Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_775771                                                              20     509
NP_115927                                                              20     509
XP_006720466                                                           20     509
XP_005254230                                                           20     509
XP_006720465                                                           20     509
XP_005254228                                                           20     509
XP_006715292                                                           20     509
NP_694536                                                              17    3301
NP_003788                                                              17    3301
XP_005274430                                                           17    3301
XP_006718794                                                           17    3301
XP_006718793                                                           17    3301
XP_005250261                                                           17    3301
XP_005250260                                                           17    3301
NP_690852                                                              17    3301
NP_444282                                                              16    5631
NP_004268                                                              16    5631
NP_001190981                                                           16    5631
NP_001190980                                                           16    5631
XP_005273812                                                           16    5631
XP_006715337                                                           16    5631
XP_005249541                                                           16    5631
XP_005249540                                                           16    5631
NP_001108091                                                           16    7355
NP_001108092                                                           16    7355
NP_001108090                                                           16    7355
NP_002397                                                              16    7355
NP_001108089                                                           16    7355
NP_001264162                                                           16    7355
NP_003264                                                              16    7355
XP_006714930                                                           16    7355
XP_006714929                                                           16    7355
XP_005265973                                                           16    7355
XP_005265972                                                           16    7355
NP_001243342                                                           15    9605
NP_001035196                                                           15    9605
NP_061028                                                              15    9605
NP_115745                                                              15    9605
NP_055816                                                              15    9605
NP_004808                                                              15    9605
NP_001164101                                                           15    9605
NP_963923                                                              15    9605
NP_001073276                                                           15    9605
NP_001073275                                                           15    9605
NP_055039                                                              15    9605
NP_056493                                                              15    9605
NP_001163886                                                           15    9605
NP_036263                                                              15    9605
XP_005276461                                                           15    9605
XP_006719869                                                           15    9605
XP_006719868                                                           15    9605
XP_005266408                                                           15    9605
XP_005266407                                                           15    9605
XP_005266403                                                           15    9605
XP_006719867                                                           15    9605
XP_006719866                                                           15    9605
XP_005266405                                                           15    9605
XP_005266404                                                           15    9605
XP_006719703                                                           15    9605
XP_005254005                                                           15    9605
XP_005252372                                                           15    9605
XP_006717387                                                           15    9605
XP_005252371                                                           15    9605
XP_005252370                                                           15    9605
XP_005252369                                                           15    9605
XP_005252368                                                           15    9605
XP_006714394                                                           15    9605
XP_005263325                                                           15    9605
XP_005263324                                                           15    9605
NP_001163885                                                           15    9605
NP_001163887                                                           15    9605
>NP_775771
          Length = 1244

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VAGFRGMVR 9
           VAGFRG VR
Sbjct: 183 VAGFRGTVR 191
>NP_115927
          Length = 1321

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VAGFRGMVR 9
           VAGFRG VR
Sbjct: 196 VAGFRGTVR 204
>XP_006720466
          Length = 1175

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VAGFRGMVR 9
           VAGFRG VR
Sbjct: 114 VAGFRGTVR 122
>XP_005254230
          Length = 1175

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VAGFRGMVR 9
           VAGFRG VR
Sbjct: 114 VAGFRGTVR 122
>XP_006720465
          Length = 1239

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VAGFRGMVR 9
           VAGFRG VR
Sbjct: 178 VAGFRGTVR 186
>XP_005254228
          Length = 1250

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VAGFRGMVR 9
           VAGFRG VR
Sbjct: 189 VAGFRGTVR 197
>XP_006715292
          Length = 759

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VAGFRGMVR 9
           VAGFRG VR
Sbjct: 183 VAGFRGTVR 191
>NP_694536
          Length = 400

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VAGFRGMVR 9
           VAG RG++R
Sbjct: 165 VAGSRGIIR 173
>NP_003788
          Length = 441

