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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_060952                                                              17    4312
NP_757374                                                              17    4312
NP_001171618                                                           17    4312
NP_000750                                                              17    4312
NP_757373                                                              17    4312
XP_006716629                                                           17    4312
XP_006716626                                                           17    4312
XP_005250982                                                           17    4312
NP_001070666                                                           16    5631
NP_076990                                                              16    5631
NP_001243000                                                           16    7355
XP_005257603                                                           16    7355
XP_006722058                                                           16    7355
XP_005257602                                                           16    7355
NP_115598                                                              15    9605

>NP_060952
          Length = 1129

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  ASRLQSFS 9
          ASRL SFS
Sbjct: 5  ASRLSSFS 12



>NP_757374
          Length = 171

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VASRLQSF 8
           VAS LQSF
Sbjct: 150 VASHLQSF 157



>NP_001171618
          Length = 168

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VASRLQSF 8
           VAS LQSF
Sbjct: 147 VASHLQSF 154



>NP_000750
          Length = 207

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VASRLQSF 8
           VAS LQSF
Sbjct: 186 VASHLQSF 193



>NP_757373
          Length = 204

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VASRLQSF 8
           VAS LQSF
Sbjct: 183 VASHLQSF 190



>XP_006716629
          Length = 1075

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  ASRLQSFS 9
          ASRL SFS
Sbjct: 5  ASRLSSFS 12



>XP_006716626
          Length = 1132

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  ASRLQSFS 9
          ASRL SFS
Sbjct: 5  ASRLSSFS 12



>XP_005250982
          Length = 1132

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  ASRLQSFS 9
          ASRL SFS
Sbjct: 5  ASRLSSFS 12



>NP_001070666
          Length = 839

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   VASRLQSFS 9
           VAS L+SFS
Sbjct: 648 VASALRSFS 656



>NP_076990
          Length = 839

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   VASRLQSFS 9
           VAS L+SFS
Sbjct: 648 VASALRSFS 656



>NP_001243000
          Length = 5207

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    SRLQSFS 9
            SRLQ+FS
Sbjct: 3823 SRLQNFS 3829



>XP_005257603
          Length = 5256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    SRLQSFS 9
            SRLQ+FS
Sbjct: 3872 SRLQNFS 3878



>XP_006722058
          Length = 5256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    SRLQSFS 9
            SRLQ+FS
Sbjct: 3872 SRLQNFS 3878



>XP_005257602
          Length = 5256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    SRLQSFS 9
            SRLQ+FS
Sbjct: 3872 SRLQNFS 3878



>NP_115598
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VASRLQSFS 9
           +ASR Q FS
Sbjct: 516 LASRTQQFS 524



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.119    0.278 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 314,162
Number of extensions: 459
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)