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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000312                                                              20     509
NP_001156602                                                           17    3301
NP_001030008                                                           17    3301
NP_663629                                                              17    3301
NP_001156593                                                           17    3301
NP_001156594                                                           17    3301
NP_001257903                                                           17    3301
XP_006721269                                                           17    3301
NP_001161067                                                           17    4312
NP_055139                                                              17    4312
NP_060251                                                              16    7355
XP_005249849                                                           16    7355
NP_861420                                                              15    9605
NP_005516                                                              15    9605
NP_001193670                                                           15    9605
NP_055919                                                              15    9605
NP_079272                                                              15    9605
XP_005273907                                                           15    9605
XP_005273906                                                           15    9605
XP_006713858                                                           15    9605

>NP_000312
          Length = 928

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   CALEVVMA 9
           CALEVVMA
Sbjct: 489 CALEVVMA 496



>NP_001156602
          Length = 261

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   CALEVVM 8
           CALEVV+
Sbjct: 138 CALEVVL 144



>NP_001030008
          Length = 236

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   CALEVVM 8
           CALEVV+
Sbjct: 161 CALEVVL 167



>NP_663629
          Length = 284

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   CALEVVM 8
           CALEVV+
Sbjct: 161 CALEVVL 167



>NP_001156593
          Length = 251

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   CALEVVM 8
           CALEVV+
Sbjct: 161 CALEVVL 167



>NP_001156594
          Length = 228

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   CALEVVM 8
           CALEVV+
Sbjct: 138 CALEVVL 144



>NP_001257903
          Length = 5121

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    VCALEVVMA 9
            VC LEV+ A
Sbjct: 4484 VCKLEVIFA 4492



>XP_006721269
          Length = 5138

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    VCALEVVMA 9
            VC LEV+ A
Sbjct: 4501 VCKLEVIFA 4509



>NP_001161067
          Length = 394

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VCALEVVMA 9
           +CAL ++MA
Sbjct: 159 MCALGIIMA 167



>NP_055139
          Length = 382

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VCALEVVMA 9
           +CAL ++MA
Sbjct: 159 MCALGIIMA 167



>NP_060251
          Length = 490

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   CALEVVMA 9
           C L++VMA
Sbjct: 289 CTLDMVMA 296



>XP_005249849
          Length = 490

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   CALEVVMA 9
           C L++VMA
Sbjct: 289 CTLDMVMA 296



>NP_861420
          Length = 292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   CALEVV 7
           CALE++
Sbjct: 241 CALEII 246



>NP_005516
          Length = 292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   CALEVV 7
           CALE++
Sbjct: 241 CALEII 246



>NP_001193670
          Length = 292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   CALEVV 7
           CALE++
Sbjct: 241 CALEII 246



>NP_055919
          Length = 1938

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   CALEVVM 8
           C+LEV++
Sbjct: 811 CSLEVIL 817



>NP_079272
          Length = 751

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VCALEVVMA 9
           +C LE +MA
Sbjct: 643 LCILEPIMA 651



>XP_005273907
          Length = 1930

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   CALEVVM 8
           C+LEV++
Sbjct: 811 CSLEVIL 817



>XP_005273906
          Length = 1932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   CALEVVM 8
           C+LEV++
Sbjct: 811 CSLEVIL 817



>XP_006713858
          Length = 712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VCALEVVMA 9
           +C LE +MA
Sbjct: 604 LCILEPIMA 612



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.338    0.134    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 422,006
Number of extensions: 416
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)