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VAGFRGMVR 9
           VAG RG++R
Sbjct: 165 VAGSRGIIR 173
>XP_005274430
          Length = 361

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VAGFRGMVR 9
           VAG RG++R
Sbjct: 165 VAGSRGIIR 173
>XP_006718794
          Length = 390

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VAGFRGMVR 9
           VAG RG++R
Sbjct: 165 VAGSRGIIR 173
>XP_006718793
          Length = 403

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VAGFRGMVR 9
           VAG RG++R
Sbjct: 165 VAGSRGIIR 173
>XP_005250261
          Length = 495

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AGFRGMVR 9
           AG RG+VR
Sbjct: 216 AGLRGLVR 223
>XP_005250260
          Length = 595

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AGFRGMVR 9
           AG RG+VR
Sbjct: 368 AGLRGLVR 375
>NP_690852
          Length = 647

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AGFRGMVR 9
           AG RG+VR
Sbjct: 368 AGLRGLVR 375
>NP_444282
          Length = 780

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VAGFRGMVR 9
           + GFR M+R
Sbjct: 433 IQGFREMIR 441
>NP_004268
          Length = 323

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AGFRGMVR 9
          A +RGMVR
Sbjct: 66 APYRGMVR 73
>NP_001190981
          Length = 245

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AGFRGMVR 9
          A +RGMVR
Sbjct: 66 APYRGMVR 73
>NP_001190980
          Length = 300

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AGFRGMVR 9
          A +RGMVR
Sbjct: 66 APYRGMVR 73
>XP_005273812
          Length = 649

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VAGFRGMVR 9
           + GFR M+R
Sbjct: 302 IQGFREMIR 310
>XP_006715337
          Length = 266

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AGFRGMVR 9
          A +RGMVR
Sbjct: 66 APYRGMVR 73
>XP_005249541
          Length = 275

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AGFRGMVR 9
          A +RGMVR
Sbjct: 18 APYRGMVR 25
>XP_005249540
          Length = 310

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AGFRGMVR 9
          A +RGMVR
Sbjct: 66 APYRGMVR 73
>NP_001108091
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AGFRGMV 8
           AG+RG+V
Sbjct: 414 AGYRGIV 420
>NP_001108092
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AGFRGMV 8
           AG+RG+V
Sbjct: 414 AGYRGIV 420
>NP_001108090
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AGFRGMV 8
           AG+RG+V
Sbjct: 414 AGYRGIV 420
>NP_002397
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AGFRGMV 8
           AG+RG+V
Sbjct: 414 AGYRGIV 420
>NP_001108089
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AGFRGMV 8
           AG+RG+V
Sbjct: 414 AGYRGIV 420
>NP_001264162
          Length = 391

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VAGFRGMVR 9
           V+G+ GMVR
Sbjct: 312 VSGWWGMVR 320
>NP_003264
          Length = 418

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VAGFRGMVR 9
           V+G+ GMVR
Sbjct: 339 VSGWWGMVR 347
>XP_006714930
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AGFRGMV 8
           AG+RG+V
Sbjct: 414 AGYRGIV 420
>XP_006714929
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AGFRGMV 8
           AG+RG+V
Sbjct: 414 AGYRGIV 420
>XP_005265973
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AGFRGMV 8
           AG+RG+V
Sbjct: 414 AGYRGIV 420
>XP_005265972
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AGFRGMV 8
           AG+RG+V
Sbjct: 414 AGYRGIV 420
>NP_001243342
          Length = 253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  FRGMVR 9
          FRGM+R
Sbjct: 68 FRGMLR 73
>NP_001035196
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  FRGMVR 9
          FRGM+R
Sbjct: 88 FRGMLR 93
>NP_061028
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3 GFRGMVR 9
         G RGM+R
Sbjct: 2 GIRGMLR 8
>NP_115745
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  FRGMVR 9
          FRGM+R
Sbjct: 88 FRGMLR 93
>NP_055816
          Length = 3664

