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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              21     229
NP_671714                                                              21     229
XP_006715890                                                           21     229
XP_005250160                                                           21     229
XP_005250159                                                           21     229
NP_061136                                                              19     665
XP_006724827                                                           19     665
XP_005276776                                                           17    3301
XP_005262564                                                           17    3301
XP_006710324                                                           17    3301
NP_001276091                                                           16    5631
XP_006721364                                                           16    5631
NP_001276092                                                           16    5631
NP_115645                                                              16    5631
NP_001116434                                                           16    7355
XP_005260765                                                           16    7355
NP_003963                                                              15    9605
XP_005270323                                                           15    9605
XP_005270322                                                           15    9605

>NP_005742
          Length = 3907

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    AMSTQDQH 9
            AMSTQDQH
Sbjct: 2270 AMSTQDQH 2277



>NP_671714
          Length = 3899

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    AMSTQDQH 9
            AMSTQDQH
Sbjct: 2262 AMSTQDQH 2269



>XP_006715890
          Length = 3860

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    AMSTQDQH 9
            AMSTQDQH
Sbjct: 2223 AMSTQDQH 2230



>XP_005250160
          Length = 3939

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    AMSTQDQH 9
            AMSTQDQH
Sbjct: 2302 AMSTQDQH 2309



>XP_005250159
          Length = 3952

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    AMSTQDQH 9
            AMSTQDQH
Sbjct: 2315 AMSTQDQH 2322



>NP_061136
          Length = 904

 Score = 19.2 bits (38), Expect =   665
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   AMSTQDQH 9
           AMST+DQH
Sbjct: 380 AMSTRDQH 387




 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AMSTQDQH 9
           AMST+DQ+
Sbjct: 568 AMSTRDQY 575




 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AMSTQDQH 9
           +MST+DQ+
Sbjct: 615 SMSTRDQY 622




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AMSTQDQH 9
           +MST+D H
Sbjct: 145 SMSTRDLH 152




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MSTQDQH 9
           MST+DQ+
Sbjct: 428 MSTRDQY 434



>XP_006724827
          Length = 904

 Score = 19.2 bits (38), Expect =   665
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   AMSTQDQH 9
           AMST+DQH
Sbjct: 380 AMSTRDQH 387




 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AMSTQDQH 9
           AMST+DQ+
Sbjct: 568 AMSTRDQY 575




 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AMSTQDQH 9
           +MST+DQ+
Sbjct: 615 SMSTRDQY 622




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AMSTQDQH 9
           +MST+D H
Sbjct: 145 SMSTRDLH 152




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MSTQDQH 9
           MST+DQ+
Sbjct: 428 MSTRDQY 434



>XP_005276776
          Length = 991

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AMSTQDQH 9
           AMST+DQ+
Sbjct: 862 AMSTRDQY 869




 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AMSTQDQH 9
           +MST+DQ+
Sbjct: 909 SMSTRDQY 916



>XP_005262564
          Length = 991

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AMSTQDQH 9
           AMST+DQ+
Sbjct: 862 AMSTRDQY 869




 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AMSTQDQH 9
           +MST+DQ+
Sbjct: 909 SMSTRDQY 916



>XP_006710324
          Length = 943

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AMSTQDQH 9
           AMST+DQ+
Sbjct: 814 AMSTRDQY 821




 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AMSTQDQH 9
           +MST+DQ+
Sbjct: 861 SMSTRDQY 868



>NP_001276091
          Length = 874

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   STQDQH 9
           STQD+H
Sbjct: 329 STQDEH 334



>XP_006721364
          Length = 831

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   STQDQH 9
           STQD+H
Sbjct: 329 STQDEH 334



>NP_001276092
          Length = 809

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   STQDQH 9
           STQD+H
Sbjct: 264 STQDEH 269



>NP_115645
          Length = 874

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   STQDQH 9
           STQD+H
Sbjct: 329 STQDEH 334



>NP_001116434
          Length = 342

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VAMSTQDQ 8
          V +STQDQ
Sbjct: 74 VKVSTQDQ 81



>XP_005260765
          Length = 348

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VAMSTQDQ 8
          V +STQDQ
Sbjct: 74 VKVSTQDQ 81



>NP_003963
          Length = 1849

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MSTQDQH 9
           +STQDQ+
Sbjct: 545 LSTQDQN 551



>XP_005270323
          Length = 1519

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MSTQDQH 9
           +STQDQ+
Sbjct: 215 LSTQDQN 221



>XP_005270322
          Length = 1646

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MSTQDQH 9
           +STQDQ+
Sbjct: 342 LSTQDQN 348



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.109    0.288 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 408,649
Number of extensions: 336
Number of successful extensions: 30
Number of sequences better than 10000.0: 19
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)