Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     869
NP_001075109                                                           19     869
NP_258411                                                              17    3301
NP_001363                                                              17    3301
XP_005257845                                                           17    3301
XP_005257844                                                           17    3301
XP_006721530                                                           17    3301
XP_006721529                                                           17    3301
XP_006721528                                                           17    3301
NP_004557                                                              16    5631
NP_001138915                                                           16    5631
NP_001269559                                                           16    5631
NP_001193665                                                           16    5631
NP_061327                                                              16    5631
NP_057227                                                              16    5631
XP_005252521                                                           16    5631
XP_005252520                                                           16    5631
XP_005249598                                                           16    5631
XP_005249596                                                           16    5631
XP_005249594                                                           16    5631
NP_057336                                                              16    7355
NP_660183                                                              16    7355
NP_037390                                                              16    7355
NP_005500                                                              16    7355
NP_001277250                                                           16    7355
XP_006714623                                                           16    7355
XP_005271970                                                           16    7355
XP_005271969                                                           16    7355
XP_005271967                                                           16    7355
XP_005271966                                                           16    7355
XP_006712120                                                           16    7355
XP_005264512                                                           16    7355
XP_005264511                                                           16    7355
XP_006712119                                                           16    7355
XP_005264510                                                           16    7355
XP_006712118                                                           16    7355
XP_005264509                                                           16    7355
XP_005264508                                                           16    7355
XP_006712117                                                           16    7355
XP_005264507                                                           16    7355
XP_005264506                                                           16    7355
NP_005853                                                              15    9605
NP_001035706                                                           15    9605
NP_078863                                                              15    9605
NP_004165                                                              15    9605
NP_001271280                                                           15    9605
XP_005255000                                                           15    9605
XP_005254998                                                           15    9605
XP_006713534                                                           15    9605
XP_005271253                                                           15    9605
NP_787107                                                              15    9605
NP_001013665                                                           15    9605
>NP_008835
          Length = 4128

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    VAFFLESI 8
            VAFFLESI
Sbjct: 1294 VAFFLESI 1301
>NP_001075109
          Length = 4097

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    VAFFLESI 8
            VAFFLESI
Sbjct: 1294 VAFFLESI 1301
>NP_258411
          Length = 638

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AFFLESI 8
           AFFLES+
Sbjct: 612 AFFLESV 618
>NP_001363
          Length = 4486

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VAFFLESI 8
            VAFFLE +
Sbjct: 1488 VAFFLEEV 1495
>XP_005257845
          Length = 400

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AFFLESI 8
           AFFLES+
Sbjct: 374 AFFLESV 380
>XP_005257844
          Length = 400

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AFFLESI 8
           AFFLES+
Sbjct: 374 AFFLESV 380
>XP_006721530
          Length = 2746

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VAFFLESI 8
            VAFFLE +
Sbjct: 1488 VAFFLEEV 1495
>XP_006721529
          Length = 2817

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VAFFLESI 8
            VAFFLE +
Sbjct: 1488 VAFFLEEV 1495
>XP_006721528
          Length = 3869

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VAFFLESI 8
            VAFFLE +
Sbjct: 1488 VAFFLEEV 1495
>NP_004557
          Length = 520

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AFFLESI 8
           AFF+ES+
Sbjct: 148 AFFIESV 154
>NP_001138915
          Length = 500

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AFFLESI 8
           AFF+ES+
Sbjct: 128 AFFIESV 134
>NP_001269559
          Length = 534

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AFFLESI 8
           AFF+ES+
Sbjct: 162 AFFIESV 168
>NP_001193665
          Length = 372

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  FFLESIG 9
          FFLES G
Sbjct: 25 FFLESAG 31
>NP_061327
          Length = 339

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  FFLESIG 9
          FFLES G
Sbjct: 25 FFLESAG 31
>NP_057227
          Length = 370

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  FFLESIG 9
          FFLES G
Sbjct: 25 FFLESAG 31
>XP_005252521
          Length = 462

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AFFLESI 8
           AFF+ES+
Sbjct: 148 AFFIESV 154
>XP_005252520
          Length = 514

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AFFLESI 8
           AFF+ES+
Sbjct: 148 AFFIESV 154
>XP_005249598
          Length = 1027

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VAFFLESI 8
          V FFLES+
Sbjct: 8  VRFFLESL 15
>XP_005249596
          Length = 1035

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VAFFLESI 8
          V FFLES+
Sbjct: 8  VRFFLESL 15
>XP_005249594
          Length = 1040

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VAFFLESI 8
          V FFLES+
Sbjct: 8  VRFFLESL 15
>NP_057336
          Length = 4857

