Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           20     390
NP_000242                                                              20     390
XP_005264389                                                           20     390
NP_060016                                                              17    4312
NP_001036212                                                           17    4312
NP_001036194                                                           17    4312
NP_005239                                                              17    4312
XP_006710515                                                           17    4312
NP_000042                                                              16    5631
NP_001073982                                                           16    5631
NP_031392                                                              16    5631
NP_001007268                                                           16    5631
NP_001182570                                                           16    5631
XP_006718908                                                           16    5631
XP_005271621                                                           16    5631
XP_006718907                                                           16    5631
XP_006718906                                                           16    5631
XP_005271619                                                           16    5631
XP_005271618                                                           16    5631
XP_005269338                                                           16    5631
XP_005269337                                                           16    5631
XP_005246452                                                           16    5631
XP_006712432                                                           16    5631
XP_005246450                                                           16    5631
XP_005246449                                                           16    5631
NP_996763                                                              16    5631
NP_001267                                                              16    7355
NP_004035                                                              16    7355
NP_055637                                                              16    7355
NP_001005850                                                           16    7355
XP_005276733                                                           16    7355
XP_005259517                                                           16    7355
XP_005259440                                                           16    7355
XP_005259439                                                           16    7355
XP_006716981                                                           16    7355
XP_006716980                                                           16    7355
XP_005272572                                                           16    7355
XP_005272573                                                           16    7355
XP_005263436                                                           16    7355
XP_005263435                                                           16    7355
XP_006712608                                                           16    7355
NP_149350                                                              16    7355
NP_001128661                                                           15    9605
XP_006712544                                                           15    9605
XP_006712543                                                           15    9605
XP_005246573                                                           15    9605
XP_006712542                                                           15    9605
XP_006712541                                                           15    9605
NP_001128660                                                           15    9605
NP_001128659                                                           15    9605
NP_004495                                                              15    9605
>NP_001245210
          Length = 868

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VASFAHVSN 9
           V SFAHVSN
Sbjct: 539 VVSFAHVSN 547
>NP_000242
          Length = 934

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VASFAHVSN 9
           V SFAHVSN
Sbjct: 605 VVSFAHVSN 613
>XP_005264389
          Length = 921

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VASFAHVSN 9
           V SFAHVSN
Sbjct: 605 VVSFAHVSN 613
>NP_060016
          Length = 510

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ASFAHVSN 9
           ASFAH+ N
Sbjct: 147 ASFAHLLN 154
>NP_001036212
          Length = 529

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  ASFAHVSN 9
          +SFAH+ N
Sbjct: 45 SSFAHIPN 52
>NP_001036194
          Length = 529

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  ASFAHVSN 9
          +SFAH+ N
Sbjct: 45 SSFAHIPN 52
>NP_005239
          Length = 529

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  ASFAHVSN 9
          +SFAH+ N
Sbjct: 45 SSFAHIPN 52
>XP_006710515
          Length = 529

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  ASFAHVSN 9
          +SFAH+ N
Sbjct: 45 SSFAHIPN 52
>NP_000042
          Length = 3056

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    ASFAHVSN 9
            A+FA++SN
Sbjct: 1388 ATFAYISN 1395
>NP_001073982
          Length = 545

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SFAHVSN 9
           +FAH+SN
Sbjct: 308 TFAHLSN 314
>NP_031392
          Length = 1463

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VASFAHV 7
           +ASFAH+
Sbjct: 706 LASFAHI 712
>NP_001007268
          Length = 1324

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VASFAHV 7
           +ASFAH+
Sbjct: 706 LASFAHI 712
>NP_001182570
          Length = 1461

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VASFAHV 7
           +ASFAH+
Sbjct: 706 LASFAHI 712
>XP_006718908
          Length = 1708

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  ASFAHVSN 9
          A+FA++SN
Sbjct: 40 ATFAYISN 47
>XP_005271621
          Length = 2708

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    ASFAHVSN 9
            A+FA++SN
Sbjct: 1040 ATFAYISN 1047
>XP_006718907
          Length = 3050

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    ASFAHVSN 9
            A+FA++SN
Sbjct: 1382 ATFAYISN 1389
>XP_006718906
          Length = 3056

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    ASFAHVSN 9
            A+FA++SN
Sbjct: 1388 ATFAYISN 1395
>XP_005271619
          Length = 3056

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    ASFAHVSN 9
            A+FA++SN
Sbjct: 1388 ATFAYISN 1395
>XP_005271618
          Length = 3056

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    ASFAHVSN 9
            A+FA++SN
Sbjct: 1388 ATFAYISN 1395
>XP_005269338
          Length = 545

