Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     299
NP_001075109                                                           20     299
NP_667345                                                              17    4312
NP_002495                                                              17    4312
NP_667344                                                              17    4312
XP_006716839                                                           17    4312
XP_005251532                                                           17    4312
XP_005251531                                                           17    4312
XP_005251530                                                           17    4312
NP_005236                                                              16    5631
NP_940907                                                              16    5631
XP_006714202                                                           16    5631
XP_005262892                                                           16    5631
XP_005262891                                                           16    5631
NP_001186491                                                           16    7355
NP_003551                                                              16    7355
NP_001004426                                                           16    7355
NP_705833                                                              16    7355
XP_005261828                                                           16    7355
XP_006724395                                                           16    7355
XP_005261823                                                           16    7355
XP_005261822                                                           16    7355
XP_005261821                                                           16    7355
XP_005247174                                                           16    7355
NP_005903                                                              15    9605
NP_002377                                                              15    9605
NP_001119809                                                           15    9605
XP_005245531                                                           15    9605
NP_001131080                                                           15    9605
NP_064512                                                              15    9605

>NP_008835
          Length = 4128

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   VAKNAEMH 8
           VAKNAEMH
Sbjct: 327 VAKNAEMH 334



>NP_001075109
          Length = 4097

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   VAKNAEMH 8
           VAKNAEMH
Sbjct: 327 VAKNAEMH 334



>NP_667345
          Length = 833

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   VAKNAEMHT 9
           V KN E HT
Sbjct: 336 VLKNVETHT 344



>NP_002495
          Length = 1120

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   VAKNAEMHT 9
           V KN E HT
Sbjct: 336 VLKNVETHT 344



>NP_667344
          Length = 1024

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   VAKNAEMHT 9
           V KN E HT
Sbjct: 336 VLKNVETHT 344



>XP_006716839
          Length = 833

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   VAKNAEMHT 9
           V KN E HT
Sbjct: 336 VLKNVETHT 344



>XP_005251532
          Length = 834

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   VAKNAEMHT 9
           V KN E HT
Sbjct: 336 VLKNVETHT 344



>XP_005251531
          Length = 1025

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   VAKNAEMHT 9
           V KN E HT
Sbjct: 336 VLKNVETHT 344



>XP_005251530
          Length = 1121

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   VAKNAEMHT 9
           V KN E HT
Sbjct: 336 VLKNVETHT 344



>NP_005236
          Length = 4588

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    VAKNAEMHT 9
            +A+NA +HT
Sbjct: 2501 LAENAPLHT 2509



>NP_940907
          Length = 1218

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VAKNAEM 7
           VAKN+EM
Sbjct: 729 VAKNSEM 735



>XP_006714202
          Length = 4588

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    VAKNAEMHT 9
            +A+NA +HT
Sbjct: 2501 LAENAPLHT 2509



>XP_005262892
          Length = 4600

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    VAKNAEMHT 9
            +A+NA +HT
Sbjct: 2501 LAENAPLHT 2509



>XP_005262891
          Length = 4600

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    VAKNAEMHT 9
            +A+NA +HT
Sbjct: 2501 LAENAPLHT 2509



>NP_001186491
          Length = 752

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AKNAEM 7
           AKNAEM
Sbjct: 294 AKNAEM 299



>NP_003551
          Length = 806

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AKNAEM 7
           AKNAEM
Sbjct: 294 AKNAEM 299



>NP_001004426
          Length = 752

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AKNAEM 7
           AKNAEM
Sbjct: 294 AKNAEM 299



>NP_705833
          Length = 2970

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VAKNAEMHT 9
           +A+N E HT
Sbjct: 956 MAQNCEAHT 964



>XP_005261828
          Length = 467

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AKNAEM 7
           AKNAEM
Sbjct: 294 AKNAEM 299



>XP_006724395
          Length = 806

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AKNAEM 7
           AKNAEM
Sbjct: 294 AKNAEM 299



>XP_005261823
          Length = 806

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AKNAEM 7
           AKNAEM
Sbjct: 294 AKNAEM 299



>XP_005261822
          Length = 806

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AKNAEM 7
           AKNAEM
Sbjct: 294 AKNAEM 299



>XP_005261821
          Length = 806

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AKNAEM 7
           AKNAEM
Sbjct: 294 AKNAEM 299



>XP_005247174
          Length = 2679

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VAKNAEMHT 9
           +A+N E HT
Sbjct: 665 MAQNCEAHT 673



>NP_005903
          Length = 332

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  VAKNAEMHT 9
          +AKN  +H+
Sbjct: 69 IAKNKNLHS 77



>NP_002377
          Length = 317

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  VAKNAEMHT 9
          +AKN  +H+
Sbjct: 63 IAKNRNLHS 71



>NP_001119809
          Length = 365

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   AKNAEMH 8
           A+N EMH
Sbjct: 310 AENLEMH 316



>XP_005245531
          Length = 409

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   AKNAEMH 8
           A+N EMH
Sbjct: 354 AENLEMH 360



>NP_001131080
          Length = 585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VAKNAEMHT 9
           VAK+ ++HT
Sbjct: 293 VAKHKKIHT 301



>NP_064512
          Length = 390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   AKNAEMH 8
           A+N EMH
Sbjct: 335 AENLEMH 341



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.307    0.109    0.286 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 432,798
Number of extensions: 559
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)