Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001243398                                                           19     869
NP_036462                                                              19     869
NP_001243397                                                           19     869
XP_005269721                                                           19     869
XP_005276813                                                           18    1935
XP_005248455                                                           18    1935
NP_001790                                                              18    1935
NP_000982                                                              17    4312
NP_056507                                                              17    4312
NP_758441                                                              17    4312
NP_055392                                                              16    5631
NP_001258652                                                           16    5631
NP_110420                                                              16    5631
XP_006726853                                                           16    5631
XP_005261530                                                           16    5631
XP_006724279                                                           16    5631
XP_006724278                                                           16    5631
XP_006724277                                                           16    5631
XP_006724276                                                           16    5631
XP_006724275                                                           16    5631
XP_005261529                                                           16    5631
XP_006724274                                                           16    5631
XP_006724273                                                           16    5631
XP_005261528                                                           16    5631
XP_005261527                                                           16    5631
XP_006723596                                                           16    5631
XP_006721182                                                           16    5631
XP_006714860                                                           16    5631
XP_006714859                                                           16    5631
XP_005268570                                                           16    5631
XP_006710111                                                           16    5631
NP_001230913                                                           15    9605
NP_068810                                                              15    9605
NP_001138610                                                           15    9605
NP_001230914                                                           15    9605
NP_079366                                                              15    9605
NP_073715                                                              15    9605
NP_001009                                                              15    9605
NP_005669                                                              15    9605
XP_005257601                                                           15    9605
XP_005257599                                                           15    9605
XP_005257600                                                           15    9605
NP_689497                                                              15    9605
>NP_001243398
          Length = 1781

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VAIKRKRTF 9
           VAIKRKR F
Sbjct: 827 VAIKRKRPF 835
>NP_036462
          Length = 2073

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VAIKRKRTF 9
            VAIKRKR F
Sbjct: 1119 VAIKRKRPF 1127
>NP_001243397
          Length = 1890

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VAIKRKRTF 9
           VAIKRKR F
Sbjct: 936 VAIKRKRPF 944
>XP_005269721
          Length = 2073

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VAIKRKRTF 9
            VAIKRKR F
Sbjct: 1119 VAIKRKRPF 1127
>XP_005276813
          Length = 289

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   VAIKRKRT 8
           +AIKRKRT
Sbjct: 268 LAIKRKRT 275
>XP_005248455
          Length = 289

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   VAIKRKRT 8
           +AIKRKRT
Sbjct: 268 LAIKRKRT 275
>NP_001790
          Length = 346

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   VAIKRKRT 8
           +AIKRKRT
Sbjct: 325 LAIKRKRT 332
>NP_000982
          Length = 137

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VAIKRKRT 8
           V +KRKRT
Sbjct: 124 VMVKRKRT 131
>NP_056507
          Length = 1932

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KRKRTF 9
           KRKRTF
Sbjct: 788 KRKRTF 793
>NP_758441
          Length = 745

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   IKRKRTF 9
           IK+KRTF
Sbjct: 210 IKQKRTF 216
>NP_055392
          Length = 1058

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VAIKRKRTF 9
           VAI+RK+ F
Sbjct: 464 VAIQRKKQF 472
>NP_001258652
          Length = 943

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AIKRKRT 8
           A+KRKRT
Sbjct: 649 AMKRKRT 655
>NP_110420
          Length = 1113

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AIKRKRT 8
           A+KRKRT
Sbjct: 819 AMKRKRT 825
>XP_006726853
          Length = 707

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AIKRKRTF 9
           ++KR+RTF
Sbjct: 221 SLKRQRTF 228
>XP_005261530
          Length = 784

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VAIKRKRTF 9
          VAI+RK+ F
Sbjct: 59 VAIQRKKQF 67
>XP_006724279
          Length = 802

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VAIKRKRTF 9
          VAI+RK+ F
Sbjct: 77 VAIQRKKQF 85
>XP_006724278
          Length = 978

