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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     509
NP_001075109                                                           20     509
NP_001180291                                                           17    2528
NP_001180290                                                           17    2528
NP_001180289                                                           17    2528
NP_001180292                                                           17    2528
NP_060669                                                              17    2528
XP_005267875                                                           17    2528
XP_005267874                                                           17    2528
XP_006720253                                                           17    2528
NP_004163                                                              17    4312
NP_001160167                                                           17    4312
NP_001276868                                                           17    4312
NP_002934                                                              17    4312
XP_006714551                                                           17    4312
XP_005248399                                                           17    4312
NP_031401                                                              16    5631
NP_036312                                                              16    5631
NP_001257545                                                           16    5631
NP_002754                                                              16    5631
NP_004893                                                              16    5631
NP_001229529                                                           16    5631
NP_001229528                                                           16    5631
NP_909122                                                              16    5631
XP_006723956                                                           16    5631
XP_006723955                                                           16    5631
XP_006723954                                                           16    5631
XP_006723953                                                           16    5631
XP_005273252                                                           16    5631
XP_005273253                                                           16    5631
XP_005273251                                                           16    5631
NP_787050                                                              16    7355
XP_006719865                                                           16    7355
XP_006719864                                                           16    7355
XP_005266391                                                           16    7355
XP_006719863                                                           16    7355
XP_005266390                                                           16    7355
XP_006719862                                                           16    7355
XP_005266389                                                           16    7355
NP_055979                                                              15    9605
NP_068597                                                              15    9605
NP_001242953                                                           15    9605
NP_067049                                                              15    9605
NP_001242955                                                           15    9605
NP_001242954                                                           15    9605
XP_005274209                                                           15    9605
XP_006717997                                                           15    9605
XP_005270071                                                           15    9605
XP_005245848                                                           15    9605
XP_005245847                                                           15    9605

>NP_008835
          Length = 4128

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ALREFFST 9
            ALREFFST
Sbjct: 1835 ALREFFST 1842



>NP_001075109
          Length = 4097

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ALREFFST 9
            ALREFFST
Sbjct: 1835 ALREFFST 1842



>NP_001180291
          Length = 621

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ALREFFST 9
           ALREF++T
Sbjct: 526 ALREFYNT 533



>NP_001180290
          Length = 621

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ALREFFST 9
           ALREF++T
Sbjct: 526 ALREFYNT 533



>NP_001180289
          Length = 621

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ALREFFST 9
           ALREF++T
Sbjct: 526 ALREFYNT 533



>NP_001180292
          Length = 621

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ALREFFST 9
           ALREF++T
Sbjct: 526 ALREFYNT 533



>NP_060669
          Length = 496

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ALREFFST 9
           ALREF++T
Sbjct: 401 ALREFYNT 408



>XP_005267875
          Length = 366

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ALREFFST 9
           ALREF++T
Sbjct: 271 ALREFYNT 278



>XP_005267874
          Length = 496

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ALREFFST 9
           ALREF++T
Sbjct: 401 ALREFYNT 408



>XP_006720253
          Length = 621

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ALREFFST 9
           ALREF++T
Sbjct: 526 ALREFYNT 533



>NP_004163
          Length = 542

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALREFFST 9
           ALREFF +
Sbjct: 266 ALREFFDS 273



>NP_001160167
          Length = 497

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALREFFST 9
           ALREFF +
Sbjct: 266 ALREFFDS 273



>NP_001276868
          Length = 496

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALREFFST 9
           ALREFF +
Sbjct: 220 ALREFFDS 227



>NP_002934
          Length = 548

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VALREFFS 8
          + LREFFS
Sbjct: 75 INLREFFS 82



>XP_006714551
          Length = 542

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALREFFST 9
           ALREFF +
Sbjct: 266 ALREFFDS 273



>XP_005248399
          Length = 542

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALREFFST 9
           ALREFF +
Sbjct: 266 ALREFFDS 273



>NP_031401
          Length = 414

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LREFFS 8
           LREFFS
Sbjct: 207 LREFFS 212




 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LREFFST 9
           L+E+FST
Sbjct: 120 LKEYFST 126



>NP_036312
          Length = 319

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   ALREFF 7
           ALREFF
Sbjct: 213 ALREFF 218



