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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004060                                                              16    5631
NP_067586                                                              16    5631
XP_006723067                                                           16    5631
XP_005258557                                                           16    5631
XP_005258556                                                           16    5631
NP_001538                                                              15    9605

>NP_004060
          Length = 142

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VAKQLLML 8
           V KQLLML
Sbjct: 131 VLKQLLML 138



>NP_067586
          Length = 104

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VAKQLLML 8
           V KQLLML
Sbjct: 93  VLKQLLML 100



>XP_006723067
          Length = 144

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VAKQLLML 8
           V KQLLML
Sbjct: 133 VLKQLLML 140



>XP_005258557
          Length = 156

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VAKQLLML 8
           V KQLLML
Sbjct: 145 VLKQLLML 152



>XP_005258556
          Length = 158

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VAKQLLML 8
           V KQLLML
Sbjct: 147 VLKQLLML 154



>NP_001538
          Length = 472

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VAKQLLML 8
           VAKQLL L
Sbjct: 368 VAKQLLHL 375



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.137    0.315 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 222,585
Number of extensions: 88
Number of successful extensions: 6
Number of sequences better than 10000.0: 6
Number of HSP's gapped: 6
Number of HSP's successfully gapped: 6
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)