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VAFFLESI 8
            VAFFL+ I
Sbjct: 3764 VAFFLQCI 3771
>NP_660183
          Length = 176

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VAFFLESIG 9
          V+F+LE+ G
Sbjct: 57 VSFYLETYG 65
>NP_037390
          Length = 195

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VAFFLESIG 9
          V+F+LE+ G
Sbjct: 57 VSFYLETYG 65
>NP_005500
          Length = 3027

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FFLESI 8
           FFLESI
Sbjct: 107 FFLESI 112
>NP_001277250
          Length = 3048

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FFLESI 8
           FFLESI
Sbjct: 107 FFLESI 112
>XP_006714623
          Length = 1625

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FFLESI 8
           FFLESI
Sbjct: 107 FFLESI 112
>XP_005271970
          Length = 2931

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FFLESI 8
           FFLESI
Sbjct: 107 FFLESI 112
>XP_005271969
          Length = 3020

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  FFLESI 8
          FFLESI
Sbjct: 41 FFLESI 46
>XP_005271967
          Length = 3065

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FFLESI 8
           FFLESI
Sbjct: 107 FFLESI 112
>XP_005271966
          Length = 3086

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FFLESI 8
           FFLESI
Sbjct: 107 FFLESI 112
>XP_006712120
          Length = 4812

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VAFFLESI 8
            VAFFL+ I
Sbjct: 3719 VAFFLQCI 3726
>XP_005264512
          Length = 4840

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VAFFLESI 8
            VAFFL+ I
Sbjct: 3747 VAFFLQCI 3754
>XP_005264511
          Length = 4847

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VAFFLESI 8
            VAFFL+ I
Sbjct: 3791 VAFFLQCI 3798
>XP_006712119
          Length = 4851

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VAFFLESI 8
            VAFFL+ I
Sbjct: 3758 VAFFLQCI 3765
>XP_005264510
          Length = 4861

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VAFFLESI 8
            VAFFL+ I
Sbjct: 3768 VAFFLQCI 3775
>XP_006712118
          Length = 4864

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VAFFLESI 8
            VAFFL+ I
Sbjct: 3771 VAFFLQCI 3778
>XP_005264509
          Length = 4870

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VAFFLESI 8
            VAFFL+ I
Sbjct: 3777 VAFFLQCI 3784
>XP_005264508
          Length = 4875

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VAFFLESI 8
            VAFFL+ I
Sbjct: 3782 VAFFLQCI 3789
>XP_006712117
          Length = 4878

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VAFFLESI 8
            VAFFL+ I
Sbjct: 3785 VAFFLQCI 3792
>XP_005264507
          Length = 4880

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VAFFLESI 8
            VAFFL+ I
Sbjct: 3787 VAFFLQCI 3794
>XP_005264506
          Length = 4884

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    VAFFLESI 8
            VAFFL+ I
Sbjct: 3791 VAFFLQCI 3798
>NP_005853
          Length = 1258

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VAFFLES 7
           V FFLES
Sbjct: 469 VLFFLES 475
>NP_001035706
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FFLESIG 9
           FFL++IG
Sbjct: 497 FFLKNIG 503
>NP_078863
          Length = 277

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  VAFFLESIG 9
          + FF ES+G
Sbjct: 74 MVFFSESLG 82
>NP_004165
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VAFFLESIG 9
           V+FF+ S+G
Sbjct: 252 VSFFVVSLG 260
>NP_001271280
          Length = 825

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VAFFLESIG 9
           V+FF+ S+G
Sbjct: 252 VSFFVVSLG 260
>XP_005255000
          Length = 721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FFLESIG 9
           FFL++IG
Sbjct: 461 FFLKNIG 467
>XP_005254998
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FFLESIG 9
           FFL++IG
Sbjct: 497 FFLKNIG 503
>XP_006713534
          Length = 1222

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VAFFLES 7
           V FFLES
Sbjct: 433 VLFFLES 439
>XP_005271253
          Length = 204

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1 VAFFLESIG 9
         + FF ES+G
Sbjct: 1 MVFFSESLG 9
>NP_787107
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  FFLESIG 9
          FFL S+G
Sbjct: 34 FFLASVG 40
>NP_001013665
          Length = 98

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  FFLESIG 9
          FF+ES G
Sbjct: 76 FFIESCG 82
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.337    0.154    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 354,130
Number of extensions: 343
Number of successful extensions: 52
Number of sequences better than 10000.0: 52
Number of HSP's gapped: 52
Number of HSP's successfully gapped: 52
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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