>NP_001257545
          Length = 737

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VALREFFS 8
           + LREFF+
Sbjct: 687 ITLREFFN 694



>NP_002754
          Length = 737

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VALREFFS 8
           + LREFF+
Sbjct: 687 ITLREFFN 694



>NP_004893
          Length = 524

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   LREFFST 9
           L EFFST
Sbjct: 169 LEEFFST 175



>NP_001229529
          Length = 502

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   LREFFST 9
           L EFFST
Sbjct: 147 LEEFFST 153



>NP_001229528
          Length = 508

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   LREFFST 9
           L EFFST
Sbjct: 147 LEEFFST 153



>NP_909122
          Length = 530

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   LREFFST 9
           L EFFST
Sbjct: 169 LEEFFST 175



>XP_006723956
          Length = 373

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  LREFFST 9
          L EFFST
Sbjct: 12 LEEFFST 18



>XP_006723955
          Length = 373

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  LREFFST 9
          L EFFST
Sbjct: 12 LEEFFST 18



>XP_006723954
          Length = 373

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  LREFFST 9
          L EFFST
Sbjct: 12 LEEFFST 18



>XP_006723953
          Length = 373

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  LREFFST 9
          L EFFST
Sbjct: 12 LEEFFST 18



>XP_005273252
          Length = 738

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VALREFFS 8
           + LREFF+
Sbjct: 688 ITLREFFN 695



>XP_005273253
          Length = 738

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VALREFFS 8
           + LREFF+
Sbjct: 688 ITLREFFN 695



>XP_005273251
          Length = 738

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VALREFFS 8
           + LREFF+
Sbjct: 688 ITLREFFN 695



>NP_787050
          Length = 887

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VALREFFST 9
           V LRE F+T
Sbjct: 549 VTLREVFTT 557



>XP_006719865
          Length = 553

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VALREFFST 9
           V LRE F+T
Sbjct: 215 VTLREVFTT 223



>XP_006719864
          Length = 575

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VALREFFST 9
           V LRE F+T
Sbjct: 237 VTLREVFTT 245



>XP_005266391
          Length = 575

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VALREFFST 9
           V LRE F+T
Sbjct: 237 VTLREVFTT 245



>XP_006719863
          Length = 656

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VALREFFST 9
           V LRE F+T
Sbjct: 549 VTLREVFTT 557



>XP_005266390
          Length = 833

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VALREFFST 9
           V LRE F+T
Sbjct: 495 VTLREVFTT 503



>XP_006719862
          Length = 848

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VALREFFST 9
           V LRE F+T
Sbjct: 549 VTLREVFTT 557



>XP_005266389
          Length = 886

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VALREFFST 9
           V LRE F+T
Sbjct: 548 VTLREVFTT 556



>NP_055979
          Length = 1019

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VALREFF 7
           VAL EFF
Sbjct: 688 VALAEFF 694



>NP_068597
          Length = 254

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  REFFST 9
          REFF+T
Sbjct: 33 REFFTT 38



>NP_001242953
          Length = 714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   LREFFST 9
           + EFFST
Sbjct: 693 MEEFFST 699



>NP_067049
          Length = 698

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   LREFFST 9
           + EFFST
Sbjct: 677 MEEFFST 683



>NP_001242955
          Length = 608

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   LREFFST 9
           + EFFST
Sbjct: 587 MEEFFST 593



>NP_001242954
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   LREFFST 9
           + EFFST
Sbjct: 683 MEEFFST 689



>XP_005274209
          Length = 206

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  REFFST 9
          REFF+T
Sbjct: 33 REFFTT 38



>XP_006717997
          Length = 608

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   LREFFST 9
           + EFFST
Sbjct: 587 MEEFFST 593



>XP_005270071
          Length = 624

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   LREFFST 9
           + EFFST
Sbjct: 603 MEEFFST 609



>XP_005245848
          Length = 816

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VALREFF 7
           VAL EFF
Sbjct: 485 VALAEFF 491



>XP_005245847
          Length = 999

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VALREFF 7
           VAL EFF
Sbjct: 668 VALAEFF 674



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.339    0.141    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 448,034
Number of extensions: 657
Number of successful extensions: 51
Number of sequences better than 10000.0: 50
Number of HSP's gapped: 51
Number of HSP's successfully gapped: 51
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)