Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           19     869
NP_000242                                                              19     869
XP_005264389                                                           19     869
NP_002681                                                              16    5631
XP_005273594                                                           16    5631
XP_005273593                                                           16    5631
XP_005273592                                                           16    5631
NP_003240                                                              16    7355
NP_055087                                                              15    9605
NP_001247421                                                           15    9605
NP_001247423                                                           15    9605
NP_001247422                                                           15    9605
NP_002897                                                              15    9605
NP_001247424                                                           15    9605
NP_002285                                                              15    9605
NP_054701                                                              15    9605
NP_001116078                                                           15    9605
NP_006763                                                              15    9605
XP_005254195                                                           15    9605
XP_005254194                                                           15    9605
>NP_001245210
          Length = 868

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VAELANFTK 9
           VAELANF K
Sbjct: 764 VAELANFPK 772
>NP_000242
          Length = 934

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VAELANFTK 9
           VAELANF K
Sbjct: 830 VAELANFPK 838
>XP_005264389
          Length = 921

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VAELANFTK 9
           VAELANF K
Sbjct: 830 VAELANFPK 838
>NP_002681
          Length = 335

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VAELANFTK 9
          + ELANF K
Sbjct: 19 LTELANFEK 27
>XP_005273594
          Length = 306

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VAELANFTK 9
          + ELANF K
Sbjct: 19 LTELANFEK 27
>XP_005273593
          Length = 341

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VAELANFTK 9
          + ELANF K
Sbjct: 19 LTELANFEK 27
>XP_005273592
          Length = 370

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VAELANFTK 9
          + ELANF K
Sbjct: 19 LTELANFEK 27
>NP_003240
          Length = 689

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AELANFTK 9
           A +ANFTK
Sbjct: 447 AMVANFTK 454
>NP_055087
          Length = 1686

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VAELANF 7
           VAE ANF
Sbjct: 730 VAEAANF 736
>NP_001247421
          Length = 604

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AELANFT 8
           AELA FT
Sbjct: 419 AELAEFT 425
>NP_001247423
          Length = 447

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AELANFT 8
           AELA FT
Sbjct: 283 AELAEFT 289
>NP_001247422
          Length = 604

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AELANFT 8
           AELA FT
Sbjct: 419 AELAEFT 425
>NP_002897
          Length = 583

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AELANFT 8
           AELA FT
Sbjct: 419 AELAEFT 425
>NP_001247424
          Length = 257

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  AELANFT 8
          AELA FT
Sbjct: 72 AELAEFT 78
>NP_002285
          Length = 410

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LANFTK 9
           +ANFTK
Sbjct: 99  IANFTK 104
>NP_054701
          Length = 410

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LANFTK 9
           +ANFTK
Sbjct: 99  IANFTK 104
>NP_001116078
          Length = 411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LANFTK 9
           +ANFTK
Sbjct: 99  IANFTK 104
>NP_006763
          Length = 1343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VAELANFTK 9
           +  LANF+K
Sbjct: 630 IQNLANFSK 638
>XP_005254195
          Length = 1155

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VAELANF 7
           VAE ANF
Sbjct: 196 VAEAANF 202
>XP_005254194
          Length = 1689

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VAELANF 7
           VAE ANF
Sbjct: 730 VAEAANF 736
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.124    0.313 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 377,492
Number of extensions: 268
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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