DRADLRSY 9
           D ADLRS+
Sbjct: 122 DHADLRSH 129
>XP_005263250
          Length = 784

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VDRADLRSY 9
           +D +DL+SY
Sbjct: 181 IDASDLQSY 189
>XP_005263252
          Length = 784

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VDRADLRSY 9
           +D +DL+SY
Sbjct: 181 IDASDLQSY 189
>XP_005263253
          Length = 784

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VDRADLRSY 9
           +D +DL+SY
Sbjct: 181 IDASDLQSY 189
>XP_005263254
          Length = 784

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VDRADLRSY 9
           +D +DL+SY
Sbjct: 181 IDASDLQSY 189
>XP_005263251
          Length = 784

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VDRADLRSY 9
           +D +DL+SY
Sbjct: 181 IDASDLQSY 189
>NP_002372
          Length = 486

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VDRADLRS 8
           VDRAD+ S
Sbjct: 222 VDRADMAS 229
>XP_006712581
          Length = 1047

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VDRADLRSY 9
           V  ADLRS+
Sbjct: 837 VSEADLRSH 845
>XP_005246132
          Length = 1047

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VDRADLRSY 9
           V  ADLRS+
Sbjct: 837 VSEADLRSH 845
>NP_001073973
          Length = 1052

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VDRADLRSY 9
           V  ADLRS+
Sbjct: 842 VSEADLRSH 850
>NP_001367
          Length = 4646

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VDRADLRSY 9
            VD+ +LR Y
Sbjct: 2884 VDQEELRDY 2892
>NP_002308
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RADLRSY 9
           RAD+R Y
Sbjct: 251 RADVRDY 257
>NP_001171573
          Length = 187

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  RADLRSY 9
          RAD+R Y
Sbjct: 21 RADVRDY 27
>NP_001269810
          Length = 1188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDRADLR 7
           +DR DLR
Sbjct: 251 IDRPDLR 257
>NP_001093587
          Length = 1349

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDRADLR 7
           +DR DLR
Sbjct: 443 IDRPDLR 449
>NP_055492
          Length = 1163

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDRADLR 7
           +DR DLR
Sbjct: 251 IDRPDLR 257
>NP_000866
          Length = 1367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    RADLRSY 9
            R DL+SY
Sbjct: 1084 RGDLKSY 1090
>NP_066957
          Length = 153

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  RADLRSY 9
          R DLR+Y
Sbjct: 50 RVDLRNY 56
>NP_001092284
          Length = 473

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VDRADL 6
           +DRADL
Sbjct: 215 IDRADL 220
>NP_001399
          Length = 2923

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VDRADLRSY 9
           VDR ++ SY
Sbjct: 355 VDREEVESY 363
>NP_055097
          Length = 413

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VDRADLRSY 9
           ++RA+ RSY
Sbjct: 140 LERAERRSY 148
>XP_006724959
          Length = 153

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  RADLRSY 9
          R DLR+Y
Sbjct: 50 RVDLRNY 56
>XP_005254954
          Length = 1246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RADLRSY 9
           R DL+SY
Sbjct: 963 RGDLKSY 969
>XP_005254953
          Length = 1366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    RADLRSY 9
            R DL+SY
Sbjct: 1083 RGDLKSY 1089
>XP_006720550
          Length = 1373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    RADLRSY 9
            R DL+SY
Sbjct: 1090 RGDLKSY 1096
>XP_006720549
          Length = 1374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    RADLRSY 9
            R DL+SY
Sbjct: 1091 RGDLKSY 1097
>XP_006718334
          Length = 123

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  RADLRSY 9
          R DLR+Y
Sbjct: 50 RVDLRNY 56
>XP_005251869
          Length = 303

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VDRADLRSY 9
          ++RA+ RSY
Sbjct: 30 LERAERRSY 38
>XP_006716762
          Length = 1571

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDRADLR 7
           +DR DLR
Sbjct: 487 IDRPDLR 493
>XP_006716758
          Length = 1269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDRADLR 7
           +DR DLR
Sbjct: 487 IDRPDLR 493
>XP_006716757
          Length = 1356

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDRADLR 7
           +DR DLR
Sbjct: 251 IDRPDLR 257
>XP_006716756
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDRADLR 7
           +DR DLR
Sbjct: 487 IDRPDLR 493
>XP_005251165
          Length = 1532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDRADLR 7
           +DR DLR
Sbjct: 483 IDRPDLR 489
>XP_005251164
          Length = 1552

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDRADLR 7
           +DR DLR
Sbjct: 443 IDRPDLR 449
>XP_005251163
          Length = 1574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDRADLR 7
           +DR DLR
Sbjct: 443 IDRPDLR 449
>XP_006716755
          Length = 1634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDRADLR 7
           +DR DLR
Sbjct: 487 IDRPDLR 493
>XP_006716754
          Length = 1655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDRADLR 7
           +DR DLR
Sbjct: 487 IDRPDLR 493
>XP_006716753
          Length = 1656

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VDRADLR 7
           +DR DLR
Sbjct: 487 IDRPDLR 493
>XP_006710481
          Length = 542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   RADLRS 8
           RADLRS
Sbjct: 463 RADLRS 468
>XP_006710468
          Length = 2916

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VDRADLRSY 9
           VDR ++ SY
Sbjct: 355 VDREEVESY 363
>XP_005270637
          Length = 2921

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VDRADLRSY 9
           VDR ++ SY
Sbjct: 355 VDREEVESY 363
>NP_001276
          Length = 914

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VDRADLRS 8
           VD+ DL+S
Sbjct: 847 VDKVDLKS 854
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.138    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 443,788
Number of extensions: 708
Number of successful extensions: 52
Number of sequences better than 10000.0: 52
Number of HSP's gapped: 52
Number of HSP's successfully gapped: 52
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package