Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_056099                                                              17    2528
XP_006710565                                                           17    2528
XP_006710564                                                           17    2528
XP_005270743                                                           17    2528
NP_001240622                                                           16    5631
NP_001018081                                                           15    9605
NP_001265179                                                           15    9605
NP_001265180                                                           15    9605
XP_005261449                                                           15    9605
XP_005261448                                                           15    9605
XP_006718577                                                           15    9605
XP_006718576                                                           15    9605
XP_006718575                                                           15    9605
XP_006718573                                                           15    9605
XP_006718572                                                           15    9605
XP_006718571                                                           15    9605
XP_006718570                                                           15    9605
NP_872362                                                              15    9605
NP_001070709                                                           15    9605
>NP_056099
          Length = 3375

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AMLSFQYL 9
           +MLSF+YL
Sbjct: 949 SMLSFEYL 956
>XP_006710565
          Length = 3140

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AMLSFQYL 9
           +MLSF+YL
Sbjct: 657 SMLSFEYL 664
>XP_006710564
          Length = 3432

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AMLSFQYL 9
           +MLSF+YL
Sbjct: 949 SMLSFEYL 956
>XP_005270743
          Length = 3453

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AMLSFQYL 9
           +MLSF+YL
Sbjct: 970 SMLSFEYL 977
>NP_001240622
          Length = 497

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VAMLSFQYL 9
          +AM++ QYL
Sbjct: 70 IAMVTIQYL 78
>NP_001018081
          Length = 1309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VAMLSFQY 8
           +AML FQ+
Sbjct: 583 IAMLRFQW 590
>NP_001265179
          Length = 264

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VAMLSFQYL 9
           V  LS++YL
Sbjct: 251 VTFLSYEYL 259
>NP_001265180
          Length = 246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VAMLSFQYL 9
           V  LS++YL
Sbjct: 233 VTFLSYEYL 241
>XP_005261449
          Length = 1029

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  AMLSFQYL 9
          A L+F+YL
Sbjct: 12 AQLTFEYL 19
>XP_005261448
          Length = 1032

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  AMLSFQYL 9
          A L+F+YL
Sbjct: 12 AQLTFEYL 19
>XP_006718577
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VAMLSFQYL 9
           V  LS++YL
Sbjct: 213 VTFLSYEYL 221
>XP_006718576
          Length = 246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VAMLSFQYL 9
           V  LS++YL
Sbjct: 233 VTFLSYEYL 241
>XP_006718575
          Length = 246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VAMLSFQYL 9
           V  LS++YL
Sbjct: 233 VTFLSYEYL 241
>XP_006718573
          Length = 246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VAMLSFQYL 9
           V  LS++YL
Sbjct: 233 VTFLSYEYL 241
>XP_006718572
          Length = 246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VAMLSFQYL 9
           V  LS++YL
Sbjct: 233 VTFLSYEYL 241
>XP_006718571
          Length = 246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VAMLSFQYL 9
           V  LS++YL
Sbjct: 233 VTFLSYEYL 241
>XP_006718570
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VAMLSFQYL 9
           V  LS++YL
Sbjct: 275 VTFLSYEYL 283
>NP_872362
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VAMLSFQYL 9
           V  LS++YL
Sbjct: 275 VTFLSYEYL 283
>NP_001070709
          Length = 246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VAMLSFQYL 9
           V  LS++YL
Sbjct: 233 VTFLSYEYL 241
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.344    0.143    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 324,191
Number of extensions: 242
Number of successful extensions: 19
Number of sequences better than 10000.0: 19
Number of HSP's gapped: 19
Number of HSP's successfully gapped: 19
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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