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VAGFRG 6
           V+GFRG
Sbjct: 104 VSGFRG 109
>NP_004808
          Length = 1190

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FRGMVR 9
           FRG+VR
Sbjct: 563 FRGLVR 568
>NP_001164101
          Length = 993

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FRGMVR 9
           FRG+VR
Sbjct: 563 FRGLVR 568
>NP_963923
          Length = 1043

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FRGMVR 9
           FRG+VR
Sbjct: 563 FRGLVR 568
>NP_001073276
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   VAGFRGM 7
           ++GF+GM
Sbjct: 345 ISGFQGM 351
>NP_001073275
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   VAGFRGM 7
           ++GF+GM
Sbjct: 345 ISGFQGM 351
>NP_055039
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   VAGFRGM 7
           ++GF+GM
Sbjct: 345 ISGFQGM 351
>NP_056493
          Length = 2946

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3    GFRGM 7
            GFRGM
Sbjct: 1306 GFRGM 1310
>NP_001163886
          Length = 1157

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FRGMVR 9
           FRG+VR
Sbjct: 567 FRGLVR 572
>NP_036263
          Length = 224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  VAGFRGMV 8
          V GF GMV
Sbjct: 12 VLGFLGMV 19
>XP_005276461
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   VAGFRGM 7
           ++GF+GM
Sbjct: 345 ISGFQGM 351
>XP_006719869
          Length = 2943

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3    GFRGM 7
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_006719868
          Length = 2938

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3    GFRGM 7
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_005266408
          Length = 1934

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3    GFRGM 7
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_005266407
          Length = 2641

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3   GFRGM 7
           GFRGM
Sbjct: 980 GFRGM 984
>XP_005266403
          Length = 2941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3    GFRGM 7
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_006719867
          Length = 2961

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3    GFRGM 7
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_006719866
          Length = 2962

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3    GFRGM 7
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_005266405
          Length = 2964

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3    GFRGM 7
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_005266404
          Length = 2967

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3    GFRGM 7
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_006719703
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  FRGMVR 9
          FRGM+R
Sbjct: 88 FRGMLR 93
>XP_005254005
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  FRGMVR 9
          FRGM+R
Sbjct: 88 FRGMLR 93
>XP_005252372
          Length = 1102

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FRGMVR 9
           FRG+VR
Sbjct: 622 FRGLVR 627
>XP_006717387
          Length = 1188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FRGMVR 9
           FRG+VR
Sbjct: 561 FRGLVR 566
>XP_005252371
          Length = 1194

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FRGMVR 9
           FRG+VR
Sbjct: 567 FRGLVR 572
>XP_005252370
          Length = 1212

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FRGMVR 9
           FRG+VR
Sbjct: 622 FRGLVR 627
>XP_005252369
          Length = 1213

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FRGMVR 9
           FRG+VR
Sbjct: 586 FRGLVR 591
>XP_005252368
          Length = 1249

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FRGMVR 9
           FRG+VR
Sbjct: 622 FRGLVR 627
>XP_006714394
          Length = 960

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  VAGFRG 6
          V+GFRG
Sbjct: 26 VSGFRG 31
>XP_005263325
          Length = 970

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  VAGFRG 6
          V+GFRG
Sbjct: 26 VSGFRG 31
>XP_005263324
          Length = 1004

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  VAGFRG 6
          V+GFRG
Sbjct: 26 VSGFRG 31
>NP_001163885
          Length = 1020

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FRGMVR 9
           FRG+VR
Sbjct: 540 FRGLVR 545
>NP_001163887
          Length = 1221

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FRGMVR 9
           FRG+VR
Sbjct: 594 FRGLVR 599
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.343    0.154    0.467 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 499,441
Number of extensions: 885
Number of successful extensions: 71
Number of sequences better than 10000.0: 71
Number of HSP's gapped: 71
Number of HSP's successfully gapped: 71
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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