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SFAHVSN 9
           +FAH+SN
Sbjct: 308 TFAHLSN 314
>XP_005269337
          Length = 545

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SFAHVSN 9
           +FAH+SN
Sbjct: 308 TFAHLSN 314
>XP_005246452
          Length = 776

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  VASFAHV 7
          +ASFAH+
Sbjct: 19 LASFAHI 25
>XP_006712432
          Length = 786

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  VASFAHV 7
          +ASFAH+
Sbjct: 29 LASFAHI 35
>XP_005246450
          Length = 1461

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VASFAHV 7
           +ASFAH+
Sbjct: 706 LASFAHI 712
>XP_005246449
          Length = 1463

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VASFAHV 7
           +ASFAH+
Sbjct: 706 LASFAHI 712
>NP_996763
          Length = 315

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VASFAHV 7
           VAS+AH+
Sbjct: 216 VASYAHI 222
>NP_001267
          Length = 383

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VASFAHVSN 9
          + SFA++SN
Sbjct: 55 IYSFANISN 63
>NP_004035
          Length = 592

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ASFAHVS 8
           ASF HVS
Sbjct: 263 ASFKHVS 269
>NP_055637
          Length = 1032

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VASFAHVS 8
           V SFAH++
Sbjct: 946 VPSFAHIN 953
>NP_001005850
          Length = 537

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ASFAHVSN 9
           A+F+HVS+
Sbjct: 397 AAFSHVSS 404
>XP_005276733
          Length = 330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  ASFAHVSN 9
          +SFAH+S+
Sbjct: 64 SSFAHLSS 71
>XP_005259517
          Length = 330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  ASFAHVSN 9
          +SFAH+S+
Sbjct: 64 SSFAHLSS 71
>XP_005259440
          Length = 537

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ASFAHVSN 9
           A+F+HVS+
Sbjct: 397 AAFSHVSS 404
>XP_005259439
          Length = 537

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ASFAHVSN 9
           A+F+HVS+
Sbjct: 397 AAFSHVSS 404
>XP_006716981
          Length = 1053

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SFAHVS 8
           SFAH+S
Sbjct: 632 SFAHIS 637
>XP_006716980
          Length = 1059

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SFAHVS 8
           SFAH+S
Sbjct: 638 SFAHIS 643
>XP_005272572
          Length = 1059

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SFAHVS 8
           SFAH+S
Sbjct: 638 SFAHIS 643
>XP_005272573
          Length = 1059

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SFAHVS 8
           SFAH+S
Sbjct: 638 SFAHIS 643
>XP_005263436
          Length = 1033

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VASFAHVS 8
           V SFAH++
Sbjct: 947 VPSFAHIN 954
>XP_005263435
          Length = 1033

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VASFAHVS 8
           V SFAH++
Sbjct: 947 VPSFAHIN 954
>XP_006712608
          Length = 533

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ASFAHVS 8
           ASF HVS
Sbjct: 204 ASFKHVS 210
>NP_149350
          Length = 1059

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SFAHVS 8
           SFAH+S
Sbjct: 638 SFAHIS 643
>NP_001128661
          Length = 522

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   ASFAHVSN 9
           A+FAH  N
Sbjct: 240 ANFAHFDN 247
>XP_006712544
          Length = 385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  ASFAHVSN 9
          A+FAH  N
Sbjct: 87 ANFAHFDN 94
>XP_006712543
          Length = 578

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   ASFAHVSN 9
           A+FAH  N
Sbjct: 280 ANFAHFDN 287
>XP_005246573
          Length = 586

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   ASFAHVSN 9
           A+FAH  N
Sbjct: 304 ANFAHFDN 311
>XP_006712542
          Length = 600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   ASFAHVSN 9
           A+FAH  N
Sbjct: 304 ANFAHFDN 311
>XP_006712541
          Length = 602

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   ASFAHVSN 9
           A+FAH  N
Sbjct: 304 ANFAHFDN 311
>NP_001128660
          Length = 560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   ASFAHVSN 9
           A+FAH  N
Sbjct: 280 ANFAHFDN 287
>NP_001128659
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   ASFAHVSN 9
           A+FAH  N
Sbjct: 304 ANFAHFDN 311
>NP_004495
          Length = 562

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   ASFAHVSN 9
           A+FAH  N
Sbjct: 280 ANFAHFDN 287
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.319    0.116    0.317 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 445,824
Number of extensions: 606
Number of successful extensions: 51
Number of sequences better than 10000.0: 51
Number of HSP's gapped: 51
Number of HSP's successfully gapped: 51
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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