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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              19     869
NP_001888                                                              16    5631
NP_004318                                                              16    5631
NP_001122302                                                           15    9605
NP_001122303                                                           15    9605
NP_001158302                                                           15    9605
NP_001122301                                                           15    9605
NP_001729                                                              15    9605
NP_005338                                                              15    9605
NP_001158220                                                           15    9605
NP_001158219                                                           15    9605
NP_009142                                                              15    9605
NP_001158218                                                           15    9605
NP_054722                                                              15    9605
NP_001163935                                                           15    9605
NP_002586                                                              15    9605
XP_005260155                                                           15    9605
XP_005256326                                                           15    9605
XP_006719507                                                           15    9605
XP_006719091                                                           15    9605
XP_006719090                                                           15    9605
NP_115809                                                              15    9605

>NP_002430
          Length = 1137

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VDIKGSQTI 9
           VDIKGSQ I
Sbjct: 481 VDIKGSQII 489



>NP_001888
          Length = 2322

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    VDIKGSQTI 9
            + +KGSQT+
Sbjct: 1582 LSLKGSQTL 1590



>NP_004318
          Length = 1271

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   VDIKGSQTI 9
           ++++GSQT+
Sbjct: 964 IELEGSQTL 972



>NP_001122302
          Length = 261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VDIKGSQT 8
          VDIK S+T
Sbjct: 32 VDIKTSET 39



>NP_001122303
          Length = 261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VDIKGSQT 8
          VDIK S+T
Sbjct: 32 VDIKTSET 39



>NP_001158302
          Length = 261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VDIKGSQT 8
          VDIK S+T
Sbjct: 32 VDIKTSET 39



>NP_001122301
          Length = 261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VDIKGSQT 8
          VDIK S+T
Sbjct: 32 VDIKTSET 39



>NP_001729
          Length = 261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VDIKGSQT 8
          VDIK S+T
Sbjct: 32 VDIKTSET 39



>NP_005338
          Length = 654

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   VDIKGSQT 8
           VDI G QT
Sbjct: 130 VDIGGGQT 137



>NP_001158220
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VDIKGSQTI 9
           +DI+G Q++
Sbjct: 302 IDIQGQQSL 310



>NP_001158219
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VDIKGSQTI 9
           +DI+G Q++
Sbjct: 302 IDIQGQQSL 310



>NP_009142
          Length = 392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VDIKGSQTI 9
           +DI+G Q++
Sbjct: 302 IDIQGQQSL 310



>NP_001158218
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VDIKGSQTI 9
           +DI+G Q++
Sbjct: 302 IDIQGQQSL 310



>NP_054722
          Length = 1227

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KGSQTI 9
           KGSQT+
Sbjct: 446 KGSQTV 451



>NP_001163935
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  IKGSQTI 9
          ++GSQTI
Sbjct: 12 LRGSQTI 18



>NP_002586
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  IKGSQTI 9
          ++GSQTI
Sbjct: 12 LRGSQTI 18



>XP_005260155
          Length = 556

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   VDIKGSQ 7
           +D+KG Q
Sbjct: 187 IDVKGEQ 193



>XP_005256326
          Length = 1192

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KGSQTI 9
           KGSQT+
Sbjct: 411 KGSQTV 416



>XP_006719507
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  IKGSQTI 9
          ++GSQTI
Sbjct: 12 LRGSQTI 18



>XP_006719091
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VDIKGSQTI 9
           +DI+G Q++
Sbjct: 302 IDIQGQQSL 310



>XP_006719090
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VDIKGSQTI 9
           +DI+G Q++
Sbjct: 302 IDIQGQQSL 310



>NP_115809
          Length = 665

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   VDIKGSQ 7
           +D+KG Q
Sbjct: 296 IDVKGEQ 302



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.133    0.336 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 519,378
Number of extensions: 833
Number of successful extensions: 22
Number of sequences better than 10000.0: 22
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)