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VAIKRKRTF 9
           VAI+RK+ F
Sbjct: 501 VAIQRKKQF 509
>XP_006724277
          Length = 995

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VAIKRKRTF 9
           VAI+RK+ F
Sbjct: 501 VAIQRKKQF 509
>XP_006724276
          Length = 1058

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VAIKRKRTF 9
           VAI+RK+ F
Sbjct: 464 VAIQRKKQF 472
>XP_006724275
          Length = 1060

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VAIKRKRTF 9
           VAI+RK+ F
Sbjct: 501 VAIQRKKQF 509
>XP_005261529
          Length = 1095

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VAIKRKRTF 9
           VAI+RK+ F
Sbjct: 501 VAIQRKKQF 509
>XP_006724274
          Length = 1189

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VAIKRKRTF 9
           VAI+RK+ F
Sbjct: 464 VAIQRKKQF 472
>XP_006724273
          Length = 1189

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VAIKRKRTF 9
           VAI+RK+ F
Sbjct: 464 VAIQRKKQF 472
>XP_005261528
          Length = 1189

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VAIKRKRTF 9
           VAI+RK+ F
Sbjct: 464 VAIQRKKQF 472
>XP_005261527
          Length = 1226

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VAIKRKRTF 9
           VAI+RK+ F
Sbjct: 501 VAIQRKKQF 509
>XP_006723596
          Length = 707

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AIKRKRTF 9
           ++KR+RTF
Sbjct: 221 SLKRQRTF 228
>XP_006721182
          Length = 190

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  VAIKRKRT 8
          VAI+RKR+
Sbjct: 19 VAIRRKRS 26
>XP_006714860
          Length = 1146

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AIKRKRT 8
           A+KRKRT
Sbjct: 852 AMKRKRT 858
>XP_006714859
          Length = 1188

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AIKRKRT 8
           A+KRKRT
Sbjct: 894 AMKRKRT 900
>XP_005268570
          Length = 1188

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AIKRKRT 8
           A+KRKRT
Sbjct: 894 AMKRKRT 900
>XP_006710111
          Length = 566

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  AIKRKRTF 9
          ++KR+RTF
Sbjct: 80 SLKRQRTF 87
>NP_001230913
          Length = 482

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KRKRTF 9
           KRKRT+
Sbjct: 301 KRKRTY 306
>NP_068810
          Length = 551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KRKRTF 9
           KRKRT+
Sbjct: 301 KRKRTY 306
>NP_001138610
          Length = 548

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KRKRTF 9
           KRKRT+
Sbjct: 298 KRKRTY 303
>NP_001230914
          Length = 448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KRKRTF 9
           KRKRT+
Sbjct: 301 KRKRTY 306
>NP_079366
          Length = 1123

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KRKRTF 9
           KRK+TF
Sbjct: 811 KRKKTF 816
>NP_073715
          Length = 71

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  VAIKRKRT 8
          V +KR+RT
Sbjct: 23 VQVKRRRT 30
>NP_001009
          Length = 145

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  KRKRTF 9
          K+KRTF
Sbjct: 7  KKKRTF 12
>NP_005669
          Length = 71

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  VAIKRKRT 8
          V +KR+RT
Sbjct: 23 VQVKRRRT 30
>XP_005257601
          Length = 1123

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KRKRTF 9
           KRK+TF
Sbjct: 811 KRKKTF 816
>XP_005257599
          Length = 1123

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KRKRTF 9
           KRK+TF
Sbjct: 811 KRKKTF 816
>XP_005257600
          Length = 1123

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KRKRTF 9
           KRK+TF
Sbjct: 811 KRKKTF 816
>NP_689497
          Length = 233

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  AIKRKRTF 9
          A+KRK+ F
Sbjct: 68 ALKRKKRF 75
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.341    0.143    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 458,011
Number of extensions: 411
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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